PMID- 37485537
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230725
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 14
DP  - 2023
TI  - Circadian regulation of metabolic, cell division, and cation transport promoters 
      in the gastrointestinal bacterium Klebsiella aerogenes.
PG  - 1181756
LID - 10.3389/fmicb.2023.1181756 [doi]
LID - 1181756
AB  - INTRODUCTION: All eukaryotes and at least some prokaryotes express the capacity 
      to anticipate and adapt to daily changes of light and temperature in their 
      environments. These circadian programs are fundamental features of many forms of 
      life. Cyanobacteria were the first prokaryotes to have demonstrated circadian 
      gene expression. Recently, a circadian rhythm was also discovered in an unrelated 
      bacterium, Klebsiella aerogenes, a human gut commensal and nosocomial pathogen. 
      METHODS: Here we characterize new clock-controlled genes with spatial differences 
      in expression using a bacterial luciferase reporter. These include 
      dephospho-coenzyme A kinase (coaE), manganese transporter, H-dependent (mntH) and 
      a gene identified as filamenting temperature-sensitive mutant Z (ftsZ). RESULTS 
      AND DISCUSSION: The data show that all three reporter constructs exhibited 
      circadian variation, although only PmntH::luxCDABE reporter strains were 
      synchronized by melatonin. Additionally, we show that K. aerogenes divides 
      rhythmically in vitro and that these bacteria may alternate between exponential 
      and stationary cells. Together, these findings provide a deeper understanding of 
      K. aerogenes.
CI  - Copyright (c) 2023 Graniczkowska, Paulose and Cassone.
FAU - Graniczkowska, Kinga B
AU  - Graniczkowska KB
AD  - Department of Biology, University of Kentucky, Lexington, KY, United States.
FAU - Paulose, Jiffin K
AU  - Paulose JK
AD  - Division of Human Genetics, Cincinnati Children's Hospital, Cincinnati, OH, 
      United States.
FAU - Cassone, Vincent M
AU  - Cassone VM
AD  - Department of Biology, University of Kentucky, Lexington, KY, United States.
LA  - eng
PT  - Journal Article
DEP - 20230705
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC10356819
OTO - NOTNLM
OT  - Klebsiella
OT  - circadian
OT  - gastrointestinal
OT  - melatonin
OT  - microbiome
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/07/24 06:42
MHDA- 2023/07/24 06:43
PMCR- 2023/07/05
CRDT- 2023/07/24 05:00
PHST- 2023/03/07 00:00 [received]
PHST- 2023/05/29 00:00 [accepted]
PHST- 2023/07/24 06:43 [medline]
PHST- 2023/07/24 06:42 [pubmed]
PHST- 2023/07/24 05:00 [entrez]
PHST- 2023/07/05 00:00 [pmc-release]
AID - 10.3389/fmicb.2023.1181756 [doi]
PST - epublish
SO  - Front Microbiol. 2023 Jul 5;14:1181756. doi: 10.3389/fmicb.2023.1181756. 
      eCollection 2023.