PMID- 37437580
OWN - NLM
STAT- MEDLINE
DCOM- 20230831
LR  - 20230912
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 18
IP  - 5
DP  - 2023 Aug 30
TI  - TiO(2)nanotubes promote osteogenic differentiation of human bone marrow stem 
      cells via epigenetic regulation ofRMRP/DLEU2/EZH2 pathway.
LID - 10.1088/1748-605X/ace6e9 [doi]
AB  - TiO(2)nanotubes (TNTs) significantly promote osteogenic differentiation and bone 
      regeneration of cells. Nevertheless, the biological processes by which they 
      promote osteogenesis are currently poorly understood. Long non-coding RNAs 
      (lncRNAs) are essential for controlling osteogenic differentiation of bone marrow 
      mesenchymal stem cells (BMSCs). Epigenetic chromatin modification is one of the 
      pathways in which lncRNAs regulate osteogenic differentiation. Here, we reported 
      that TNTs could upregulate lncRNARMRP, and inhibition of lncRNARMRPin human BMSCs 
      (hBMSCs) grown on TNTs could decrease runt-related transcription factor 2 
      (RUNX2), alkaline phosphatase, osteopontin, and osteocalcin (OCN) expression. 
      Furthermore, we discovered that inhibiting lncRNARMRPelevated the expression of 
      lncRNADLEU2, and lncRNADLEU2knockdown promoted osteogenic differentiation in 
      hBMSCs. RNA immunoprecipitation experiments showed that lncRNADLEU2could interact 
      with EZH2 to induce H3K27 methylation in the promoter regions of RUNX2 and OCN, 
      suppressing gene expression epigenetically. According to these results, 
      lncRNARMRPis upregulated by TNTs to promote osteogenic differentiation 
      throughDLEU2/EZH2-mediated epigenetic modifications.
CI  - (c) 2023 IOP Publishing Ltd.
FAU - Li, Shuangqin
AU  - Li S
AUID- ORCID: 0000-0002-2084-5815
AD  - Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong 
      University, Chengdu 610031, Sichuan, People's Republic of China.
FAU - Deng, Qing
AU  - Deng Q
AD  - Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong 
      University, Chengdu 610031, Sichuan, People's Republic of China.
FAU - Si, Qiqi
AU  - Si Q
AD  - School of Life and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 
      610031, People's Republic of China.
FAU - Li, JinSheng
AU  - Li J
AD  - Key Laboratory of Advanced Technologies of Materials Ministry of Education, 
      School of Materials Science and Engineering, Southwest Jiaotong University, 
      Chengdu 610031, People's Republic of China.
FAU - Zeng, Huanghe
AU  - Zeng H
AD  - School of Life and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 
      610031, People's Republic of China.
FAU - Chen, Song
AU  - Chen S
AD  - Department of Orthopedics of the General Hospital of Western Theater Command, 
      Chengdu, Sichuan 610086, People's Republic of China.
FAU - Guo, Tailin
AU  - Guo T
AD  - Institute of Biomedical Engineering, College of Medicine, Southwest Jiaotong 
      University, Chengdu 610031, Sichuan, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230830
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (MicroRNAs)
RN  - EC 2.1.1.43 (EZH2 protein, human)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
SB  - IM
MH  - Humans
MH  - Osteogenesis/physiology
MH  - Core Binding Factor Alpha 1 Subunit/genetics/metabolism
MH  - Epigenesis, Genetic
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Cell Differentiation/physiology
MH  - Bone Marrow Cells
MH  - *Nanotubes
MH  - Cells, Cultured
MH  - *MicroRNAs/metabolism
MH  - Enhancer of Zeste Homolog 2 Protein/genetics/metabolism
OTO - NOTNLM
OT  - DLEU2
OT  - RMRP
OT  - TiO2 nanotubes
OT  - epigenetics
OT  - osteogenic differentiation
EDAT- 2023/07/13 01:06
MHDA- 2023/08/31 06:42
CRDT- 2023/07/12 18:52
PHST- 2023/01/28 00:00 [received]
PHST- 2023/07/12 00:00 [accepted]
PHST- 2023/08/31 06:42 [medline]
PHST- 2023/07/13 01:06 [pubmed]
PHST- 2023/07/12 18:52 [entrez]
AID - 10.1088/1748-605X/ace6e9 [doi]
PST - epublish
SO  - Biomed Mater. 2023 Aug 30;18(5). doi: 10.1088/1748-605X/ace6e9.