PMID- 37493452 OWN - NLM STAT- MEDLINE DCOM- 20230815 LR - 20230909 IS - 2373-9878 (Electronic) IS - 2373-9878 (Linking) VI - 9 IP - 8 DP - 2023 Aug 14 TI - Regeneration of Mechanically Enhanced Tissue-Engineered Cartilage Based on the Decalcified Bone Matrix Framework. PG - 4994-5005 LID - 10.1021/acsbiomaterials.3c00488 [doi] AB - Human decalcified bone matrix (HDBM) is a framework with a porous structure and good biocompatibility. Nevertheless, its oversized pores lead to massive cell loss when seeding chondrocytes directly over it. Gelatin (GT) is a type of protein obtained by partial hydrolysis of collagen. The GT scaffold can be prepared from the GT solution through freeze-drying. More importantly, the pore size of the GT scaffold can be controlled by optimizing the concentration of the GT solution. Similarly, when different concentrations of gelatin are combined with HDBM and then freeze-dried, the pore size of the HDBM can be modified to different degrees. In this study, the HDBM framework was modified with 0.3, 0.6, and 0.9%GT, resulting in an improved pore size and adhesion rate. Results showed that the HDBM framework with 0.6%GT (HDBM-0.6%GT) had an average pore size of 200 mum, which was more suitable for chondrocyte seeding. Additionally, our study validated that porcine decalcified bone matrix (PDBM) had a proper pore structure. Chondrocytes were in vitro seeded on the three frameworks for 4 weeks and then implanted in nude mice and autologous goats, respectively. The in vivo cartilage regeneration results showed that HDBM-0.6%GT and PDBM frameworks compensated for the oversized pores of the HDBM framework. Moreover, they showed successfully regenerated more mature cartilage tissue with a certain shape in animals. FAU - Yu, Mengyuan AU - Yu M AUID- ORCID: 0000-0001-9908-5320 AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. FAU - Song, Daiying AU - Song D AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China. AD - National Tissue Engineering Center of China, Shanghai 200241, PR China. AD - Research Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, Shandong 261021, PR China. FAU - Guo, Xueqiang AU - Guo X AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. FAU - Hu, Guanhuai AU - Hu G AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. FAU - Pei, Mengyu AU - Pei M AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. FAU - Fan, Zhenlin AU - Fan Z AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. FAU - Xi, Lingling AU - Xi L AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. FAU - Wen, Mengnan AU - Wen M AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. FAU - Ci, Zheng AU - Ci Z AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200430, PR China. FAU - Zhou, Guangdong AU - Zhou G AUID- ORCID: 0000-0003-2488-2733 AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China. AD - National Tissue Engineering Center of China, Shanghai 200241, PR China. FAU - Ren, Wenjie AU - Ren W AD - Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230726 PL - United States TA - ACS Biomater Sci Eng JT - ACS biomaterials science & engineering JID - 101654670 RN - 9000-70-8 (Gelatin) SB - IM MH - Mice MH - Swine MH - Humans MH - Animals MH - *Tissue Scaffolds/chemistry MH - *Bone Matrix MH - Gelatin/pharmacology/chemistry MH - Mice, Nude MH - Cartilage OTO - NOTNLM OT - cartilage injury OT - cartilage regeneration OT - decalcified bone matrix framework OT - gelatin OT - tissue engineering EDAT- 2023/07/26 13:07 MHDA- 2023/08/15 06:42 CRDT- 2023/07/26 09:24 PHST- 2023/08/15 06:42 [medline] PHST- 2023/07/26 13:07 [pubmed] PHST- 2023/07/26 09:24 [entrez] AID - 10.1021/acsbiomaterials.3c00488 [doi] PST - ppublish SO - ACS Biomater Sci Eng. 2023 Aug 14;9(8):4994-5005. doi: 10.1021/acsbiomaterials.3c00488. Epub 2023 Jul 26.