PMID- 37478352
OWN - NLM
STAT- Publisher
LR  - 20230731
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 57
IP  - 30
DP  - 2023 Aug 1
TI  - Marine Dehalogenator and Its Chaperones: Microbial Duties and Responses in 
      2,4,6-Trichlorophenol Dechlorination.
PG  - 11300-11312
LID - 10.1021/acs.est.3c03738 [doi]
AB  - Marine environments contain diverse halogenated organic compounds (HOCs), both 
      anthropogenic and natural, nourishing a group of versatile organohalide-respiring 
      bacteria (OHRB). Here, we identified a novel OHRB (Peptococcaceae DCH) with 
      conserved motifs but phylogenetically diverse reductive dehalogenase catalytic 
      subunit (RdhAs) from marine enrichment culture. Further analyses clearly 
      demonstrate the horizontal gene transfer of rdhAs among marine OHRB. Moreover, 
      2,4,6-trichlorophenol (TCP) was dechlorinated to 2,4-dichlorophenol and 
      terminated at 4-chlorophenol in culture. Dendrosporobacter and Methanosarcina 
      were the two dominant genera, and the constructed and verified metabolic pathways 
      clearly demonstrated that the former provided various substrates for other 
      microbes, while the latter drew nutrients, but might provide little benefit to 
      microbial dehalogenation. Furthermore, Dendrosporobacter could readily adapt to 
      TCP, and sporulation-related proteins of Dendrosporobacter were significantly 
      upregulated in TCP-free controls, whereas other microbes (e.g., Methanosarcina 
      and Aminivibrio) became more active, providing insights into how HOCs shape 
      microbial communities. Additionally, sulfate could affect the dechlorination of 
      Peptococcaceae DCH, but not debromination. Considering their electron 
      accessibility and energy generation, the results clearly demonstrate that 
      bromophenols are more suitable than chlorophenols for the enrichment of OHRB in 
      marine environments. This study will greatly enhance our understanding of marine 
      OHRB (rdhAs), auxiliary microbes, and microbial HOC adaptive mechanisms.
FAU - Deng, Zhaochao
AU  - Deng Z
AUID- ORCID: 0000-0001-7732-276X
AD  - Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, 
      Zhoushan 316021, Zhejiang, China.
FAU - Chen, Haixin
AU  - Chen H
AD  - BGI-Sanya, BGI-Shenzhen, Sanya 572025, China.
FAU - Wang, Jun
AU  - Wang J
AD  - BGI-Sanya, BGI-Shenzhen, Sanya 572025, China.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Department of Environmental Engineering, School of Chemical Engineering and 
      Pharmacy, Henan University of Science and Technology, Luoyang 471000, Henan, 
      China.
FAU - Han, Zhiqiang
AU  - Han Z
AD  - Department of Marine Resources and Environment, Fishery College, Zhejiang Ocean 
      University, Zhoushan 316002, Zhejiang, China.
FAU - Xie, Yeting
AU  - Xie Y
AD  - College of Environmental Science and Engineering, Guilin University of 
      Technology, Guilin 541006, Guangxi, China.
FAU - Zhang, Xiaoyan
AU  - Zhang X
AD  - College of Environmental Science and Engineering, Guilin University of 
      Technology, Guilin 541006, Guangxi, China.
FAU - Fang, Xiaodong
AU  - Fang X
AD  - BGI-Sanya, BGI-Shenzhen, Sanya 572025, China.
FAU - Yu, Hao
AU  - Yu H
AD  - Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, 
      Zhoushan 316021, Zhejiang, China.
FAU - Zhang, Dongdong
AU  - Zhang D
AD  - Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, 
      Zhoushan 316021, Zhejiang, China.
FAU - Yue, Zhen
AU  - Yue Z
AD  - BGI-Sanya, BGI-Shenzhen, Sanya 572025, China.
FAU - Zhang, Chunfang
AU  - Zhang C
AD  - Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, 
      Zhoushan 316021, Zhejiang, China.
AD  - College of Environmental Science and Engineering, Guilin University of 
      Technology, Guilin 541006, Guangxi, China.
LA  - eng
PT  - Journal Article
DEP - 20230721
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
SB  - IM
OTO - NOTNLM
OT  - Dendrosporobacter
OT  - Peptococcaceae
OT  - methanogens
OT  - microbial responses
OT  - multiomics
OT  - rdhA genes
EDAT- 2023/07/21 19:10
MHDA- 2023/07/21 19:10
CRDT- 2023/07/21 15:23
PHST- 2023/07/21 19:10 [pubmed]
PHST- 2023/07/21 19:10 [medline]
PHST- 2023/07/21 15:23 [entrez]
AID - 10.1021/acs.est.3c03738 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2023 Aug 1;57(30):11300-11312. doi: 10.1021/acs.est.3c03738. 
      Epub 2023 Jul 21.