PMID- 37480371
OWN - NLM
STAT- MEDLINE
DCOM- 20230821
LR  - 20230821
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 107
IP  - 18
DP  - 2023 Sep
TI  - Steerable artificial magnetic bacteria with target delivery ability of calcium 
      carbonate for soil improvement.
PG  - 5687-5700
LID - 10.1007/s00253-023-12665-3 [doi]
AB  - The microbial-induced carbonate precipitation (MICP) has acquired significant 
      attention due to its immense potential in sustainable engineering applications, 
      particularly in soil improvement. However, the precise control of 
      microbial-induced calcium carbonate precipitation remains a formidable challenge 
      in engineering practices, owing to the uncertain movement paths of bacteria and 
      the nonuniform distribution of soil pores. Taking inspiration from targeted 
      therapy in medicine, this paper presents novel research on the development and 
      validation of magnetically responsive bacteria. These bacteria demonstrate the 
      ability to target calcium carbonate precipitation in a microfluidic chip, thereby 
      promoting an environmentally friendly and ecologically sustainable 
      biomineralization paradigm. The study focuses on investigating the migration of 
      magnetite nanoparticles (MNPs) in aqueous solutions and enhancing the stability 
      of MNP culture liquids. A specially designed microfluidic chip is utilized to 
      simulate natural sand particles and their pores, while an external magnetic field 
      is applied to precisely control the movement path of the artificial magnetic 
      bacteria, enabling targeted precipitation of calcium carbonate at the 
      micron-scale. Verification of the engineered artificial magnetic bacteria and 
      their ability to induce calcium carbonate precipitation is conducted through 
      SEM-EDS analysis, microfluidic chip observations, and the application of the 
      K-means algorithm and ImageJ software to analyze calcium carbonate formation. The 
      influence of the concentration of magnetic nanoparticles on the calcium carbonate 
      production rate was also studied. The results confirm the potential of the 
      artificial magnetic bacteria for future engineering applications. KEY POINTS: * 
      Sporosarcina pasteurii is first time successfully engineered into artificial 
      magnetic bacteria. * The artificial magnetic bacteria show excellent performance 
      of targeted transportation and directional deposition of CaCO(3) in microfluidic 
      chip. * The emergence of artificial magnetic bacteria promotes paradigm shift of 
      next generation environmentally friendly biomineralization.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Wang, Shiqing
AU  - Wang S
AD  - Key Laboratory of Building Safety and Energy Efficiency of the Ministry of 
      Education, Hunan University, Changsha, 410082, China.
AD  - Research Center for Advanced Underground, Space Technologies of Hunan University, 
      Changsha, 410082, China.
AD  - College of Civil Engineering, Hunan University, Changsha, 410082, China.
FAU - Chen, Yongqing
AU  - Chen Y
AD  - Key Laboratory of Building Safety and Energy Efficiency of the Ministry of 
      Education, Hunan University, Changsha, 410082, China.
AD  - Research Center for Advanced Underground, Space Technologies of Hunan University, 
      Changsha, 410082, China.
AD  - College of Civil Engineering, Hunan University, Changsha, 410082, China.
AD  - A School of Transportation Engineering, East China Jiaotong University, Nanchang 
      Jiangxi 330013, China.
FAU - Chen, Renpeng
AU  - Chen R
AD  - Key Laboratory of Building Safety and Energy Efficiency of the Ministry of 
      Education, Hunan University, Changsha, 410082, China.
AD  - Research Center for Advanced Underground, Space Technologies of Hunan University, 
      Changsha, 410082, China.
AD  - College of Civil Engineering, Hunan University, Changsha, 410082, China.
FAU - Ma, Xiongying
AU  - Ma X
AD  - Key Laboratory of Building Safety and Energy Efficiency of the Ministry of 
      Education, Hunan University, Changsha, 410082, China.
AD  - Research Center for Advanced Underground, Space Technologies of Hunan University, 
      Changsha, 410082, China.
AD  - College of Civil Engineering, Hunan University, Changsha, 410082, China.
FAU - Kang, Xin
AU  - Kang X
AUID- ORCID: 0000-0002-9758-9100
AD  - Key Laboratory of Building Safety and Energy Efficiency of the Ministry of 
      Education, Hunan University, Changsha, 410082, China. kangxin@hnu.edu.cn.
AD  - Research Center for Advanced Underground, Space Technologies of Hunan University, 
      Changsha, 410082, China. kangxin@hnu.edu.cn.
AD  - College of Civil Engineering, Hunan University, Changsha, 410082, China. 
      kangxin@hnu.edu.cn.
LA  - eng
GR  - No. 52090082/National Natural Science Foundation of China/
GR  - 2019RS1030/Science and Technology Innovative Research Team in Higher Educational 
      Institutions of Hunan Province/
PT  - Journal Article
DEP - 20230722
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 0 (Soil)
SB  - IM
MH  - *Calcium Carbonate
MH  - *Soil
MH  - Algorithms
MH  - Bacteria
MH  - Magnetic Fields
OTO - NOTNLM
OT  - Calcium carbonate precipitation
OT  - Magnetite nanoparticles
OT  - Microfluidic chip
OT  - Sporosarcina pasteurii
EDAT- 2023/07/22 21:06
MHDA- 2023/08/21 06:42
CRDT- 2023/07/22 11:03
PHST- 2022/11/28 00:00 [received]
PHST- 2023/06/27 00:00 [accepted]
PHST- 2023/06/22 00:00 [revised]
PHST- 2023/08/21 06:42 [medline]
PHST- 2023/07/22 21:06 [pubmed]
PHST- 2023/07/22 11:03 [entrez]
AID - 10.1007/s00253-023-12665-3 [pii]
AID - 10.1007/s00253-023-12665-3 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2023 Sep;107(18):5687-5700. doi: 
      10.1007/s00253-023-12665-3. Epub 2023 Jul 22.