PMID- 37451224
OWN - NLM
STAT- MEDLINE
DCOM- 20230811
LR  - 20230811
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 229
DP  - 2023 Sep
TI  - Effect of osteoblasts on osteoclast differentiation and activity induced by 
      titanium with nanotopography.
PG  - 113448
LID - S0927-7765(23)00326-0 [pii]
LID - 10.1016/j.colsurfb.2023.113448 [doi]
AB  - Titanium with nanotopography (Ti Nano) favors osteoblast differentiation and 
      attenuates the osteoclast inhibitory effects on osteoblasts. Because the 
      interactions between nanotopography and osteoclasts are underexplored, the aims 
      of this study were to evaluate the effects of Ti Nano on osteoclast 
      differentiation and activity, and the influence of osteoblasts on osteoclast-Ti 
      Nano interaction. The discs were conditioned with a mixture of 10 N H(2)SO(4) and 
      30% aqueous H(2)O(2) to create Ti Nano and non-conditioned Ti discs were used as 
      control (Ti Control). Osteoclasts were cultured on Ti Control and Ti Nano in the 
      presence of osteoblasts in an indirect co-culture system. Also, osteoclasts were 
      cultured on polystyrene and calcium phosphate plates in conditioned media by 
      osteoblasts grown on Ti Control and Ti Nano. While Ti Control exhibited an 
      irregular and smooth surface, Ti Nano presented nanopores distributed throughout 
      the whole surface. Additionally, anisotropy was higher on Ti Nano than Ti 
      Control. Nanotopography favored the gene expression of osteoclast markers but 
      inhibited osteoclast differentiation and activity, and the presence of 
      osteoblasts enhanced the effects of Ti Nano on osteoclasts. Such findings were 
      mimicked by conditioned medium of osteoblasts cultured on Ti Nano, which reduced 
      the osteoclast differentiation and activity. In conclusion, our results indicated 
      that nanotopography regulates osteoblast-osteoclast crosstalk and further 
      investigations should focus the impact of these bone cell interactions on Ti 
      osseointegration.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Bighetti-Trevisan, Rayana Longo
AU  - Bighetti-Trevisan RL
AD  - Bone Research Lab, School of Dentistry of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto 14040-904, SP, Brazil.
FAU - Ferraz, Emanuela Prado
AU  - Ferraz EP
AD  - Bone Research Lab, School of Dentistry of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto 14040-904, SP, Brazil.
FAU - Silva, Marcelle Beathriz Fernandes
AU  - Silva MBF
AD  - Bone Research Lab, School of Dentistry of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto 14040-904, SP, Brazil.
FAU - Zatta, Guilherme Crepi
AU  - Zatta GC
AD  - Bone Research Lab, School of Dentistry of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto 14040-904, SP, Brazil.
FAU - de Almeida, Marcelo Barros
AU  - de Almeida MB
AD  - School of Electrical Engineering, Federal University of Uberlandia, Uberlandia, 
      38408-100 MG, Brazil.
FAU - Rosa, Adalberto Luiz
AU  - Rosa AL
AD  - Bone Research Lab, School of Dentistry of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto 14040-904, SP, Brazil.
FAU - Beloti, Marcio Mateus
AU  - Beloti MM
AD  - Bone Research Lab, School of Dentistry of Ribeirao Preto, University of Sao 
      Paulo, Ribeirao Preto 14040-904, SP, Brazil. Electronic address: mmbeloti@usp.br.
LA  - eng
PT  - Journal Article
DEP - 20230710
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - D1JT611TNE (Titanium)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - *Osteoclasts
MH  - *Titanium/pharmacology/metabolism
MH  - Hydrogen Peroxide/pharmacology
MH  - Osteoblasts
MH  - Cell Differentiation
OTO - NOTNLM
OT  - Bone
OT  - Cell culture
OT  - Implant
OT  - Nanosurface
OT  - Osteoblast
OT  - Osteoclast
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/07/15 10:42
MHDA- 2023/08/11 06:42
CRDT- 2023/07/14 18:10
PHST- 2023/05/12 00:00 [received]
PHST- 2023/07/03 00:00 [revised]
PHST- 2023/07/08 00:00 [accepted]
PHST- 2023/08/11 06:42 [medline]
PHST- 2023/07/15 10:42 [pubmed]
PHST- 2023/07/14 18:10 [entrez]
AID - S0927-7765(23)00326-0 [pii]
AID - 10.1016/j.colsurfb.2023.113448 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2023 Sep;229:113448. doi: 
      10.1016/j.colsurfb.2023.113448. Epub 2023 Jul 10.