PMID- 37439906
OWN - NLM
STAT- MEDLINE
DCOM- 20240115
LR  - 20240115
IS  - 1439-6327 (Electronic)
IS  - 1439-6319 (Print)
IS  - 1439-6319 (Linking)
VI  - 124
IP  - 1
DP  - 2024 Jan
TI  - Combined effects of green tea supplementation and eccentric exercise on nuclear 
      factor erythroid 2-related factor 2 activity.
PG  - 245-256
LID - 10.1007/s00421-023-05271-8 [doi]
AB  - PURPOSE: This study investigated whether combining eccentric exercise and green 
      tea supplementation synergistically increased nuclear factor erythroid 2-related 
      factor 2 (NRF2) activity, a transcription factor responsible for coordinating 
      endogenous antioxidant expression. METHODS: In a double-blinded, randomized, 
      between-subjects design, 24 males (mean [SD]; 23 [3] years, 179.6 [6.1] cm, 78.8 
      [10.6] kg) performed 100 drop jumps following a 6 days supplementation period 
      with either green tea (poly)phenols (n = 12; 500 mg.d(-1)) or a placebo (n = 12; 
      inulin). NRF2/antioxidant response element (ARE) binding in peripheral blood 
      mononuclear cells (PBMCs), catalase (CAT) and glutathione reductase (GR) 
      activity, 8-hydroxy-2'-deoxyguanosine (8-OHdG) excretion, and differential 
      leukocyte counts were measured pre-, post-, 1 h and 24 h post-exercise. RESULTS: 
      Exercise did not increase NRF2/ARE binding (p = 0.12) (fold change vs rest: green 
      tea = [post] 0.78 +/- 0.45, [1 h] 1.17 +/- 0.54, [24 h] 1.06 +/- 0.56; placebo = [post] 
      1.40 +/- 1.50, [1 h] 2.98 +/- 3.70, [24 h] 1.04 +/- 0.45). Furthermore, CAT activity 
      (p = 0.12) and 8-OHdG excretion (p = 0.42) were unchanged in response to exercise 
      and were not augmented by green tea supplementation (p > 0.05 for all). Exercise 
      increased GR activity by 30% (p = 0.01), however no differences were found 
      between supplement groups (p = 0.51). Leukocyte and neutrophil concentrations 
      were only elevated post-exercise (p < 0.001 for all). CONCLUSION: Eccentric 
      exercise, either performed alone or in conjunction with green tea 
      supplementation, did not significantly increase NRF2 activity in PBMCs. TRIAL 
      REGISTRATION NUMBER: osf.io/kz37g (registered: 15/09/21).
CI  - (c) 2023. The Author(s).
FAU - Thorley, Josh
AU  - Thorley J
AUID- ORCID: 0009-0007-4450-7283
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK.
FAU - Thomas, Craig
AU  - Thomas C
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK.
FAU - Thon, Nicolas
AU  - Thon N
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK.
FAU - Nuttall, Hannah
AU  - Nuttall H
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK.
FAU - Martin, Neil R W
AU  - Martin NRW
AUID- ORCID: 0000-0002-1988-047X
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK.
FAU - Bishop, Nicolette
AU  - Bishop N
AUID- ORCID: 0000-0001-6221-3907
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK.
FAU - Bailey, Stephen J
AU  - Bailey SJ
AUID- ORCID: 0000-0003-0923-6362
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK.
FAU - Clifford, Tom
AU  - Clifford T
AUID- ORCID: 0000-0003-0484-2953
AD  - School of Sport, Exercise and Health Sciences, Loughborough University, 
      Loughborough, LE11 3TU, UK. t.clifford@lboro.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20230713
PL  - Germany
TA  - Eur J Appl Physiol
JT  - European journal of applied physiology
JID - 100954790
RN  - 0 (Tea)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Antioxidants)
SB  - IM
MH  - Male
MH  - Humans
MH  - *Tea
MH  - *NF-E2-Related Factor 2/metabolism
MH  - Leukocytes, Mononuclear
MH  - Antioxidants/pharmacology/metabolism
MH  - Dietary Supplements
MH  - Oxidative Stress/physiology
PMC - PMC10786739
OTO - NOTNLM
OT  - Antioxidants
OT  - Exercise
OT  - Oxidative stress
OT  - Polyphenols
OT  - Redox balance
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/13 12:31
MHDA- 2024/01/15 12:43
PMCR- 2023/07/13
CRDT- 2023/07/13 11:09
PHST- 2023/05/11 00:00 [received]
PHST- 2023/06/22 00:00 [accepted]
PHST- 2024/01/15 12:43 [medline]
PHST- 2023/07/13 12:31 [pubmed]
PHST- 2023/07/13 11:09 [entrez]
PHST- 2023/07/13 00:00 [pmc-release]
AID - 10.1007/s00421-023-05271-8 [pii]
AID - 5271 [pii]
AID - 10.1007/s00421-023-05271-8 [doi]
PST - ppublish
SO  - Eur J Appl Physiol. 2024 Jan;124(1):245-256. doi: 10.1007/s00421-023-05271-8. 
      Epub 2023 Jul 13.