PMID- 37474982
OWN - NLM
STAT- MEDLINE
DCOM- 20230724
LR  - 20230724
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 13
DP  - 2023 Jul
TI  - [Analysis and evaluation of bioactive constituents from different parts of 
      Epimedium brevicornum].
PG  - 3448-3461
LID - 10.19540/j.cnki.cjcmm.20230313.102 [doi]
AB  - A comprehensive analytical method based on ultra-fast liquid chromatography 
      coupled with triple quadrupole/linear ion trap tandem mass 
      spectrometry(UFLC-QTRAP-MS/MS) was established for simultaneous determination of 
      the content of 45 bioactive constituents including flavonoids, alkaloids, amino 
      acids, phenolic acids, and nucleosides in Epimedium brevicornum. The multiple 
      bioactive constituents in leaves, petioles, stems and rhizomes of E. brevicornum 
      were analyzed. The gradient elution was performed at 30 ℃ in an XBridge~(R) C_(18) 
      column(4.6 mmx100 mm, 3.5 mum) with 0.4% formic acid aqueous solution-acetonitrile 
      as the mobile phase at a flow rate of 0.8 mL.min~(-1). Single factor experiment 
      and response surface methodology were employed to optimize the extraction 
      conditions. Multivariate statistical analyses including systematic cluster 
      analysis(SCA), principal component analysis(PCA), partial least squares 
      discriminant analysis(PLS-DA), and one-way analysis of variance(One-way ANOVA) 
      were carried out to classify the samples from different parts and identify 
      different constituents. Grey relation analysis(GRA) and entropy weight-TOPSIS 
      analysis were performed to build a multi-index comprehensive evaluation model for 
      different parts of E. brevicornum. The results showed that there was a good 
      relationship between the mass concentrations of 45 constituents and the 
      corresponding peak areas, with the correlation coefficients(r) not less than 
      0.999 0. The precision, repeatability, and stability of the established method 
      were good for all the target constituents in this study, with the relative 
      standard deviations(RSDs) less than 5.0%(0.62%-4.9%) and the average recovery of 
      94.51%-105.7%. The above results indicated that the bioactive constituents varied 
      in different parts of E. brevicornum, and the overall quality followed the trend 
      of leaves > petioles > rhizomes > stems. This study verified the 
      rationality of the Chinese Pharmacopoeia(2020 edition) stipulating that the 
      medicinal part of E. brevicornum is the leaf. Moreover, our study indicated that 
      the rhizome had the potential for medicinal development. The established method 
      was accurate and reliable, which can be used to comprehensive evaluate and 
      control the quality of E. brevicornum. This study provides data reference for 
      clarifying the medicinal parts and rationally utilizing the resources of E. 
      brevicornum.
FAU - Xue, Jia
AU  - Xue J
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Chen, Hai-Jie
AU  - Chen HJ
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Zhou, Yong-Yi
AU  - Zhou YY
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Yuan, Jia-Huan
AU  - Yuan JH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Cai, Zhi-Chen
AU  - Cai ZC
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Wu, Nan
AU  - Wu N
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Chen, Cui-Hua
AU  - Chen CH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Liu, Xun-Hong
AU  - Liu XH
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China 
      Jiangsu Province Engineering Research Center of Classical Prescription Nanjing 
      210023, China.
FAU - Zou, Li-Si
AU  - Zou LS
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Yin, Sheng-Xin
AU  - Yin SX
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Yang, Wei
AU  - Yang W
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
FAU - Cheng, Jian-Ming
AU  - Cheng JM
AD  - School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China 
      Jiangsu Province Engineering Research Center of Classical Prescription Nanjing 
      210023, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - *Epimedium
MH  - Tandem Mass Spectrometry
MH  - Chromatography, Liquid
MH  - Multivariate Analysis
OTO - NOTNLM
OT  - Epimedium brevicornum
OT  - UFLC-QTRAP-MS/MS
OT  - different parts
OT  - multiple bioactive constituents
OT  - multivariate statistical analysis
EDAT- 2023/07/21 01:11
MHDA- 2023/07/24 06:42
CRDT- 2023/07/20 23:44
PHST- 2023/07/24 06:42 [medline]
PHST- 2023/07/21 01:11 [pubmed]
PHST- 2023/07/20 23:44 [entrez]
AID - 10.19540/j.cnki.cjcmm.20230313.102 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Jul;48(13):3448-3461. doi: 
      10.19540/j.cnki.cjcmm.20230313.102.