PMID- 37481983 OWN - NLM STAT- MEDLINE DCOM- 20230824 LR - 20230824 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 429 DP - 2023 Dec 15 TI - Small molecule linoleic acid inhibiting whey syneresis via interact with milk proteins in the fermentation of set yogurt fortified with c9,t11-conjugated linoleic acid. PG - 136849 LID - S0308-8146(23)01467-X [pii] LID - 10.1016/j.foodchem.2023.136849 [doi] AB - The study aimed to investigate the impact of fermentation conditions on c9,t11-conjugated linoleic acid (CLA) synthesis by Lactobacillus casei, as well as its effects on whey syneresis, water holding capacity (WHC), and texture characteristics of set yogurt. The amount of whey syneresis decreased about 30% with the adding of 0.1% linoleic acid (LA). The interaction between LA and casein (CS), beta-lactoglobulin (beta-Lg) and bovine serum albumin (BSA) was observed by UV-Vis absorption spectroscopy, 3D fluorescence spectroscopy and CD spectroscopy. It found that LA changed the microenvironment and polarity around amino acids, as well as the conformation of the three milk proteins. Scanning electron microscope (SEM) analysis revealed that the addition of LA resulted in a more uniform and compact microstructure of the set yogurt. It indicates that LA can promote the crosslink of milk proteins, which may be the reason for the reduction of whey syneresis in set yogurt. CI - Copyright (c) 2023 Elsevier Ltd. All rights reserved. FAU - Qing, Junjun AU - Qing J AD - Sino-German Joint Research Institute, Nanchang University, Nanchang, China. FAU - Peng, Cheng AU - Peng C AD - Sino-German Joint Research Institute, Nanchang University, Nanchang, China. FAU - Chen, Hongbing AU - Chen H AD - Sino-German Joint Research Institute, Nanchang University, Nanchang, China; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, China; International Institute of Food Innovation, Nanchang University, Nanchang, China. FAU - Li, Haixing AU - Li H AD - Sino-German Joint Research Institute, Nanchang University, Nanchang, China; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, China; International Institute of Food Innovation, Nanchang University, Nanchang, China. FAU - Liu, Xiaohua AU - Liu X AD - Sino-German Joint Research Institute, Nanchang University, Nanchang, China; International Institute of Food Innovation, Nanchang University, Nanchang, China. Electronic address: liuxiaohua@ncu.edu.cn. LA - eng PT - Journal Article DEP - 20230710 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 0 (Milk Proteins) RN - 9KJL21T0QJ (Linoleic Acid) RN - 0 (Linoleic Acids, Conjugated) RN - 0 (Whey Proteins) SB - IM MH - *Milk Proteins/chemistry MH - Whey/metabolism MH - Linoleic Acid/pharmacology MH - *Linoleic Acids, Conjugated/metabolism MH - Yogurt/analysis MH - Fermentation MH - Whey Proteins/chemistry OTO - NOTNLM OT - Linoleic acid OT - Set yogurt OT - Whey syneresis OT - c9, t11-Conjugated linoleic acid COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/07/24 00:41 MHDA- 2023/08/24 06:42 CRDT- 2023/07/23 18:08 PHST- 2023/04/04 00:00 [received] PHST- 2023/06/21 00:00 [revised] PHST- 2023/07/08 00:00 [accepted] PHST- 2023/08/24 06:42 [medline] PHST- 2023/07/24 00:41 [pubmed] PHST- 2023/07/23 18:08 [entrez] AID - S0308-8146(23)01467-X [pii] AID - 10.1016/j.foodchem.2023.136849 [doi] PST - ppublish SO - Food Chem. 2023 Dec 15;429:136849. doi: 10.1016/j.foodchem.2023.136849. Epub 2023 Jul 10.