PMID- 37443763
OWN - NLM
STAT- MEDLINE
DCOM- 20230717
LR  - 20240131
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 12
IP  - 13
DP  - 2023 Jun 27
TI  - Efficacy of Rectal Systemic Administration of Mesenchymal Stem Cells to Injury 
      Sites via the CXCL12/CXCR4 Axis to Promote Regeneration in a Rabbit Skeletal 
      Muscle Injury Model.
LID - 10.3390/cells12131729 [doi]
LID - 1729
AB  - Mesenchymal stem cells (MSCs) have been transplanted directly into lesions or 
      injected intravenously. The administration of MSCs using these delivery methods 
      requires specialized knowledge, techniques, and facilities. Here, we describe 
      intrarectal systemic administration of MSCs, a simple, non-invasive route for 
      homing to the injury sites to promote the regeneration of skeletal muscle 
      injuries. Using a cardiotoxin (CTX)-induced rabbit skeletal muscle injury model, 
      homing to the site of muscle injury was confirmed by intrarectal administration 
      of MSCs; the time required for homing after intrarectal administration was 
      approximately 5 days. In addition, the C-X-C chemokine ligand 12 (CXCL12)/C-X-C 
      chemokine receptor-4 (CXCR4) axis was found to be involved in the homing process. 
      Histopathological examinations showed that skeletal muscle regeneration was 
      promoted in the MSCs-administered group compared to the CTX-only group. Myosin 
      heavy polypeptide 3 (Myh3) expression, an indicator of early muscle regeneration, 
      was detected earlier in the intrarectal MSCs group compared to the CTX-only 
      group. These findings indicate that intrarectal administration of MSCs is 
      effective in homing to the injured area, where they promote injury repair. Since 
      intrarectal administration is a simple and non-invasive delivery route, these 
      findings may be valuable in future research on stem cell therapy.
FAU - Ichiseki, Toru
AU  - Ichiseki T
AD  - Department of Orthopaedic Surgery, Kanazawa Medical University, Daigaku 1-1, 
      Uchinada-machi, Kahoku 920-0293, Japan.
FAU - Shimasaki, Miyako
AU  - Shimasaki M
AD  - Department of Pathology 2, Kanazawa Medical University, Daigaku 1-1, 
      Uchinada-machi, Kahoku 920-0293, Japan.
FAU - Ueda, Shusuke
AU  - Ueda S
AUID- ORCID: 0000-0003-3385-032X
AD  - Department of Orthopaedic Surgery, Kanazawa Medical University, Daigaku 1-1, 
      Uchinada-machi, Kahoku 920-0293, Japan.
FAU - Hirata, Hiroaki
AU  - Hirata H
AD  - Department of Orthopaedic Surgery, Kanazawa Medical University, Daigaku 1-1, 
      Uchinada-machi, Kahoku 920-0293, Japan.
FAU - Souma, Daisuke
AU  - Souma D
AD  - Department of Orthopaedic Surgery, Kanazawa Medical University, Daigaku 1-1, 
      Uchinada-machi, Kahoku 920-0293, Japan.
FAU - Kawahara, Norio
AU  - Kawahara N
AD  - Department of Orthopaedic Surgery, Kanazawa Medical University, Daigaku 1-1, 
      Uchinada-machi, Kahoku 920-0293, Japan.
FAU - Ueda, Yoshimichi
AU  - Ueda Y
AD  - Department of Pathology, Keiju Medical Center, 94, Tomioka-machi, Nanao 926-0816, 
      Japan.
LA  - eng
GR  - 20K09513, 19K18510,18K07002,19K18511/The Ministry of Education, Culture, Sports, 
      Science, and Technology of Japan/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230627
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Rabbits
MH  - *Chemokine CXCL12/metabolism
MH  - Ligands
MH  - Muscle, Skeletal/metabolism
MH  - Peptides/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
PMC - PMC10340610
OTO - NOTNLM
OT  - C-X-C chemokine ligand 12 (CXCL12)/C-X-C chemokine receptor-4 (CXCR4) axis
OT  - cardiotoxin (CTX)
OT  - mesenchymal stem cells (MSCs)
OT  - myosin heavy polypeptide 3 (Myh3)
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/14 13:06
MHDA- 2023/07/17 06:42
PMCR- 2023/06/27
CRDT- 2023/07/14 01:02
PHST- 2023/03/20 00:00 [received]
PHST- 2023/06/22 00:00 [revised]
PHST- 2023/06/22 00:00 [accepted]
PHST- 2023/07/17 06:42 [medline]
PHST- 2023/07/14 13:06 [pubmed]
PHST- 2023/07/14 01:02 [entrez]
PHST- 2023/06/27 00:00 [pmc-release]
AID - cells12131729 [pii]
AID - cells-12-01729 [pii]
AID - 10.3390/cells12131729 [doi]
PST - epublish
SO  - Cells. 2023 Jun 27;12(13):1729. doi: 10.3390/cells12131729.