PMID- 37483283 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230725 IS - 2297-1769 (Print) IS - 2297-1769 (Electronic) IS - 2297-1769 (Linking) VI - 10 DP - 2023 TI - Isolation, identification, and pathogenicity of a NADC30-like porcine reproductive and respiratory disorder syndrome virus strain affecting sow production. PG - 1207189 LID - 10.3389/fvets.2023.1207189 [doi] LID - 1207189 AB - Since it was first reported in 1987, porcine reproductive and respiratory syndrome virus (PRRSV) has caused several economic crises worldwide. The current prevalence of PRRSV NADC30-like stains causing clinical disease outbreaks in Chain is highly concerning. Immunization against and the prevention of this infection are burdensome for farming organizations as the pathogen frequently mutates and undergoes recombination. Herein, the genetic characterization of a NADC30-like strain (termed BL2019) isolated from a farm in Guangdong Province, China, was analyzed and its pathogenicity for piglets and sows was assessed. Results revealed that BL2019 exhibits a nucleotide homology of 93.7% with NADC30 PRRSV and its NSP2 coding region demonstrates the same 131aa deletion pattern as that of NADC30 and NADC30-like. Furthermore, we identified two recombination breakpoints located nt5804 of the NSP5-coding region and nt6478 of NSP2-coding region, the gene fragment between the two breakpoints showed higher homology to the TJ strain(a representative strain of highly pathogenic PRRSV) compared to the NADC30 strain. In addition, BL2019 infection in piglets caused fever lasting for 1 week, moderate respiratory clinical signs and obvious visual and microscopic lung lesions; infection in gestating sows affected their feed intake and increased body temperature, abortion rates, number of weak fetuses, and other undesirable phenomena. Therefore, we report a NADC30-like PRRSV strain with partial recombination and a representative strain of HP-PRRSV, strain TJ, that can provide early warning and support for PRRS immune prevention and control. CI - Copyright (c) 2023 Chang, Zheng, Qiu, Chen, Li, Wu, Lin, Zhao, Zhou, Gong, Sun, Zhang and Wang. FAU - Chang, Hao AU - Chang H AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. AD - Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, China. AD - National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China. FAU - Zheng, Jiaying AU - Zheng J AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. FAU - Qiu, Yingwu AU - Qiu Y AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. AD - Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China. FAU - Chen, Chuanxin AU - Chen C AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. FAU - Li, Qunhui AU - Li Q AD - Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China. FAU - Wu, Qianwen AU - Wu Q AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. FAU - Lin, Limiao AU - Lin L AD - Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China. FAU - Zhao, Haishen AU - Zhao H AD - Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China. FAU - Zhou, Qingfeng AU - Zhou Q AD - Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China. FAU - Gong, Lang AU - Gong L AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. AD - Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, China. FAU - Sun, Yankuo AU - Sun Y AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. AD - Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, China. AD - National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China. FAU - Zhang, Xiangbin AU - Zhang X AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. AD - Wen's Group Academy, Wen's Foodstuffs Group Co., Ltd., Xinxing, Guangdong, China. FAU - Wang, Heng AU - Wang H AD - Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. AD - Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, China. AD - National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China. LA - eng PT - Journal Article DEP - 20230707 PL - Switzerland TA - Front Vet Sci JT - Frontiers in veterinary science JID - 101666658 PMC - PMC10360194 OTO - NOTNLM OT - PRRSV OT - pathogenicity OT - phylogenetic analyses OT - recombination analyses OT - virus isolation and identification COIS- YQ, QL, LL, HZ, and QZ were employed by Wen's Foodstuffs Group Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2023/07/24 06:42 MHDA- 2023/07/24 06:43 PMCR- 2023/01/01 CRDT- 2023/07/24 04:27 PHST- 2023/04/17 00:00 [received] PHST- 2023/06/22 00:00 [accepted] PHST- 2023/07/24 06:43 [medline] PHST- 2023/07/24 06:42 [pubmed] PHST- 2023/07/24 04:27 [entrez] PHST- 2023/01/01 00:00 [pmc-release] AID - 10.3389/fvets.2023.1207189 [doi] PST - epublish SO - Front Vet Sci. 2023 Jul 7;10:1207189. doi: 10.3389/fvets.2023.1207189. eCollection 2023.