PMID- 37438828
OWN - NLM
STAT- MEDLINE
DCOM- 20230717
LR  - 20230718
IS  - 0717-6287 (Electronic)
IS  - 0716-9760 (Print)
IS  - 0716-9760 (Linking)
VI  - 56
IP  - 1
DP  - 2023 Jul 13
TI  - Hyperbaric oxygen treatment increases intestinal stem cell proliferation through 
      the mTORC1/S6K1 signaling pathway in Mus musculus.
PG  - 41
LID - 10.1186/s40659-023-00444-3 [doi]
LID - 41
AB  - BACKGROUND: Hyperbaric oxygen treatment (HBOT) has been reported to modulate the 
      proliferation of neural and mesenchymal stem cell populations, but the molecular 
      mechanisms underlying these effects are not completely understood. In this study, 
      we aimed to assess HBOT somatic stem cell modulation by evaluating the role of 
      the mTOR complex 1 (mTORC1), a key regulator of cell metabolism whose activity is 
      modified depending on oxygen levels, as a potential mediator of HBOT in murine 
      intestinal stem cells (ISCs). RESULTS: We discovered that acute HBOT 
      synchronously increases the proliferation of ISCs without affecting the animal's 
      oxidative metabolism through activation of the mTORC1/S6K1 axis. mTORC1 
      inhibition by rapamycin administration for 20 days also increases ISCs 
      proliferation, generating a paradoxical response in mice intestines, and has been 
      proposed to mimic a partial starvation state. Interestingly, the combination of 
      HBOT and rapamycin does not have a synergic effect, possibly due to their 
      differential impact on the mTORC1/S6K1 axis. CONCLUSIONS: HBOT can induce an 
      increase in ISCs proliferation along with other cell populations within the crypt 
      through mTORC1/S6K1 modulation without altering the oxidative metabolism of the 
      animal's small intestine. These results shed light on the molecular mechanisms 
      underlying HBOT therapeutic action, laying the groundwork for future studies.
CI  - (c) 2023. The Author(s).
FAU - Casanova-Maldonado, Ignacio
AU  - Casanova-Maldonado I
AUID- ORCID: 0000-0001-9502-570X
AD  - Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, 
      Universidad de Chile, Las Encinas 3370, Milenio Building Floor 3, 7800024, 
      Santiago de Chile, Nunoa, Chile. Ignacio.casanova.m@gmail.com.
FAU - Arancibia, David
AU  - Arancibia D
AD  - Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, 
      Universidad de Chile, Las Encinas 3370, Milenio Building Floor 3, 7800024, 
      Santiago de Chile, Nunoa, Chile.
FAU - Lois, Pablo
AU  - Lois P
AD  - Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, 
      Universidad de Chile, Las Encinas 3370, Milenio Building Floor 3, 7800024, 
      Santiago de Chile, Nunoa, Chile.
AD  - Education Department, Faculty of Humanities, Universidad Mayor, Santiago de 
      Chile, Providencia, Chile.
FAU - Pena-Villalobos, Isaac
AU  - Pena-Villalobos I
AD  - Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, 
      Universidad de Chile, Las Encinas 3370, Milenio Building Floor 3, 7800024, 
      Santiago de Chile, Nunoa, Chile. isaac.pena@uchile.cl.
FAU - Palma, Veronica
AU  - Palma V
AD  - Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, 
      Universidad de Chile, Las Encinas 3370, Milenio Building Floor 3, 7800024, 
      Santiago de Chile, Nunoa, Chile. vpalma@uchile.cl.
LA  - eng
GR  - D09E1047/FONDEF/
GR  - 3180108/FONDECYT/
GR  - 1140697/FONDECYT/
GR  - 1110237/FONDECYT/
PT  - Journal Article
DEP - 20230713
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - S88TT14065 (Oxygen)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Cell Proliferation
MH  - *Hyperbaric Oxygenation
MH  - Intestines/cytology
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Oxygen
MH  - *Signal Transduction
MH  - Sirolimus/pharmacology
MH  - *Stem Cells/drug effects
PMC - PMC10339527
OTO - NOTNLM
OT  - Caloric restriction
OT  - Clarity
OT  - Hyperbaric oxygen treatment
OT  - Intestinal stem cells
OT  - Progenitor cells
OT  - Proliferation
OT  - Rapamycin
OT  - S6K1
OT  - mTORC1
COIS- The authors declare no competing financial interests.
EDAT- 2023/07/13 01:06
MHDA- 2023/07/14 13:08
PMCR- 2023/07/13
CRDT- 2023/07/12 23:51
PHST- 2023/01/31 00:00 [received]
PHST- 2023/06/05 00:00 [accepted]
PHST- 2023/07/14 13:08 [medline]
PHST- 2023/07/13 01:06 [pubmed]
PHST- 2023/07/12 23:51 [entrez]
PHST- 2023/07/13 00:00 [pmc-release]
AID - 10.1186/s40659-023-00444-3 [pii]
AID - 444 [pii]
AID - 10.1186/s40659-023-00444-3 [doi]
PST - epublish
SO  - Biol Res. 2023 Jul 13;56(1):41. doi: 10.1186/s40659-023-00444-3.