PMID- 37454810
OWN - NLM
STAT- MEDLINE
DCOM- 20231026
LR  - 20231112
IS  - 1995-820X (Electronic)
IS  - 1674-0769 (Print)
IS  - 1995-820X (Linking)
VI  - 38
IP  - 5
DP  - 2023 Oct
TI  - miR-204 suppresses porcine reproductive and respiratory syndrome virus (PRRSV) 
      replication via inhibiting LC3B-mediated autophagy.
PG  - 690-698
LID - S1995-820X(23)00084-6 [pii]
LID - 10.1016/j.virs.2023.07.004 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) 
      has been regarded as a persistent challenge for the swine farms worldwide. 
      microRNAs (miRNAs) play key roles in regulating almost every important biological 
      process, including virus-host interaction. In this study, we found that miR-204 
      was highly expressed in cells that were not permissive to PRRSV infection 
      compared with cells susceptible to PRRSV infection. Subsequently, we demonstrated 
      that overexpression of miR-204 significantly inhibited PRRSV replication in 
      porcine alveolar macrophages (PAMs). Through bioinformatic analysis, we found 
      that there existed a potential binding site of miR-204 on the 3'UTR of 
      microtubule associated protein 1 light chain 3B (MAP1LC3B, LC3B), a hallmark of 
      autophagy. Applying experiments including luciferase reporter assay and UV 
      cross-linking and immunoprecipitation (CLIP) assay, we demonstrated that miR-204 
      directly targeted LC3B, thereby downregulating autophagy. Meanwhile, we 
      investigated the interplay between autophagy and PRRSV replication in PAMs, 
      confirming that PRRSV infection induces autophagy, which in turn facilitates 
      viral replication. Overall, we verify that miR-204 suppresses PRRSV replication 
      via inhibiting LC3B-mediated autophagy in PAMs. These findings will provide a 
      novel potential approach for us to develop antiviral therapeutic agents and 
      controlling measures for future PRRSV outbreaks.
CI  - Copyright (c) 2023 The Authors. Publishing services by Elsevier B.V. All rights 
      reserved.
FAU - Yao, Yao
AU  - Yao Y
AD  - State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for 
      Molecular Design Breeding, Ministry of Agriculture Key Laboratory of Soil 
      Microbiology, and Department of Microbiology and Immunology, College of 
      Biological Sciences, China Agricultural University, Beijing, 100193, China.
FAU - Li, Sihan
AU  - Li S
AD  - State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for 
      Molecular Design Breeding, Ministry of Agriculture Key Laboratory of Soil 
      Microbiology, and Department of Microbiology and Immunology, College of 
      Biological Sciences, China Agricultural University, Beijing, 100193, China.
FAU - Zhu, Yingqi
AU  - Zhu Y
AD  - State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for 
      Molecular Design Breeding, Ministry of Agriculture Key Laboratory of Soil 
      Microbiology, and Department of Microbiology and Immunology, College of 
      Biological Sciences, China Agricultural University, Beijing, 100193, China.
FAU - Xu, Yangyang
AU  - Xu Y
AD  - State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for 
      Molecular Design Breeding, Ministry of Agriculture Key Laboratory of Soil 
      Microbiology, and Department of Microbiology and Immunology, College of 
      Biological Sciences, China Agricultural University, Beijing, 100193, China.
FAU - Hao, Siyuan
AU  - Hao S
AD  - State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for 
      Molecular Design Breeding, Ministry of Agriculture Key Laboratory of Soil 
      Microbiology, and Department of Microbiology and Immunology, College of 
      Biological Sciences, China Agricultural University, Beijing, 100193, China.
FAU - Guo, Shuyuan
AU  - Guo S
AD  - State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for 
      Molecular Design Breeding, Ministry of Agriculture Key Laboratory of Soil 
      Microbiology, and Department of Microbiology and Immunology, College of 
      Biological Sciences, China Agricultural University, Beijing, 100193, China.
FAU - Feng, Wen-Hai
AU  - Feng WH
AD  - State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for 
      Molecular Design Breeding, Ministry of Agriculture Key Laboratory of Soil 
      Microbiology, and Department of Microbiology and Immunology, College of 
      Biological Sciences, China Agricultural University, Beijing, 100193, China. 
      Electronic address: whfeng@cau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230716
PL  - Netherlands
TA  - Virol Sin
JT  - Virologica Sinica
JID - 101514185
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Swine
MH  - Animals
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *Porcine Reproductive and Respiratory Syndrome/genetics
MH  - Cell Line
MH  - *MicroRNAs/genetics/metabolism
MH  - Virus Replication
MH  - Autophagy/genetics
PMC - PMC10590697
OTO - NOTNLM
OT  - Autophagy
OT  - LC3B
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - miR-204
COIS- Conflict of interest Prof. Wenhai Feng is an Editorial Board member for 
      Virologica Sinica and was not involved in the editorial review or the decision to 
      publish this article. The authors declare that they have no conflict of interest.
EDAT- 2023/07/17 00:42
MHDA- 2023/10/26 06:42
PMCR- 2023/07/16
CRDT- 2023/07/16 19:25
PHST- 2023/04/07 00:00 [received]
PHST- 2023/07/07 00:00 [accepted]
PHST- 2023/10/26 06:42 [medline]
PHST- 2023/07/17 00:42 [pubmed]
PHST- 2023/07/16 19:25 [entrez]
PHST- 2023/07/16 00:00 [pmc-release]
AID - S1995-820X(23)00084-6 [pii]
AID - 10.1016/j.virs.2023.07.004 [doi]
PST - ppublish
SO  - Virol Sin. 2023 Oct;38(5):690-698. doi: 10.1016/j.virs.2023.07.004. Epub 2023 Jul 
      16.