PMID- 37447299
OWN - NLM
STAT- MEDLINE
DCOM- 20230717
LR  - 20230718
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 15
IP  - 13
DP  - 2023 Jun 30
TI  - Piceatannol Protects PC-12 Cells against Oxidative Damage and Mitochondrial 
      Dysfunction by Inhibiting Autophagy via SIRT3 Pathway.
LID - 10.3390/nu15132973 [doi]
LID - 2973
AB  - Oxidative stress has been identified as a major cause of cellular injury in a 
      variety of neurodegenerative disorders. This study aimed to investigate the 
      cytoprotective effects of piceatannol on hydrogen peroxide (H(2)O(2))-induced 
      pheochromocytoma-12 (PC-12) cell damage and explore the underlying mechanisms. 
      Our findings indicated that piceatannol pre-treatment significantly attenuated 
      H(2)O(2)-induced PC-12 cell death. Furthermore, piceatannol effectively improved 
      mitochondrial content and mitochondrial function, including enhancing 
      mitochondrial reactive oxygen species (ROS) elimination capacity and increasing 
      mitochondrial transcription factor (TFAM), peroxisome-proliferator-activated 
      receptor-gamma coactivator-1alpha (PGC-1alpha) and mitochondria Complex IV expression. 
      Meanwhile, piceatannol treatment inhibited mitochondria-mediated autophagy as 
      demonstrated by restoring mitochondrial membrane potential, reducing 
      autophagosome formation and light chain 3B II/I (LC3B II/I) and autophagy-related 
      protein 5 (ATG5) expression level. The protein expression level of SIRT3 was 
      significantly increased by piceatannol in a concentration-dependent manner. 
      However, the cytoprotective effect of piceatannol was dramatically abolished by 
      sirtuin 3 (SIRT3) inhibitor, 3-(1H-1,2,3-Triazol-4-yl) pyridine (3-TYP), which 
      led to an exacerbated mitochondrial dysfunction and autophagy in PC-12 cells 
      under oxidative stress. In addition, the autophagy activator (rapamycin) 
      abrogated the protective effects of piceatannol on PC-12 cell death. These 
      findings demonstrated that piceatannol could alleviate PC-12 cell oxidative 
      damage and mitochondrial dysfunction by inhibiting autophagy via the SIRT3 
      pathway.
FAU - Liu, Jie
AU  - Liu J
AUID- ORCID: 0000-0003-0843-0358
AD  - Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing 
      Technology and Business University (BTBU), Beijing 100048, China.
AD  - China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology 
      and Business University (BTBU), Beijing 100048, China.
FAU - Mai, Peishi
AU  - Mai P
AD  - Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing 
      Technology and Business University (BTBU), Beijing 100048, China.
FAU - Yang, Zihui
AU  - Yang Z
AD  - Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing 
      Technology and Business University (BTBU), Beijing 100048, China.
FAU - Wang, Zongwei
AU  - Wang Z
AD  - Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing 
      Technology and Business University (BTBU), Beijing 100048, China.
FAU - Yang, Wei
AU  - Yang W
AD  - College of Basic Science, Tianjin Agricultural University, Tianjin 300392, China.
FAU - Wang, Ziyuan
AU  - Wang Z
AUID- ORCID: 0000-0002-7095-8527
AD  - Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing 
      Technology and Business University (BTBU), Beijing 100048, China.
AD  - China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology 
      and Business University (BTBU), Beijing 100048, China.
LA  - eng
GR  - BPHR202203044/Project of Cultivation for young top-notch Talents of Beijing 
      Municipal Institutions/
GR  - KM202110011004/General Project of Beijing Municipal Commission of Education/
GR  - 2021CXGC010807-03/Key R&D Program of Shandong Province/
PT  - Journal Article
DEP - 20230630
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - EC 3.5.1.- (Sirtuin 3)
RN  - 6KS3LS0D4F (3,3',4,5'-tetrahydroxystilbene)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - EC 3.5.1.- (SIRT3 protein, human)
SB  - IM
MH  - Humans
MH  - *Sirtuin 3/genetics/metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - *Pheochromocytoma/metabolism
MH  - Oxidative Stress
MH  - Mitochondria/metabolism
MH  - Autophagy
MH  - *Adrenal Gland Neoplasms/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 
      1-alpha/genetics/metabolism
PMC - PMC10346636
OTO - NOTNLM
OT  - SIRT3
OT  - autophagy
OT  - mitochondrial function
OT  - oxidative damage
OT  - piceatannol
COIS- The authors declare that there are no conflict of interest.
EDAT- 2023/07/14 13:06
MHDA- 2023/07/17 06:42
PMCR- 2023/06/30
CRDT- 2023/07/14 01:22
PHST- 2023/05/19 00:00 [received]
PHST- 2023/06/22 00:00 [revised]
PHST- 2023/06/25 00:00 [accepted]
PHST- 2023/07/17 06:42 [medline]
PHST- 2023/07/14 13:06 [pubmed]
PHST- 2023/07/14 01:22 [entrez]
PHST- 2023/06/30 00:00 [pmc-release]
AID - nu15132973 [pii]
AID - nutrients-15-02973 [pii]
AID - 10.3390/nu15132973 [doi]
PST - epublish
SO  - Nutrients. 2023 Jun 30;15(13):2973. doi: 10.3390/nu15132973.