PMID- 37448673
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230718
IS  - 2008-3289 (Print)
IS  - 2008-4447 (Electronic)
IS  - 2008-3289 (Linking)
VI  - 15
IP  - 3
DP  - 2023 Jun
TI  - Investigating the effect of MAP2K1 gene (MEK1) in MAPK pathway in the induction 
      of adult T-cell leukemia/lymphoma (ATLL).
PG  - 475-481
LID - 10.18502/ijm.v15i3.12910 [doi]
AB  - BACKGROUND AND OBJECTIVES: HTLV-1 is responsible for two important diseases, 
      HAM/TSP and ATLL. Approximately 10 to 20 million people are infected with HTLV-1 
      worldwide. Identifying altered genes in different cancers is crucial for finding 
      potential treatment strategies. One of the proteins of the RAS/MAPK signaling 
      pathway is MEK1, which is made from the MAP2K1 gene. The effects of the MAP2K1 
      gene on the MAPK signaling pathway are not yet fully elucidated. The current 
      study aims to determine the MAP2K1 gene mutations and the level of MAP2K1 gene 
      expression in ATLL patients compared to healthy individuals. MATERIALS AND 
      METHODS: Ten ATLL and 10 healthy control individuals were investigated in this 
      study. We used ELISA test to screen anti-HTLV-I antibodies and PCR for 
      confirmation of infection. Then, we extracted total RNA from fresh whole blood, 
      and cDNA was synthesized. The expression levels of the MAP2K1 gene were examined 
      by qRT-PCR, and to check possible mutations in the MAP2K1 gene; all samples were 
      sequenced and analyzed by BioEdite Software. RESULTS: MAP2K1 gene expression in 
      the ATLL group was significantly higher than in the healthy control (P=0.001). 
      The mutational sequencing analysis showed nucleotide 212 (S-->R) change and 
      identification mutations at different nucleotides that were entirely different 
      from the nucleotide mutations defined in the UniProt database. CONCLUSION: These 
      results could be a perspective in the prevention, prognosis, and targeted 
      treatment of diseases in which the MAP2K1 gene plays a vital role.
CI  - Copyright (c) 2023 The Authors. Published by Tehran University of Medical Sciences.
FAU - Hosseini, Parastoo
AU  - Hosseini P
AD  - Department of Virology, School of Public Health, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Rahimi Foroushani, Abbas
AU  - Rahimi Foroushani A
AD  - Department of Epidemiology and Biostatistics, School of Public Health, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Marjani, Arezoo
AU  - Marjani A
AD  - Department of Virology, School of Public Health, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Tavakoli, Mahnaz
AU  - Tavakoli M
AD  - Department of Medical Microbiology, School of Medical Science, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Amiri, Abdollah
AU  - Amiri A
AD  - Student Research Committee, School of Medicine, Alborz University of Medical 
      Sciences, Karaj, Iran.
FAU - Hosseini, Amin
AU  - Hosseini A
AD  - Department of Computer, Faculty of Engineering, Raja University, Qazvin, Iran.
FAU - Bahavar, Atefeh
AU  - Bahavar A
AD  - Department of Microbiology, School of Medicine, Golestan University of Medical 
      Sciences, Gorgan, Iran.
FAU - Mozhgani, Sayed-Hamidreza
AU  - Mozhgani SH
AD  - Department of Microbiology, School of Medicine, Alborz University of Medical 
      Sciences, Karaj, Iran.
FAU - Norouzi, Mehdi
AU  - Norouzi M
AD  - Department of Virology, School of Public Health, Tehran University of Medical 
      Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Microbiol
JT  - Iranian journal of microbiology
JID - 101518404
PMC - PMC10336286
OTO - NOTNLM
OT  - Adult T-cell leukemia/lymphoma
OT  - HTLV-I-associated myelopathy
OT  - Keywords: Human T-lymphotropic virus type 1
OT  - MAP kinase/ERK kinase
OT  - Mitogen-activated protein kinase kinase 1
OT  - Tropical spastic paraparesis
EDAT- 2023/07/14 13:06
MHDA- 2023/07/14 13:07
PMCR- 2023/06/01
CRDT- 2023/07/14 03:49
PHST- 2023/07/14 13:07 [medline]
PHST- 2023/07/14 13:06 [pubmed]
PHST- 2023/07/14 03:49 [entrez]
PHST- 2023/06/01 00:00 [pmc-release]
AID - IJM-15-475 [pii]
AID - 10.18502/ijm.v15i3.12910 [doi]
PST - ppublish
SO  - Iran J Microbiol. 2023 Jun;15(3):475-481. doi: 10.18502/ijm.v15i3.12910.