PMID- 37506317
OWN - NLM
STAT- MEDLINE
DCOM- 20230811
LR  - 20230816
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 34
IP  - 13
DP  - 2023 Sep 6
TI  - TLR4-MyD88 signaling is involved in the spinal neurons during the full length of 
      recovery from transection of the motor branch of the femoral nerve in mice.
PG  - 655-663
LID - 10.1097/WNR.0000000000001939 [doi]
AB  - This study was designed to see the expression of toll-like receptor 4 (TLR4) and 
      downstream molecules including myeloid differentiation factor 88 (MyD88) and 
      interleukin 1-beta (IL-1beta) in the spinal cord as peripheral nerve injury recovered 
      in mice. We established a model of femoral nerve injury (FNI) in C57BL/6 mice by 
      transection of the motor branch of the femoral nerve, followed by retrograde 
      labeling to show the according motor neurons in the anterior horn of the spinal 
      cord pars lumbar. We observed the motor function recovery of the injured hind 
      limbs using behavioral tests. The expression of TLR4, MyD88, and IL-1beta was 
      examined by immunofluorescent staining and western blot. According to the 
      behavior test, the FNI animals fully recovered within 6-8 weeks. TLR4, MyD88, and 
      IL-1beta were expressed in the ventral horn of the spinal cord both at 72 h till 6 
      weeks after the femoral nerve transection surgery, and these proteins were mostly 
      co-localized with neurons. IL-1beta also tended to rise in the same surgery groups, 
      but more intimate with microglia surrounding nearby retrograde labeled neurons. 
      And western blot results were consistent with histological findings. The results 
      indicate that peripheral nerve injury may induce innate immune reactions of the 
      central neurons and critical signaling like TLR4/MyD88 in the spinal cord may 
      reflect the recovery of the injury. These findings suggest that peripheral nerve 
      injury triggered the TLR4/MyD88 signal in the soma of spinal neurons may be 
      involved in function and nerve restoration through neuron-glia crosstalk.
CI  - Copyright (c) 2023 The Author(s). Published by Wolters Kluwer Health, Inc.
FAU - Wei, Xiaoyan
AU  - Wei X
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
AD  - School of Medicine, Northwest University, Xi'an.
FAU - Ding, Yumeng
AU  - Ding Y
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
FAU - Wang, Lichao
AU  - Wang L
AD  - Naval Medical Center, Naval Medical University, Shanghai, China.
FAU - Zhang, Qixingmao
AU  - Zhang Q
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
FAU - Wang, Chunhuai
AU  - Wang C
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
FAU - Chen, Chen
AU  - Chen C
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
FAU - You, Siwei
AU  - You S
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
FAU - Wu, Mingmei
AU  - Wu M
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
FAU - Kuang, Fang
AU  - Kuang F
AD  - Department of Neurobiology, School of Basic Medicine, Fourth Military Medical 
      University.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230717
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Myeloid Differentiation Factor 88/metabolism
MH  - *Peripheral Nerve Injuries
MH  - Toll-Like Receptor 4/metabolism
MH  - Femoral Nerve/metabolism
MH  - Mice, Inbred C57BL
MH  - Motor Neurons/metabolism
PMC - PMC10399940
COIS- There are no conflicts of interest.
EDAT- 2023/07/28 19:11
MHDA- 2023/08/11 06:43
PMCR- 2023/08/03
CRDT- 2023/07/28 15:43
PHST- 2023/08/11 06:43 [medline]
PHST- 2023/07/28 19:11 [pubmed]
PHST- 2023/07/28 15:43 [entrez]
PHST- 2023/08/03 00:00 [pmc-release]
AID - 00001756-990000000-00141 [pii]
AID - 10.1097/WNR.0000000000001939 [doi]
PST - ppublish
SO  - Neuroreport. 2023 Sep 6;34(13):655-663. doi: 10.1097/WNR.0000000000001939. Epub 
      2023 Jul 17.