PMID- 37471465 OWN - NLM STAT- MEDLINE DCOM- 20231229 LR - 20240226 IS - 2326-5205 (Electronic) IS - 2326-5191 (Linking) VI - 76 IP - 1 DP - 2024 Jan TI - Shared and Distinct Gut Microbiota in Spondyloarthritis, Acute Anterior Uveitis, and Crohn's Disease. PG - 48-58 LID - 10.1002/art.42658 [doi] AB - OBJECTIVE: Spondyloarthritis (SpA) is a group of immune-mediated diseases highly concomitant with nonmusculoskeletal inflammatory disorders, such as acute anterior uveitis (AAU) and Crohn's disease (CD). The gut microbiome represents a promising avenue to elucidate shared and distinct underlying pathophysiology. METHODS: We performed 16S ribosomal RNA sequencing on stool samples of 277 patients (72 CD, 103 AAU, and 102 SpA) included in the German Spondyloarthritis Inception Cohort and 62 back pain controls without any inflammatory disorder. Discriminatory statistical methods were used to disentangle microbial disease signals from one another and a wide range of potential confounders. Patients were naive to or had not received treatment with biological disease-modifying antirheumatic drugs (DMARDs) for >3 months before enrollment, providing a better approximation of a true baseline disease signal. RESULTS: We identified a shared, immune-mediated disease signal represented by low abundances of Lachnospiraceae taxa relative to controls, most notably Fusicatenibacter, which was most abundant in controls receiving nonsteroidal antiinflammatory drug monotherapy and implied to partially mediate higher serum C-reactive protein. Patients with SpA showed an enrichment of Collinsella, whereas human leukocyte antigen (HLA)-B27+ individuals displayed enriched Faecalibacterium. CD patients had higher abundances of a Ruminococcus taxon, and previous conventional/synthetic DMARD therapy was associated with increased Akkermansia. CONCLUSION: Our work supports the existence of a common gut dysbiosis in SpA and related inflammatory pathologies. We reveal shared and disease-specific microbial associations and suggest potential mediators of disease activity. Validation studies are needed to clarify the role of Fusicatenibacter in gut-joint inflammation, and metagenomic resolution is needed to understand the relationship between Faecalibacterium commensals and HLA-B27. CI - (c) 2023 The Authors. Arthritis & Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology. FAU - Essex, Morgan AU - Essex M AUID- ORCID: 0000-0001-8758-7497 AD - Experimental and Clinical Research Center (ECRC; a cooperation of the Max Delbruck Center and Charite-Universitatsmedizin), Max Delbruck Center for Molecular Medicine in the Helmholtz Association (MDC), and Charite-Universitatsmedizin Berlin (a corporate member of Freie Universitat Berlin and Humboldt-Universitat zu Berlin), Berlin, Germany. FAU - Rios Rodriguez, Valeria AU - Rios Rodriguez V AUID- ORCID: 0000-0001-5612-043X AD - Medical Department of Gastroenterology, Infectiology and Rheumatology, Campus Benjamin Franklin, Charite-Universitatsmedizin Berlin, Berlin, Germany. FAU - Rademacher, Judith AU - Rademacher J AUID- ORCID: 0000-0001-7442-2570 AD - Medical Department of Gastroenterology, Infectiology and Rheumatology, Campus Benjamin Franklin, Charite-Universitatsmedizin Berlin, and Berlin Institute of Health (BIH) at Charite-Universitatsmedizin Berlin, Berlin, Germany. FAU - Proft, Fabian AU - Proft F