PMID- 37486783
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1521-4095 (Electronic)
IS  - 0935-9648 (Linking)
VI  - 35
IP  - 46
DP  - 2023 Nov
TI  - Multi-Body Biomarker Entrapment System: An All-Encompassing Tool for 
      Ultrasensitive Disease Diagnosis and Epidemic Screening.
PG  - e2304119
LID - 10.1002/adma.202304119 [doi]
AB  - Ultrasensitive identification of biomarkers in biofluids is essential for the 
      precise diagnosis of diseases. For the gold standard approaches, polymerase chain 
      reaction and enzyme-linked immunosorbent assay, cumbersome operational steps 
      hinder their point-of-care applications. Here, a bionic biomarker entrapment 
      system (BioES) is implemented, which employs a multi-body Y-shaped tetrahedral 
      DNA probe immobilized on carbon nanotube transistors. Clinical identification of 
      endometriosis is successfully realized by detecting an estrogen receptor, ERbeta, 
      from the lesion tissue of endometriosis patients and establishing a standard 
      diagnosis procedure. The multi-body Y-shaped BioES achieves a theoretical limit 
      of detection (LoD) of 6.74 aM and a limit of quantification of 141 aM in a 
      complex protein milieu. Furthermore, the BioES is optimized into a multi-site 
      recognition module for enhanced binding efficiency, realizing the first 
      identification of monkeypox virus antigen A35R and unamplified detection of 
      circulating tumor DNA of breast cancer in serum. The rigid and compact probe 
      framework with synergy effect enables the BioES to target A35R and DNA with a LoD 
      down to 991 and 0.21 aM, respectively. Owing to its versatility for proteins and 
      nucleic acids as well as ease of manipulation and ultra-sensitivity, the BioES 
      can be leveraged as an all-encompassing tool for population-wide screening of 
      epidemics and clinical disease diagnosis.
CI  - (c) 2023 Wiley-VCH GmbH.
FAU - Ren, Qinqi
AU  - Ren Q
AUID- ORCID: 0000-0002-1259-6353
AD  - School of Electronic and Computer Engineering, Peking University, Shenzhen, 
      518055, China.
FAU - Jiang, Leying
AU  - Jiang L
AD  - State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and 
      Biotechnology, Peking University, Shenzhen, 518055, China.
FAU - Ma, Shenhui
AU  - Ma S
AD  - School of Electronic and Computer Engineering, Peking University, Shenzhen, 
      518055, China.
FAU - Li, Tong
AU  - Li T
AD  - Department of Gynecology, Shenzhen People's Hospital (The Second Clinical Medical 
      College, Jinan University, The First Affiliated Hospital, Southern University of 
      Science and Technology), Shenzhen, Guangdong, 518020, China.
FAU - Zhu, Yang
AU  - Zhu Y
AD  - School of Electronic and Computer Engineering, Peking University, Shenzhen, 
      518055, China.
FAU - Qiu, Rui
AU  - Qiu R
AD  - School of Electronic and Computer Engineering, Peking University, Shenzhen, 
      518055, China.
FAU - Xing, Yun
AU  - Xing Y
AD  - State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and 
      Biotechnology, Peking University, Shenzhen, 518055, China.
FAU - Yin, Feng
AU  - Yin F
AD  - Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, Shenzhen, 
      518055, China.
FAU - Li, Zigang
AU  - Li Z
AD  - State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and 
      Biotechnology, Peking University, Shenzhen, 518055, China.
AD  - Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, Shenzhen, 
      518055, China.
FAU - Ye, Xiyang
AU  - Ye X
AD  - Department of Gynecology, Shenzhen People's Hospital (The Second Clinical Medical 
      College, Jinan University, The First Affiliated Hospital, Southern University of 
      Science and Technology), Shenzhen, Guangdong, 518020, China.
FAU - Zhang, Yaping
AU  - Zhang Y
AD  - Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, Shenzhen, 
      518055, China.
FAU - Zhang, Min
AU  - Zhang M
AUID- ORCID: 0000-0002-2225-8024
AD  - School of Electronic and Computer Engineering, Peking University, Shenzhen, 
      518055, China.
LA  - eng
GR  - GXWD20201231165807007-20200827122756001/Shenzhen Fundamental Research Program/
GR  - 62074008/National Natural Science Foundation of China/
GR  - 21977010/National Natural Science Foundation of China/
GR  - 2018YFA0902504/the Key Technologies Research and Development Program of China/
GR  - NCTIB2022HS1017/Innovation Projects of National Center of Technology Innovation 
      for Biopharmaceuticals/
PT  - Journal Article
DEP - 20231015
PL  - Germany
TA  - Adv Mater
JT  - Advanced materials (Deerfield Beach, Fla.)
JID - 9885358
RN  - 0 (Biomarkers)
RN  - 9007-49-2 (DNA)
RN  - 0 (DNA Probes)
RN  - 0 (Nanotubes, Carbon)
SB  - IM
MH  - Female
MH  - Humans
MH  - *Endometriosis
MH  - Biomarkers
MH  - DNA/analysis
MH  - DNA Probes
MH  - *Nanotubes, Carbon
MH  - *Biosensing Techniques/methods
MH  - Limit of Detection
OTO - NOTNLM
OT  - breast cancer
OT  - endometriosis
OT  - monkeypox virus
OT  - tetrahedral DNA nanostructure
OT  - transistor-based biosensors
EDAT- 2023/07/24 19:09
MHDA- 2024/02/12 18:42
CRDT- 2023/07/24 12:13
PHST- 2023/07/18 00:00 [revised]
PHST- 2023/05/03 00:00 [received]
PHST- 2024/02/12 18:42 [medline]
PHST- 2023/07/24 19:09 [pubmed]
PHST- 2023/07/24 12:13 [entrez]
AID - 10.1002/adma.202304119 [doi]
PST - ppublish
SO  - Adv Mater. 2023 Nov;35(46):e2304119. doi: 10.1002/adma.202304119. Epub 2023 Oct 
      15.