PMID- 37435031
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230718
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 11
DP  - 2023
TI  - Regulation of mitochondrial morphology and cristae architecture by the TLR4 
      pathway in human skeletal muscle.
PG  - 1212779
LID - 10.3389/fcell.2023.1212779 [doi]
LID - 1212779
AB  - In skeletal muscle (SkM), a reduced mitochondrial elongate phenotype is 
      associated with several metabolic disorders like type 2 diabetes mellitus (T2DM). 
      However, the mechanisms contributing to this reduction in mitochondrial elongate 
      phenotype in SkM have not been fully elucidated. It has recently been shown in a 
      SkM cell line that toll-like receptor 4 (TLR4) contributes to the regulation of 
      mitochondrial morphology. However, this has not been investigated in human SkM. 
      Here we found that in human SkM biopsies, TLR4 protein correlated negatively with 
      Opa1 (pro-mitochondrial fusion protein). Moreover, the incubation of human 
      myotubes with LPS reduced mitochondrial size and elongation and induced abnormal 
      mitochondrial cristae, which was prevented with the co-incubation of LPS with 
      TAK(242). Finally, T2DM myotubes were found to have reduced mitochondrial 
      elongation and mitochondrial cristae density. Mitochondrial morphology, membrane 
      structure, and insulin-stimulated glucose uptake were restored to healthy levels 
      in T2DM myotubes treated with TAK(242). In conclusion, mitochondrial morphology 
      and mitochondrial cristae seem to be regulated by the TLR4 pathway in human SkM. 
      Those mitochondrial alterations might potentially contribute to insulin 
      resistance in the SkM of patients with T2DM.
CI  - Copyright (c) 2023 Castro-Sepulveda, Tunon-Suarez, Rosales-Soto, Vargas-Foitzick, 
      Deldicque and Zbinden-Foncea.
FAU - Castro-Sepulveda, Mauricio
AU  - Castro-Sepulveda M
AD  - Laboratorio de Fisiologia del Ejercicio y Metabolismo, Escuela de Kinesiologia, 
      Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
FAU - Tunon-Suarez, Mauro
AU  - Tunon-Suarez M
AD  - Laboratorio de Fisiologia del Ejercicio y Metabolismo, Escuela de Kinesiologia, 
      Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
FAU - Rosales-Soto, Giovanni
AU  - Rosales-Soto G
AD  - Facultad de Ciencias de la Educacion, Universidad San Sebastian, Sede Bellavista, 
      Santiago, Chile.
FAU - Vargas-Foitzick, Ronald
AU  - Vargas-Foitzick R
AD  - Laboratorio de Fisiologia del Ejercicio y Metabolismo, Escuela de Kinesiologia, 
      Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
FAU - Deldicque, Louise
AU  - Deldicque L
AD  - Institute of Neuroscience, UCLouvain, Ottignies-Louvain-la- Neuve, Belgium.
FAU - Zbinden-Foncea, Hermann
AU  - Zbinden-Foncea H
AD  - Laboratorio de Fisiologia del Ejercicio y Metabolismo, Escuela de Kinesiologia, 
      Facultad de Medicina, Universidad Finis Terrae, Santiago, Chile.
AD  - Institute of Neuroscience, UCLouvain, Ottignies-Louvain-la- Neuve, Belgium.
AD  - Facultad de Ciencias de la Salud, Universidad Francisco de Vitoria, Madrid, 
      Espana.
LA  - eng
PT  - Journal Article
DEP - 20230626
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC10332154
OTO - NOTNLM
OT  - Lipopolysaccharide
OT  - TAK242
OT  - mitochondrial dynamics
OT  - mitochondrial nanotunnels
OT  - skeletal muscle function
OT  - type 2 diabetes
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/07/12 06:42
MHDA- 2023/07/12 06:43
PMCR- 2023/01/01
CRDT- 2023/07/12 03:51
PHST- 2023/04/26 00:00 [received]
PHST- 2023/06/12 00:00 [accepted]
PHST- 2023/07/12 06:43 [medline]
PHST- 2023/07/12 06:42 [pubmed]
PHST- 2023/07/12 03:51 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1212779 [pii]
AID - 10.3389/fcell.2023.1212779 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2023 Jun 26;11:1212779. doi: 10.3389/fcell.2023.1212779. 
      eCollection 2023.