PMID- 37453957
OWN - NLM
STAT- MEDLINE
DCOM- 20230717
LR  - 20230918
IS  - 1438-7948 (Electronic)
IS  - 1438-793X (Linking)
VI  - 23
IP  - 3
DP  - 2023 Jul 15
TI  - Identification of genes governing resistance to PCN (Globodera rostochiensis) 
      through transcriptome analysis in Solanum tuberosum.
PG  - 242
LID - 10.1007/s10142-023-01164-3 [doi]
AB  - Potato cyst nematodes (PCNs) are major pests worldwide that affect potato 
      production. The molecular changes happening in the roots upon PCN infection are 
      still unknown. Identification of transcripts and genes governing PCN resistance 
      will help in the development of resistant varieties. Hence, differential gene 
      expression of compatible (Kufri Jyoti) and incompatible (JEX/A-267) potato 
      genotypes was studied before (0 DAI) and after (10 DAI) inoculation of Globodera 
      rostochiensis J2s through RNA sequencing (RNA-Seq). Total sequencing reads 
      generated ranged between 33 and 37 million per sample, with a read mapping of 
      48-84% to the potato reference genome. In the infected roots of the resistant 
      genotype JEX/A-267, 516 genes were downregulated, and 566 were upregulated. In 
      comparison, in the susceptible genotype Kufri Jyoti, 316 and 554 genes were 
      downregulated and upregulated, respectively. Genes encoding cell wall proteins, 
      zinc finger protein, WRKY transcription factors, MYB transcription factors, 
      disease resistance proteins, and pathogenesis-related proteins were found to be 
      majorly involved in the incompatible reaction after PCN infection in the 
      resistant genotype, JEX/A-267. Furthermore, RNA-Seq results were validated 
      through quantitative real-time PCR (qRT-PCR), and it was observed that ATP, 
      FLAVO, CYTO, and GP genes were upregulated at 5 DAI, which was subsequently 
      downregulated at 10 DAI. The genes encoding ATP, FLAVO, LBR, and GP were present 
      in > 1.5 fold before infection in JEX-A/267 and upregulated 7.9- to 27.6-fold 
      after 5 DAI; subsequently, most of these genes were downregulated to 0.9- to 
      2.8-fold, except LBR, which was again upregulated to 44.4-fold at 10 DAI.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Bairwa, Aarti
AU  - Bairwa A
AUID- ORCID: 0000-0003-4086-2433
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Sood, Salej
AU  - Sood S
AUID- ORCID: 0000-0002-3163-7501
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India. salej.sood@icar.gov.in.
FAU - Bhardwaj, Vinay
AU  - Bhardwaj V
AUID- ORCID: 0000-0002-3476-1444
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India. vinay.bhardwaj@icar.gov.in.
FAU - Rawat, Shashi
AU  - Rawat S
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Tamanna, Tamanna
AU  - Tamanna T
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Siddappa, Sundaresha
AU  - Siddappa S
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Venkatasalam, E P
AU  - Venkatasalam EP
AD  - ICAR-Central Potato Research Station, Muthorai, 643004, The Nilgiris, 
      Udhagamandalam, Tamil Nadu, India.
FAU - Dipta, Bhawna
AU  - Dipta B
AUID- ORCID: 0000-0001-7419-7414
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Sharma, Ashwani K
AU  - Sharma AK
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Kumar, Ashwani
AU  - Kumar A
AUID- ORCID: 0000-0002-8995-0074
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Singh, Baljeet
AU  - Singh B
AUID- ORCID: 0000-0001-7981-6624
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Mhatre, Priyank H
AU  - Mhatre PH
AUID- ORCID: 0000-0002-5205-8170
AD  - ICAR-Central Potato Research Station, Muthorai, 643004, The Nilgiris, 
      Udhagamandalam, Tamil Nadu, India.
FAU - Sharma, Sanjeev
AU  - Sharma S
AUID- ORCID: 0000-0002-0446-2304
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
FAU - Kumar, Vinod
AU  - Kumar V
AD  - ICAR-Central Potato Research Institute, Bemloe, 171001, Shimla, Himachal Pradesh, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20230715
PL  - Germany
TA  - Funct Integr Genomics
JT  - Functional & integrative genomics
JID - 100939343
RN  - 0 (Transcription Factors)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Animals
MH  - *Solanum tuberosum/genetics
MH  - Gene Expression Profiling/methods
MH  - *Tylenchoidea
MH  - Transcription Factors/genetics
MH  - Adenosine Triphosphate
OTO - NOTNLM
OT  - Gene Expression
OT  - Globodera rostochiensis
OT  - PCN Resistance
OT  - Potatoes
OT  - RNA Sequencing
EDAT- 2023/07/16 01:06
MHDA- 2023/07/17 06:42
CRDT- 2023/07/15 23:04
PHST- 2022/12/04 00:00 [received]
PHST- 2023/06/30 00:00 [accepted]
PHST- 2023/06/15 00:00 [revised]
PHST- 2023/07/17 06:42 [medline]
PHST- 2023/07/16 01:06 [pubmed]
PHST- 2023/07/15 23:04 [entrez]
AID - 10.1007/s10142-023-01164-3 [pii]
AID - 10.1007/s10142-023-01164-3 [doi]
PST - epublish
SO  - Funct Integr Genomics. 2023 Jul 15;23(3):242. doi: 10.1007/s10142-023-01164-3.