PMID- 37451381 OWN - NLM STAT- MEDLINE DCOM- 20230922 LR - 20230922 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 248 DP - 2023 Sep 1 TI - Chitosan-encapsulated nickel oxide, tin dioxide, and farnesol nanoparticles: Antimicrobial and anticancer properties in breast cancer cells. PG - 125799 LID - S0141-8130(23)02694-6 [pii] LID - 10.1016/j.ijbiomac.2023.125799 [doi] AB - Breast cancer is the most frequent cancer in women; however, it is curable in most cases (up to 80 %) when detected and treated at an early non-metastatic stage. Nanotechnology has led to the development of potential chemotherapeutic techniques, particularly for tumor treatment. Nanotechnology has therapeutic and pharmaceutical applications. Chitosan, a natural polymer derived from chitin, has been extensively studied for its potential applications in a wide range of fields. This includes medicine for its anticancer properties. In the present study, Chitosan-encapsulated-NiO-TiO2-Farnesol hybrid nanomaterials (CNTF HNMs) were synthesized and characterized using several techniques, including electron microscopy (TEM, FE-SEM), spectroscopy (UV-visible [UV-Vis], Fourier Transform Infrared [FT-IR] spectroscopy, and photoluminescence [PL]), energy-dispersive X-ray spectroscopy (EDX) composition analysis, X-ray diffraction, and dynamic light scattering (DLS) analyses. With an estimated average crystallite size of 34.8 nm, the face-cantered cubic crystalline structure of the CNTF HNMs is identified. Cell viability assay by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DAPI (4',6-diamidino-2-phenylindole) staining, dual AO/EtBr (Acridine Orange/ Ethidium bromide), JC-1 (5,5,6,6'-tetrachloro-1,1',3,3' tetraethylbenzimi-dazoylcarbocyanine iodide), DCFH-DA (Dichloro-dihydro-fluorescein diacetate), Annexin V-FITC (Fluorescein isothiocyanate) /PI (Propidium Iodide), and cell cycle study was used to assess the ability of nanoparticles (NPs) to kill MDA-MB-231 cells. The CNTF HNMs had high antibacterial effectiveness against multi-drug resistant extended-spectrum beta-lactamases (ESBL)-producing gram-negative bacterial pathogens and reference strains. The findings suggest that NPs increased the number of reactive oxygen species (ROS), changed the Deltapsim, and initiated apoptosis. There is enormous potential for CNTF HNMs as both antibacterial and anticancer agents. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - Mickymaray, Suresh AU - Mickymaray S AD - Department of Biology, College of Science- Al-Zulfi, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia; Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India. Electronic address: s.maray@mu.edu.sa. FAU - Al Aboody, Mohammed Saleh AU - Al Aboody MS AD - Department of Biology, College of Science- Al-Zulfi, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia. Electronic address: m.alaboudi@mu.edu.sa. FAU - Eraqi, Mostafa M AU - Eraqi MM AD - Department of Biology, College of Science- Al-Zulfi, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia; Microbiology and Immunology Department, Veterinary Research Institute, National Research Centre, Dokki, Giza 12622, Egypt. Electronic address: m.eraqi@mu.edu.sa. FAU - Alhoqail, Wardha A AU - Alhoqail WA AD - Department of Biology, College of Education, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia. Electronic address: w.alhoqail@mu.edu.sa. FAU - Alothaim, Abdulaziz S AU - Alothaim AS AD - Department of Biology, College of Science- Al-Zulfi, Majmaah University, Majmaah 11952, Riyadh Region, Saudi Arabia. Electronic address: a.alothaim@mu.edu.sa. FAU - Suresh, Kaviya AU - Suresh K AD - Department of Pharmaceutics, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600 116, India. FAU - Arulselvan, Palanisamy AU - Arulselvan P AD - Muthayammal Centre for Advanced Research, Muthayammal College of Arts and Science, Rasipuram, Namakkal, Tamil Nadu 637408, India; Scigen Research and Innovation Pvt. Ltd., Periyar Technology Business Incubator, Thanjavur, Tamil Nadu, India. LA - eng PT - Journal Article DEP - 20230712 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - KM7N50LOS6 (stannic oxide) RN - 4602-84-0 (Farnesol) RN - 9012-76-4 (Chitosan) RN - C3574QBZ3Y (nickel monoxide) RN - 0 (Ciliary Neurotrophic Factor) RN - 0 (Anti-Infective Agents) RN - 0 (Anti-Bacterial Agents) SB - IM MH - Female MH - Humans MH - Farnesol MH - *Chitosan/pharmacology MH - Cell Line, Tumor MH - *Breast Neoplasms/drug therapy/pathology MH - Spectroscopy, Fourier Transform Infrared MH - Ciliary Neurotrophic Factor MH - *Metal Nanoparticles/chemistry MH - *Anti-Infective Agents MH - Anti-Bacterial Agents/chemistry OTO - NOTNLM OT - Antibacterial OT - Breast cancer OT - CNTF HNMs OT - Farnesol OT - MDA-MB-231 OT - Nickel oxide OT - Tin dioxide COIS- Declaration of competing interest Authors declare no conflicts of interest. EDAT- 2023/07/15 10:42 MHDA- 2023/09/22 06:42 CRDT- 2023/07/14 19:23 PHST- 2023/04/10 00:00 [received] PHST- 2023/07/04 00:00 [revised] PHST- 2023/07/10 00:00 [accepted] PHST- 2023/09/22 06:42 [medline] PHST- 2023/07/15 10:42 [pubmed] PHST- 2023/07/14 19:23 [entrez] AID - S0141-8130(23)02694-6 [pii] AID - 10.1016/j.ijbiomac.2023.125799 [doi] PST - ppublish SO - Int J Biol Macromol. 2023 Sep 1;248:125799. doi: 10.1016/j.ijbiomac.2023.125799. Epub 2023 Jul 12.