PMID- 37454456
OWN - NLM
STAT- MEDLINE
DCOM- 20230809
LR  - 20230809
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1705
DP  - 2023 Aug 30
TI  - Magnetic molecularly-imprinted microspheres with a core-shell structure for the 
      extraction of catalpol from both Rehmannia glutinosa Libosch and biological 
      samples.
PG  - 464183
LID - S0021-9673(23)00409-0 [pii]
LID - 10.1016/j.chroma.2023.464183 [doi]
AB  - We report on the successful development of a magnetic molecularly imprinted 
      polymer (MMIP) for the separation and detection of catalpol in Rehmannia 
      glutinosa extract and biological samples. Catalpol was used as the template 
      molecule for the MMIPs, which were synthesized using the reverse prediction 
      approach and differential UV-visible spectra to optimize the synthesis 
      conditions. The resulting MMIPs were characterized using techniques including 
      scanning electron microscopy, transmission electron microscopy, Fourier transform 
      infrared spectroscopy, and vibrating sample magnetometry, which confirmed their 
      excellent nuclear shell construction and simple rapid magnetic separation. Static 
      and dynamic adsorption experiments were performed to assess the sensitivity, 
      repeatability, and adsorption capacity of the MMIPs. Adsorption capacity was 
      found to be high (59.09 microg/mg). The MMIPs also exhibited strong identification 
      specificity for catalpol analogs (IF = 2.41). Magnetic solid phase extraction 
      (MSPE) was optimized to establish an MSPE-HPLC approach based on the MMIPs. Under 
      ideal conditions, the new method showed excellent determination coefficient 
      (R(2) > 0.99) in the ranges of 0.1-1.0 mg/mL and 0.03-0.18 mg/mL for R. glutinosa 
      extract and biological material, respectively, with limits of detection of 
      5.4 ng/mL and 1.7 ng/mL. The novelty of this research lies in the preparation of 
      magnetic surface MMIPs using a magnetic carrier. These MMIPs exhibit excellent 
      magnetic properties and possess specific adsorption capability for catalpol, 
      enabling rapid separation. They offer advantages such as low cost, high 
      specificity, chemical stability, physical robustness, and recyclability 
      (97.09%-106.53%, 91.32%-100.99%). The application of these polymers in the 
      pretreatment method for determining the catalpol content in R. glutinosa extract 
      and biological samples enhances the extraction efficiency and detection accuracy 
      for catalpol. It eliminates interference and influences from other components in 
      catalpol determination while exhibiting high sensitivity, good enrichment 
      effects, excellent reusability, and a high recovery rate.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Qin, Bing
AU  - Qin B
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China; School of 
      Pharmaceutical Sciences, Jiamusi University, Jiamusi, China.
FAU - Yu, Xiaoyan
AU  - Yu X
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China.
FAU - Gao, Mingyuan
AU  - Gao M
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China.
FAU - Zhang, Yanyan
AU  - Zhang Y
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China.
FAU - Wang, Chunling
AU  - Wang C
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China.
FAU - Wan, Hongle
AU  - Wan H
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China; School of 
      Pharmaceutical Sciences, Jiamusi University, Jiamusi, China.
FAU - Li, Li
AU  - Li L
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China.
FAU - Zhang, Muxin
AU  - Zhang M
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China.
FAU - Zhang, Mengyuan
AU  - Zhang M
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China.
FAU - Zhang, Weidong
AU  - Zhang W
AD  - School of Pharmaceutical Sciences, Jilin University, Changchun, China. Electronic 
      address: weidongzhang@jlu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230705
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 2415-24-9 (catalpol)
SB  - IM
MH  - *Rehmannia
MH  - *Molecular Imprinting/methods
MH  - Microspheres
MH  - Adsorption
MH  - Magnetic Phenomena
MH  - Solid Phase Extraction/methods
MH  - Chromatography, High Pressure Liquid
OTO - NOTNLM
OT  - Catalpol
OT  - Magnetic solid-phase extraction
OT  - Molecularly imprinted polymers
OT  - Rehmannia glutinosa
COIS- Declaration of Competing Interest We declare that we have no financial and 
      personal relationships with other people or organizations that can 
      inappropriately influence our work. There is no professional or other personal 
      interest of any nature or kind in any product, service and/or company that could 
      be construed as influencing the position presented in, or the review of, the 
      manuscript entitled.
EDAT- 2023/07/16 19:16
MHDA- 2023/08/09 06:43
CRDT- 2023/07/16 18:02
PHST- 2023/03/23 00:00 [received]
PHST- 2023/06/27 00:00 [revised]
PHST- 2023/06/27 00:00 [accepted]
PHST- 2023/08/09 06:43 [medline]
PHST- 2023/07/16 19:16 [pubmed]
PHST- 2023/07/16 18:02 [entrez]
AID - S0021-9673(23)00409-0 [pii]
AID - 10.1016/j.chroma.2023.464183 [doi]
PST - ppublish
SO  - J Chromatogr A. 2023 Aug 30;1705:464183. doi: 10.1016/j.chroma.2023.464183. Epub 
      2023 Jul 5.