PMID- 3745810
OWN - NLM
STAT- MEDLINE
DCOM- 19861020
LR  - 20190820
IS  - 0022-1422 (Print)
IS  - 0022-1422 (Linking)
VI  - 41
IP  - 5
DP  - 1986 Sep
TI  - Age-related decline in ligand-induced actin polymerization in human leukocytes 
      and platelets.
PG  - 561-6
AB  - The change in monomeric actin was measured in human polymorphonuclear leukocytes 
      (PMN) and platelets, using the DNase I inhibition assay, after stimulation with 
      various agents. PMN were stimulated with the chemotactic peptide 
      n-formyl-methionyl-leucyl-phenylalanine (FMLP) in 199 donors, the tumor promoter 
      phorbol myristate acetate in 112 donors, and the hormones tri-iodothyronine and 
      phenylephrine, in 118 donors. The donors ranged in age from 20 to 89 years. A 
      decrease in ligand-induced actin polymerization was detected in PMN obtained from 
      the older population, on stimulation with FMLP (p = .014) and PMA (p = .0038). In 
      platelets stimulated with thrombin (163 donors) a significant decrease in actin 
      polymerization also was noted in the older group (p = .017). In addition, in 
      platelets actin polymerization was less in women compared with men (p = .042). 
      Because actin polymerization is an event that might have an important role in 
      cellular activation, the age-related decline in the physiological functions might 
      be related to a decrease in ligand-induced actin polymerization.
FAU - Rao, K M
AU  - Rao KM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Gerontol
JT  - Journal of gerontology
JID - 0374762
RN  - 0 (Actins)
RN  - 0 (Ligands)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Actins/*blood
MH  - Adult
MH  - Aged
MH  - *Aging
MH  - Blood Platelets/*drug effects
MH  - Female
MH  - Humans
MH  - In Vitro Techniques
MH  - Ligands
MH  - Male
MH  - Middle Aged
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*drug effects
MH  - Phenylephrine/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Triiodothyronine/pharmacology
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
AID - 10.1093/geronj/41.5.561 [doi]
PST - ppublish
SO  - J Gerontol. 1986 Sep;41(5):561-6. doi: 10.1093/geronj/41.5.561.