PMID- 37436133
OWN - NLM
STAT- MEDLINE
DCOM- 20230919
LR  - 20230919
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 38
IP  - 10
DP  - 2023 Oct
TI  - Sinomenine improves renal fibrosis by regulating mesenchymal stem cell-derived 
      exosomes and affecting autophagy levels.
PG  - 2524-2537
LID - 10.1002/tox.23890 [doi]
AB  - BACKGROUND: This study attempts to investigate the therapeutic effect of 
      sinomenine on renal fibrosis and its mechanism. METHODS: The 8-week-old C57BL/6 
      male mice were randomly divided into sham group, UUO model group, UUO sinomenine 
      group (UUO + Sino 50), UUO + sinomenine group (UUO + Sino 100), UUO + exosome 
      group (exo), and UUO + exo-inhibitor. The pathological changes of kidney were 
      observed by H&E staining, the degree of renal interstitial fibrosis was detected 
      by MASSON and Sirius red staining, and the expressions of fibrosis and autophagy 
      markers were detected by real-time fluorescence quantitative PCR and WB. NTA and 
      electron microscopy were used to analyze exo secretion after sinomenine 
      treatment. RESULTS: Sinomenine could improve the progression of renal fibrosis 
      without causing tissue damage including heart, lungs and liver. Sinomenine could 
      promote autophagosome formation. It could promote the secretion of exosomes from 
      bone marrow mesenchymal stem cells (BMSCs). Sinomine regulates the PI3K-AKT 
      pathway through BMSC-exo carrying miR-204-5p, affecting autophagy level and 
      alleviating the process of renal fibrosis. CONCLUSION: Our study suggests that 
      sinomine could improve the progression of renal fibrosis by influencing the 
      expression of miR-204-5p in BMSC-exo and regulating the PI3K-AKT pathway.
CI  - (c) 2023 Wiley Periodicals LLC.
FAU - Gu, Hongping
AU  - Gu H
AD  - Department of Internal Medicine, Yuyao Traditional Chinese Medicine Hospital, 
      Yuyao, Zhejiang, China.
FAU - Li, Jinrong
AU  - Li J
AD  - Department of Encephalopathy, Chongqing Traditional Chinese Medicine Hospital, 
      Chongqing, China.
FAU - Ni, Yuehan
AU  - Ni Y
AUID- ORCID: 0000-0002-9696-7077
AD  - Department of Internal Medicine, Yuyao Traditional Chinese Medicine Hospital, 
      Yuyao, Zhejiang, China.
LA  - eng
GR  - 2022-ZX04/The fourth round of Key Disciplines Construction Plan of Municipal and 
      County Co-construction of TCM in Ningbo Integrated Traditional Chinese and 
      Western Medicine Nephropathy Science/
GR  - 2020YYB07/Yuyao City Health Science and Technology Plan Project/
PT  - Journal Article
DEP - 20230712
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 63LT81K70N (sinomenine)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Male
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - *Exosomes/pathology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Mice, Inbred C57BL
MH  - *Kidney Diseases/metabolism
MH  - Fibrosis
MH  - Autophagy
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
OTO - NOTNLM
OT  - autophagy
OT  - bone marrow mesenchymal stem cells
OT  - exosomes
OT  - miRNA
OT  - renal fibrosis
OT  - sinomenine
EDAT- 2023/07/12 13:07
MHDA- 2023/09/19 06:42
CRDT- 2023/07/12 09:53
PHST- 2023/06/12 00:00 [revised]
PHST- 2023/01/09 00:00 [received]
PHST- 2023/06/29 00:00 [accepted]
PHST- 2023/09/19 06:42 [medline]
PHST- 2023/07/12 13:07 [pubmed]
PHST- 2023/07/12 09:53 [entrez]
AID - 10.1002/tox.23890 [doi]
PST - ppublish
SO  - Environ Toxicol. 2023 Oct;38(10):2524-2537. doi: 10.1002/tox.23890. Epub 2023 Jul 
      12.