PMID- 37460031
OWN - NLM
STAT- MEDLINE
DCOM- 20230918
LR  - 20230918
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 318
IP  - Pt A
DP  - 2024 Jan 10
TI  - Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on 
      ovalbumin-induced asthma mice.
PG  - 116887
LID - S0378-8741(23)00755-9 [pii]
LID - 10.1016/j.jep.2023.116887 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: More than 300 million people worldwide suffer 
      from asthma, a chronic respiratory inflammatory disease. Total alkaloids (TA) 
      were extracted from the ethnic medicinal plant Alstonia solaris (L.) R.Br., which 
      is used to treat respiratory diseases. They may be effective drugs for treating 
      asthma, but research is still needed to determine their effectiveness and 
      mechanism in treating asthma. AIM OF THE STUDY: To further understand TA's role 
      in the treatment of asthma and to support the phase II trial of the drug. 
      MATERIALS AND METHODS: In this study, we investigated the effects of TA in a 
      mouse asthma model produced by Ovalbumin (OVA). H&E and PAS staining were used to 
      observe the histopathological features of lung. airway hyperresponsiveness (AHR) 
      was detected by ventilator; The expression of interleukin (IL)-33, suppression of 
      tumorigenicity 2 (ST2) and E-cadherin in the lungs was evaluated by IHC. The 
      concentrations of Mucin5AC (MUC5AC), eotaxin, IL-4, IL-5, IL-9, IL-13, interferon 
      (IFN)-gamma, IL-6, IL-8, IL-17A, IL-33, IL-25, thymic stromal lymphopoietin (TSLP), 
      monocyte chemoattractant protein 1 (MCP-1), leukotriene (LT) B(4), LTC(4), 
      LTD(4), LTE(4) in bronchoalveolar lavage fluid (BALF) and total IgE (tIgE), 
      OVA-Specific IgE (OVA-IgE) in serum were measured by ELISA. ILC2s and eosinophils 
      were detected in lung tissue by flow cytometry. The gene expression levels of 
      IL-33 and ST2 were detected by RT-qPCR. RESULTS: Administration of TA reduced 
      pulmonary inflammatory symptoms, MUC5AC production in the BALF, and AHR. At the 
      same time, TA inhibited eotaxin production and eosinophil recruitment. Moreover, 
      TA significantly decreased Th2 and Th17 cytokines and increased Th1 cytokines, 
      contributing to restore the balance between Th1 and Th2 and Th17 cytokines. TA 
      may reduce ILC2s numbers by inhibiting IL-33, IL-25, and TSLP levels in BALF and 
      IL-33/ST2 signaling in lung tissue. Finally, TA decreased tIgE, OVA-IgE, and 
      MCP-1 levels and subsequently inhibited mast cell activation and leukotriene 
      release. CONCLUSIONS: These findings imply that TA may be an effective 
      immunoregulatory medication for the management and prevention of asthma.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Tong, Xiaoyun
AU  - Tong X
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China; The 
      First Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming, 
      650021, China.
FAU - Zhao, Yunli
AU  - Zhao Y
AD  - Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education 
      and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of 
      Chemical Science and Technology, Yunnan University, Kunming, 650500, China.
FAU - Fu, Rongbing
AU  - Fu R
AD  - School of Pharmacy, Youjiang Medical University for Nationalities, Baise, 533000, 
      China.
FAU - Hu, Min
AU  - Hu M
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
FAU - Zhang, Qiushi
AU  - Zhang Q
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
FAU - Wu, Xiangnong
AU  - Wu X
AD  - The First Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming, 
      650021, China.
FAU - Qu, Lu
AU  - Qu L
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
FAU - Li, Baojing
AU  - Li B
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
FAU - Nie, Jian
AU  - Nie J
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
FAU - Hu, Chunyan
AU  - Hu C
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
FAU - Yu, Xiaoling
AU  - Yu X
AD  - The Third Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming, 
      650021, China.
FAU - Xie, Yuhuan
AU  - Xie Y
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China.
FAU - Luo, Xiaodong
AU  - Luo X
AD  - Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education 
      and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of 
      Chemical Science and Technology, Yunnan University, Kunming, 650500, China; State 
      Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China. 
      Electronic address: xdluo@mail.kib.ac.cn.
FAU - Huang, Feng
AU  - Huang F
AD  - School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal 
      Utilization, Yunnan University of Chinese Medicine, Kunming, 650500, China. 
      Electronic address: 14030@ynutcm.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230717
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 9006-59-1 (Ovalbumin)
RN  - 0 (Interleukin-33)
RN  - 0 (Interleukin-1 Receptor-Like 1 Protein)
RN  - 0 (Cytokines)
RN  - 0 (Alkaloids)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Leukotrienes)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Alstonia
MH  - Ovalbumin/pharmacology
MH  - Interleukin-33/metabolism
MH  - Interleukin-1 Receptor-Like 1 Protein/metabolism
MH  - Immunity, Innate
MH  - *Asthma/chemically induced/drug therapy/genetics
MH  - Lung
MH  - Cytokines/metabolism
MH  - *Alkaloids/pharmacology/therapeutic use/metabolism
MH  - Bronchoalveolar Lavage Fluid
MH  - Immunoglobulin E
MH  - Th17 Cells
MH  - Leukotrienes/metabolism/pharmacology/therapeutic use
MH  - Mice, Inbred BALB C
MH  - Disease Models, Animal
OTO - NOTNLM
OT  - Airway inflammation
OT  - Alstonia scholaris (L.) R. Br.
OT  - Asthma
OT  - Th2 cytokines
OT  - Total alkaloids
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/07/18 01:09
MHDA- 2023/09/18 12:44
CRDT- 2023/07/17 19:22
PHST- 2023/04/24 00:00 [received]
PHST- 2023/07/05 00:00 [revised]
PHST- 2023/07/07 00:00 [accepted]
PHST- 2023/09/18 12:44 [medline]
PHST- 2023/07/18 01:09 [pubmed]
PHST- 2023/07/17 19:22 [entrez]
AID - S0378-8741(23)00755-9 [pii]
AID - 10.1016/j.jep.2023.116887 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2024 Jan 10;318(Pt A):116887. doi: 10.1016/j.jep.2023.116887. 
      Epub 2023 Jul 17.