PMID- 37480618
OWN - NLM
STAT- Publisher
LR  - 20230812
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 154
DP  - 2023 Oct
TI  - Long non-coding RNA SLC7A11 antisense RNA1 promotes oral squamous cell carcinoma 
      progression by regulating ubiquitination of K-homology type splicing regulatory 
      protein.
PG  - 105762
LID - S0003-9969(23)00150-4 [pii]
LID - 10.1016/j.archoralbio.2023.105762 [doi]
AB  - OBJECTIVES: This article aims to elucidate the role of Long non-coding RNA 
      SLC7A11 antisense RNA1 (SLC7A11-AS1) in oral squamous cell carcinoma, which are 
      expected to be useful for the oral squamous cell carcinoma diagnosis and 
      treatment. DESIGN: SLC7A11-AS1 expression was detected by quantitative real-time 
      reverse transcription polymerase chain reaction (qRT-PCR) in oral squamous cell 
      carcinoma cell lines. Cellular localization of SLC7A11-AS1C was detected by 
      fluorescence in situ hybridization (FISH) assays and subcellular fractionation 
      assay. Biological functions of SLC7A11-AS1 were explored by 
      3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyl-2-H-tetrazolium bromide (MTT), 
      5-ethynyl-2'-deoxyuridine (EdU), wounding healing, and transwell invasion assays 
      in vitro, as well as mice xenograft experiments and metastasis assays in vivo. 
      RNA pull-down, RNA immunoprecipitation, co-immunoprecipitation, ubiquitination 
      assays, and rescue experiments were performed to determine the molecular 
      mechanism of SLC7A11-AS1 in oral squamous cell carcinoma. RESULTS: SLC7A11-AS1 is 
      overexpressed in oral cancer tissues and cell lines. Functionally, knockdown of 
      SLC7A11-AS1 reduced the proliferation, migration, and invasion of oral squamous 
      cell carcinoma cells in vitro and inhibited tumor growth as well as metastasis in 
      vivo. Mechanistically, SLC7A11-AS1 impeded the interaction between K-homology 
      type splicing regulatory protein (KHSRP) and kelch-like 12 (KLHL12), maintaining 
      the stability of KHSRP by restraining KHSRP degradation through the 
      ubiquitination-proteasome pathway. Furthermore, KHSRP overexpression recovered 
      the malignant behaviors inhibited by SLC7A11-AS1 knockdown in oral cancer cells. 
      CONCLUSION: SLC7A11-AS1 promoted oral squamous cell carcinoma development by 
      interacting with KHSRP and maintaining KHSRP stability by preventing its 
      degradation via the ubiquitination-proteasome pathway. Thus, SLC7A11-AS1 is a 
      potential therapeutic target for oral cancer.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Liu, Zhen
AU  - Liu Z
AD  - Department of Stomatology, the Eighth Medical Center of Chinese PLA General 
      Hospital, No.17 heishanhu Road, Haidian District, Beijing 100091, China.
FAU - Wang, Xiaoyu
AU  - Wang X
AD  - Department of Stomatology, PLA Strategic Support Force Medical Center, No.9 
      Anxiang Beili, Deshengmenwai, Chaoyang District, Beijing 100101, China.
FAU - Liu, Lin
AU  - Liu L
AD  - Department of Stomatology, the First Medical Center of Chinese PLA General 
      Hospital, No.28 Fuxing Road, Haidian District, Beijing 100853, China.
FAU - Guan, Miaosheng
AU  - Guan M
AD  - Department of Stomatology, PLA Rocket Force Characteristic Medical Center, No.16 
      Xinjiekou outside Street, Haidian District, Beijing 100088, China.
FAU - Jiang, Hao
AU  - Jiang H
AD  - Department of Stomatology, the Eighth Medical Center of Chinese PLA General 
      Hospital, No.17 heishanhu Road, Haidian District, Beijing 100091, China.
FAU - An, Dan
AU  - An D
AD  - Department of Stomatology, the Eighth Medical Center of Chinese PLA General 
      Hospital, No.17 heishanhu Road, Haidian District, Beijing 100091, China.
FAU - Li, Hongbo
AU  - Li H
AD  - Department of Stomatology, the First Medical Center of Chinese PLA General 
      Hospital, No.28 Fuxing Road, Haidian District, Beijing 100853, China. Electronic 
      address: hongbo_l@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20230711
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
SB  - IM
OTO - NOTNLM
OT  - Invasion
OT  - KH-type splicing regulatory protein
OT  - Migration
OT  - Oral squamous cell carcinoma
OT  - Proliferation
OT  - SLC7A11 antisense RNA1
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/07/23 01:11
MHDA- 2023/07/23 01:11
CRDT- 2023/07/22 18:01
PHST- 2023/04/12 00:00 [received]
PHST- 2023/06/21 00:00 [revised]
PHST- 2023/07/03 00:00 [accepted]
PHST- 2023/07/23 01:11 [pubmed]
PHST- 2023/07/23 01:11 [medline]
PHST- 2023/07/22 18:01 [entrez]
AID - S0003-9969(23)00150-4 [pii]
AID - 10.1016/j.archoralbio.2023.105762 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2023 Oct;154:105762. doi: 10.1016/j.archoralbio.2023.105762. Epub 
      2023 Jul 11.