PMID- 37502906
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231113
DP  - 2023 Jul 19
TI  - Analysis of small EV proteomes reveals unique functional protein networks 
      regulated by VAP-A.
LID - 2023.07.18.549588 [pii]
LID - 10.1101/2023.07.18.549588 [doi]
AB  - Extracellular vesicles (EVs) influence cell phenotypes and functions via protein, 
      nucleic acid and lipid cargoes. EVs are heterogeneous, due to diverse biogenesis 
      mechanisms that remain poorly understood. Our previous study revealed that the 
      endoplasmic reticulum (ER) membrane contact site (MCS) linker protein VAP-A 
      drives biogenesis of a subset of RNA-enriched EVs. Here, we examine the protein 
      content of VAP-A-regulated EVs. Using label-free proteomics, we identified down- 
      and up-regulated proteins in sEVs purified from VAP-A knockdown (KD) colon cancer 
      cells. Gene set enrichment analysis (GSEA) of the data revealed protein classes 
      that are differentially sorted to SEVs dependent on VAP-A. STRING protein-protein 
      interaction network analysis of the RNA-binding protein (RBP) gene set identified 
      several RNA functional machineries that are downregulated in VAP-A KD EVs, 
      including ribosome, spliceosome, mRNA surveillance, and RNA transport proteins. 
      We also observed downregulation of other functionally interacting protein 
      networks, including cadherin-binding, unfolded protein binding, and ATP-dependent 
      proteins.
FAU - Barman, Bahnisikha
AU  - Barman B
AD  - Department of Cell and Developmental Biology, Vanderbilt University School of 
      Medicine, Nashville, Tennessee.
AD  - Vanderbilt Center for Extracellular Vesicle Research, Vanderbilt University, 
      Nashville, Tennessee.
FAU - Ramirez, Marisol
AU  - Ramirez M
AD  - Department of Biostatistics, Vanderbilt University Medical Center, Nashville, 
      Tennessee.
FAU - Dawson, T Renee
AU  - Dawson TR
AD  - Department of Cell and Developmental Biology, Vanderbilt University School of 
      Medicine, Nashville, Tennessee.
AD  - Vanderbilt Center for Extracellular Vesicle Research, Vanderbilt University, 
      Nashville, Tennessee.
FAU - Liu, Qi
AU  - Liu Q
AD  - Department of Biostatistics, Vanderbilt University Medical Center, Nashville, 
      Tennessee.
FAU - Weaver, Alissa M
AU  - Weaver AM
AD  - Department of Cell and Developmental Biology, Vanderbilt University School of 
      Medicine, Nashville, Tennessee.
AD  - Vanderbilt Center for Extracellular Vesicle Research, Vanderbilt University, 
      Nashville, Tennessee.
AD  - Department of Pathology, Microbiology and Immunology, Vanderbilt University 
      Medical Center, Nashville, Tennessee.
LA  - eng
GR  - P01 CA229123/CA/NCI NIH HHS/United States
PT  - Preprint
DEP - 20230719
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
UIN - Proteomics. 2023 Nov 5;:e2300099. PMID: 37926697
PMC - PMC10370063
COIS- Conflict of interest statement: The authors have declared no conflict of 
      interest.
EDAT- 2023/07/28 06:43
MHDA- 2023/07/28 06:44
PMCR- 2023/07/26
CRDT- 2023/07/28 04:24
PHST- 2023/07/28 06:43 [pubmed]
PHST- 2023/07/28 06:44 [medline]
PHST- 2023/07/28 04:24 [entrez]
PHST- 2023/07/26 00:00 [pmc-release]
AID - 2023.07.18.549588 [pii]
AID - 10.1101/2023.07.18.549588 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2023 Jul 19:2023.07.18.549588. doi: 
      10.1101/2023.07.18.549588.