PMID- 37463493
OWN - NLM
STAT- MEDLINE
DCOM- 20231218
LR  - 20231218
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 57
IP  - 46
DP  - 2023 Nov 21
TI  - Anammox Coupled with Photocatalyst for Enhanced Nitrogen Removal and the 
      Activated Aerobic Respiration of Anammox Bacteria Based on cbb3-Type Cytochrome c 
      Oxidase.
PG  - 17910-17919
LID - 10.1021/acs.est.3c02435 [doi]
AB  - This study introduced photogenerated electrons into the anammox system by 
      coupling them to a g-C(3)N(4) nanoparticle photocatalyst. A high nitrogen removal 
      efficiency (94.25%) was achieved, exceeding the biochemical limit of 89% imposed 
      by anammox stoichiometry. Photogenerated electrons boosted anammox metabolic 
      activity by empowering key enzymes (NIR, HZS, and WLP-related proteins) and 
      triggered rapid algal enrichment by enhancing the algal Calvin cycle, thus 
      developing multiple anammox-algae synergistic nitrogen removal processes. 
      Remarkably, the homologous expression of cbb3-type cytochrome c oxidase (CcO) in 
      anammox bacteria was discovered and reported in this study for the first time. 
      This conferred aerobic respiration capability to anammox bacteria and rendered 
      them the principal oxygen consumer under 7.9-19.8 mg/L dissolved oxygen, 
      originating from algal photosynthesis. Additionally, photogenerated electrons 
      selectively targeted the cb1 complex and cbb3-type CcO as activation sites while 
      mobilizing the RegA/B regulatory system to activate the expression of cbb3-type 
      CcO. Furthermore, cbb3-type CcO blocked oxidative stress in anammox by depleting 
      intracellular oxygen, a substrate for reactive oxygen species synthesis. This 
      optimized the environmental sensitivity of anammox bacteria and maintained their 
      high metabolic activity. This study expands our understanding of the 
      physiological aptitudes of anammox bacteria and provides valuable insights into 
      applying solar energy for enhanced wastewater treatment.
FAU - Zhang, Li
AU  - Zhang L
AD  - National Engineering Laboratory for Advanced Municipal Wastewater Treatment and 
      Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water 
      Environment Recovery Engineering, Beijing 100124, China.
FAU - Dong, Tingjun
AU  - Dong T
AUID- ORCID: 0009-0001-7905-0670
AD  - National Engineering Laboratory for Advanced Municipal Wastewater Treatment and 
      Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water 
      Environment Recovery Engineering, Beijing 100124, China.
FAU - Yang, Jiachun
AU  - Yang J
AD  - Shuifa Shandong Water Development Group Co. Ltd., Shandong 274200, China.
AD  - Shandong Jianzhu University, Shandong 250101, China.
FAU - Hao, Shiwei
AU  - Hao S
AUID- ORCID: 0000-0003-2495-8136
AD  - National Engineering Laboratory for Advanced Municipal Wastewater Treatment and 
      Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water 
      Environment Recovery Engineering, Beijing 100124, China.
FAU - Sun, Zaicheng
AU  - Sun Z
AUID- ORCID: 0000-0002-7059-3177
AD  - Center of Excellence for Environmental Safety and Biological Effects, Beijing Key 
      Laboratory for Green Catalysis and Separation, Faculty of Environment and Life, 
      Beijing University of Technology, 100 Pingleyuan, Beijing 100124, PR China.
FAU - Peng, Yongzhen
AU  - Peng Y
AUID- ORCID: 0000-0002-4702-3774
AD  - National Engineering Laboratory for Advanced Municipal Wastewater Treatment and 
      Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water 
      Environment Recovery Engineering, Beijing 100124, China.
LA  - eng
PT  - Journal Article
DEP - 20230718
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - N762921K75 (Nitrogen)
RN  - S88TT14065 (Oxygen)
RN  - 0 (Sewage)
SB  - IM
MH  - *Denitrification
MH  - *Electron Transport Complex IV/metabolism
MH  - Nitrogen/metabolism
MH  - Anaerobic Ammonia Oxidation
MH  - Oxidation-Reduction
MH  - Bacteria/metabolism
MH  - Oxygen
MH  - Respiration
MH  - Bioreactors/microbiology
MH  - Sewage/microbiology
OTO - NOTNLM
OT  - anammox
OT  - enhanced nitrogen removal
OT  - meta-omics analysis
OT  - photogenerated electrons
OT  - solar energy
EDAT- 2023/07/18 18:42
MHDA- 2023/12/18 06:42
CRDT- 2023/07/18 17:13
PHST- 2023/12/18 06:42 [medline]
PHST- 2023/07/18 18:42 [pubmed]
PHST- 2023/07/18 17:13 [entrez]
AID - 10.1021/acs.est.3c02435 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2023 Nov 21;57(46):17910-17919. doi: 
      10.1021/acs.est.3c02435. Epub 2023 Jul 18.