AUID- ORCID: 0000-0003-4306-033X AD - Medical Department of Gastroenterology, Infectiology and Rheumatology, Campus Benjamin Franklin, Charite-Universitatsmedizin Berlin, Berlin, Germany. FAU - Lober, Ulrike AU - Lober U AD - ECRC, MDC, Charite-Universitatsmedizin Berlin and German Center for Cardiovascular Research (DZHK), Berlin, Germany. FAU - Marko, Lajos AU - Marko L AD - ECRC, MDC, Charite-Universitatsmedizin Berlin and German Center for Cardiovascular Research (DZHK), Berlin, Germany. FAU - Pleyer, Uwe AU - Pleyer U AD - Department of Ophthalmology, Campus Virchow, Charite-Universitatsmedizin Berlin, Berlin, Germany. FAU - Strowig, Till AU - Strowig T AD - Helmholtz Center for Infection Research, Braunschweig, Germany, and Cluster of Excellence RESIST (EXC 2155), Hannover Medical School and Center for Individualized Infection Medicine (CiiM; a joint venture between the Helmholtz Center for Infection Research and the Hannover Medical School), Hannover, Germany. FAU - Marchand, Jeremy AU - Marchand J AD - MDC and BIH Metabolomics Platform at Charite-Universitatsmedizin Berlin, Berlin, Germany. FAU - Kirwan, Jennifer A AU - Kirwan JA AD - MDC and BIH Metabolomics Platform at Charite-Universitatsmedizin Berlin, Berlin, Germany, and University of Nottingham School of Veterinary Medicine and Science, Loughborough, UK. FAU - Siegmund, Britta AU - Siegmund B AD - Medical Department of Gastroenterology, Infectiology and Rheumatology, Campus Benjamin Franklin, Charite-Universitatsmedizin Berlin, Berlin, Germany. FAU - Forslund, Sofia K AU - Forslund SK AD - ECRC, MDC, Charite-Universitatsmedizin Berlin, and DZHK, Berlin, and Structural and Computational Biology Unit, EMBL, Heidelberg, Germany. FAU - Poddubnyy, Denis AU - Poddubnyy D AUID- ORCID: 0000-0002-4537-6015 AD - Department of Gastroentergology, Infectiology and Rheumatology, Campus Benjamin Franklin, Charite-Universitatsmedizin Berlin and German Rheumatism Research Center (DRFZ), Berlin, Germany. LA - eng GR - Food allergy and tolerance (FOOD@-Project ID 428046232)/Deutsche Forschungsgemeinschaft/ GR - CRC1449/Deutsche Forschungsgemeinschaft/ GR - CRC-TRR241/Deutsche Forschungsgemeinschaft/ GR - EXC 2155 "RESIST" (Project ID 390874280/Deutsche Forschungsgemeinschaft/ GR - German Research Foundation/ GR - German Federal Ministry for Health and Research (BMBF)/ GR - Novartis/ GR - BIH-Charite Clinician Scientist Program/ GR - Charite-Universitatsmedizin Berlin/ PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20231019 PL - United States TA - Arthritis Rheumatol JT - Arthritis & rheumatology (Hoboken, N.J.) JID - 101623795 RN - 0 (Antirheumatic Agents) RN - 0 (HLA-B27 Antigen) SB - IM MH - Humans MH - *Crohn Disease/drug therapy/complications MH - *Gastrointestinal Microbiome/genetics MH - *Spondylarthritis/drug therapy/complications MH - *Uveitis, Anterior/drug therapy MH - *Antirheumatic Agents MH - Clostridiales/metabolism MH - HLA-B27 Antigen/genetics MH - Acute Disease EDAT- 2023/07/20 19:14 MHDA- 2023/12/29 06:43 CRDT- 2023/07/20 13:54 PHST- 2023/06/26 00:00 [revised] PHST- 2022/09/18 00:00 [received] PHST- 2023/07/13 00:00 [accepted] PHST- 2023/12/29 06:43 [medline] PHST- 2023/07/20 19:14 [pubmed] PHST- 2023/07/20 13:54 [entrez] AID - 10.1002/art.42658 [doi] PST - ppublish SO - Arthritis Rheumatol. 2024 Jan;76(1):48-58. doi: 10.1002/art.42658. Epub 2023 Oct 19.