PMID- 37467934
OWN - NLM
STAT- MEDLINE
DCOM- 20230830
LR  - 20230901
IS  - 1873-1708 (Electronic)
IS  - 0890-6238 (Linking)
VI  - 120
DP  - 2023 Sep
TI  - Prenatal exposure to crude oil vapor reduces differentiation potential of rat 
      fetal mesenchymal stem cells by regulating ERK1/2 and PI3K signaling pathways: 
      Protective effect of quercetin.
PG  - 108440
LID - S0890-6238(23)00114-4 [pii]
LID - 10.1016/j.reprotox.2023.108440 [doi]
AB  - It has been indicated that crude oil vapor (COV) induces tissue damage by several 
      molecular mechanisms. Quercetin (QT) as an important component of food with 
      antioxidant properties has a protective role against cell toxicity caused by many 
      pollutants. However, data related to the adverse effects of crude oil vapor (COV) 
      on stem cell fate and differentiation and the role of quercetin (QT) in 
      protecting stem cells against the toxicity caused by these pollutants is very 
      limited. This study aimed to explore the protective effect of QT against the 
      adverse effects of COV on fetal mesenchymal stem cells (fMSCs) differentiation. 
      Twenty-four pregnant Wistar rats were categorized into 4 groups including the 
      control, COV, COV+QT, and QT. Rats were exposed to COV from gestational day (GD) 
      0-15 and received QT by gavage. The fMSCs were isolated from fetuses, and cell 
      proliferation, differentiation potential, expression of osteogenesis and 
      adipogenesis-related genes, and phosphorylation of PI3K and ERK1/2 signaling 
      proteins were evaluated. The results showed that COV reduced the proliferation 
      and differentiation of fMSCs through the activation of PI3K and ERK1/2 signaling 
      pathways. Also, COV significantly decreased the expression of osteonectin, ALP, 
      BMP-6, Runx-2, PPARgamma, and CREBBP genes in differentiated cells. QT treatment 
      increased the proliferation and differentiation of fMSCs in COV-exposed rats. In 
      conclusion, our findings suggest that prenatal exposure to COV impaired fMSCs 
      differentiation and QT reduced the adverse effects of COV by regulating ERK1/2 
      and PI3K signaling pathways.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Sadeghi, Abbas
AU  - Sadeghi A
AD  - Department of Basic Science, Division of Anatomy and Embryology, Faculty of 
      Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
FAU - Khazaeel, Kaveh
AU  - Khazaeel K
AD  - Department of Basic Science, Division of Anatomy and Embryology, Faculty of 
      Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran; Stem Cells 
      and Transgenic Technology Research Center (STTRC), Shahid Chamran University of 
      Ahvaz, Ahvaz, Iran. Electronic address: k.khazaeil@scu.ac.ir.
FAU - Tabandeh, Mohammad Reza
AU  - Tabandeh MR
AD  - Stem Cells and Transgenic Technology Research Center (STTRC), Shahid Chamran 
      University of Ahvaz, Ahvaz, Iran; Department of Basic Sciences, Division of 
      Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid 
      Chamran University of Ahvaz, Ahvaz, Iran.
FAU - Nejaddehbashi, Fereshteh
AU  - Nejaddehbashi F
AD  - Cellular and Molecular Research Center, Medical Basic Sciences Institute, Ahvaz 
      Jundishapur University of Medical Sciences, Ahvaz, Iran.
FAU - Givi, Masoumeh Ezzati
AU  - Givi ME
AD  - Department of Basic Sciences, Division of Pharmacology and Toxicology, Faculty of 
      Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230717
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Female
MH  - Pregnancy
MH  - Rats
MH  - Cell Differentiation
MH  - Fetus/metabolism
MH  - MAP Kinase Signaling System
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Osteogenesis
MH  - Phosphatidylinositol 3-Kinases
MH  - *Prenatal Exposure Delayed Effects/metabolism
MH  - Quercetin/pharmacology
MH  - Rats, Wistar
MH  - Signal Transduction
OTO - NOTNLM
OT  - Adipogenesis
OT  - Crude oil
OT  - Mesenchymal stem cells
OT  - Osteogenesis
OT  - Quercetin
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/07/20 01:06
MHDA- 2023/08/28 06:43
CRDT- 2023/07/19 19:25
PHST- 2023/05/18 00:00 [received]
PHST- 2023/07/12 00:00 [revised]
PHST- 2023/07/14 00:00 [accepted]
PHST- 2023/08/28 06:43 [medline]
PHST- 2023/07/20 01:06 [pubmed]
PHST- 2023/07/19 19:25 [entrez]
AID - S0890-6238(23)00114-4 [pii]
AID - 10.1016/j.reprotox.2023.108440 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2023 Sep;120:108440. doi: 10.1016/j.reprotox.2023.108440. Epub 
      2023 Jul 17.