PMID- 37507874
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230731
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 12
IP  - 7
DP  - 2023 Jun 24
TI  - Antioxidants Improve the Proliferation and Efficacy of hUC-MSCs against 
      H(2)O(2)-Induced Senescence.
LID - 10.3390/antiox12071334 [doi]
LID - 1334
AB  - Human umbilical cord mesenchymal stem cells (hUC-MSCs) are broadly applied in 
      clinical treatment due to convenient accessibility, low immunogenicity, and the 
      absence of any ethical issues involved. However, the microenvironment of 
      inflammatory tissues may cause oxidative stress and induce senescence in 
      transplanted hUC-MSCs, which will further reduce the proliferation, migration 
      ability, and the final therapeutic effects of hUC-MSCs. Beta-nicotinamide 
      mononucleotide (NMN) and coenzyme Q10 (CoQ10) are famous antioxidants and 
      longevity medicines that could reduce intracellular reactive oxygen species 
      levels by different mechanisms. In this study, hUC-MSCs were treated in vitro 
      with NMN and CoQ10 to determine if they could reduce oxidative stress caused by 
      hydrogen peroxide (H(2)O(2)) and recover cell functions. The effects of NMN and 
      CoQ10 on the cell proliferation, the mRNA levels of the inflammatory cytokine 
      TNFalpha and the anti-inflammatory cytokine IL10, and the differentiation and cell 
      migration ability of hUC-MSCs before and after H(2)O(2) treatment were 
      investigated. The findings revealed that NMN and CoQ10 reduced H(2)O(2)-induced 
      senescence and increased hUC-MSCs' proliferation in the late phase as passage 12 
      and later. The TNFalpha mRNA level of hUC-MSCs induced by H(2)O(2) was significantly 
      decreased after antioxidant treatment. NMN and CoQ10 all reduced the adipogenic 
      differentiation ability of hUC-MSCs. CoQ10 improved the chondrogenic 
      differentiation ability of hUC-MSCs. Furthermore, NMN was found to significantly 
      enhance the migration ability of hUC-MSCs. Transcriptomic analysis revealed that 
      NMN and CoQ10 both increased DNA repair ability and cyclin expression and 
      downregulated TNF and IL-17 inflammatory signaling pathways, thereby contributing 
      to the proliferative promotion of senecent stem cells and resistance to oxidative 
      stress. These findings suggest that antioxidants can improve the survival and 
      efficacy of hUC-MSCs in stem cell therapy for inflammation-related diseases.
FAU - Zheng, Zhaojuan
AU  - Zheng Z
AUID- ORCID: 0009-0005-5782-7601
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Wang, Xia
AU  - Wang X
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Ouyang, Liming
AU  - Ouyang L
AUID- ORCID: 0000-0003-3507-7895
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Chen, Wenxia
AU  - Chen W
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Zhang, Lixin
AU  - Zhang L
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, Shanghai 200237, China.
FAU - Cao, Yulin
AU  - Cao Y
AD  - Beijing Tang Yi Hui Kang Biomedical Technology Co., Ltd., Beijing 100032, China.
LA  - eng
PT  - Journal Article
DEP - 20230624
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC10376626
OTO - NOTNLM
OT  - RNAseq
OT  - cell proliferation
OT  - coenzyme Q10
OT  - human umbilical cord mesenchymal stem cells (hUC-MSCs)
OT  - nicotinamide mononucleotide (NMN)
OT  - senescence
COIS- The authors declare that there is no conflict of interest regarding the 
      publication of this paper. Corresponding author Liming Ouyang has received 
      research grants from Beijing Tang Yi Hui Kang Biomedical Technology Co., Ltd.
EDAT- 2023/07/29 11:43
MHDA- 2023/07/29 11:44
PMCR- 2023/06/24
CRDT- 2023/07/29 01:03
PHST- 2023/04/15 00:00 [received]
PHST- 2023/06/12 00:00 [revised]
PHST- 2023/06/21 00:00 [accepted]
PHST- 2023/07/29 11:44 [medline]
PHST- 2023/07/29 11:43 [pubmed]
PHST- 2023/07/29 01:03 [entrez]
PHST- 2023/06/24 00:00 [pmc-release]
AID - antiox12071334 [pii]
AID - antioxidants-12-01334 [pii]
AID - 10.3390/antiox12071334 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2023 Jun 24;12(7):1334. doi: 10.3390/antiox12071334.