PMID- 37508331
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230731
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 12
IP  - 7
DP  - 2023 Jun 23
TI  - Cotton RSG2 Mediates Plant Resistance against Verticillium dahliae by miR482b 
      Regulation.
LID - 10.3390/biology12070898 [doi]
LID - 898
AB  - Cotton Verticillium wilt, mainly caused by Verticillium dahliae, has a serious 
      impact on the yield and quality of cotton fiber. Many microRNAs (miRNAs) have 
      been identified to participate in plant resistance to V. dahliae infection, but 
      the exploration of miRNA's function mechanism in plant defense is needed. Here, 
      we demonstrate that the ghr-miR482b-GhRSG2 module mediates cotton plant 
      resistance to V. dahliae infection. Based on the mRNA degradation data and GUS 
      fusion experiments, ghr-miR482b directedly bonds to GhRSG2 mRNA to lead to its 
      degradation. The knockdown and overexpression of ghr-miR482b through 
      virus-induced gene silencing strategies enhanced (decreased by 0.39-fold in 
      disease index compared with the control) and weakened (increased by 0.46-fold) 
      the plant resistance to V. dahliae, respectively. In addition, silencing GhRSG2 
      significantly increased (increased by 0.93-fold in disease index) the plant 
      sensitivity to V. dahliae compared with the control plants treated with empty 
      vector. The expression levels of two SA-related disease genes, GhPR1 and GhPR2, 
      significantly decreased in GhRSG2-silenced plants by 0.71 and 0.67 times, 
      respectively, and in ghr-miR482b-overexpressed (OX) plants by 0.59 and 0.75 
      times, respectively, compared with the control, whereas the expression levels of 
      GhPR1 and GhPR2 were significantly increased by 1.21 and 2.59 times, 
      respectively, in ghr-miR482b knockdown (KD) plants. In sum, the 
      ghr-miR482b-GhRSG2 module participates in the regulation of plant defense against 
      V. dahliae by inducing the expression of PR1 and PR2 genes.
FAU - Wu, Pan
AU  - Wu P
AD  - State Key Laboratory of Aridland Crop Science, College of Life Science and 
      Technology, Gansu Agricultural University, Lanzhou 730070, China.
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
AD  - Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, 
      Changji 831100, China.
FAU - Lu, Chengzhe
AU  - Lu C
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Wang, Bingting
AU  - Wang B
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Zhang, Feiyan
AU  - Zhang F
AD  - State Key Laboratory of Aridland Crop Science, College of Life Science and 
      Technology, Gansu Agricultural University, Lanzhou 730070, China.
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Shi, Linfang
AU  - Shi L
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Xu, Yunjiao
AU  - Xu Y
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
FAU - Chen, Aimin
AU  - Chen A
AD  - The Key Laboratory for the Creation of Cotton Varieties in the Northwest, 
      Ministry of Agriculture and Rural Affairs, Changji 831100, China.
FAU - Si, Huaijun
AU  - Si H
AUID- ORCID: 0000-0002-7288-2677
AD  - State Key Laboratory of Aridland Crop Science, College of Life Science and 
      Technology, Gansu Agricultural University, Lanzhou 730070, China.
FAU - Su, Junji
AU  - Su J
AD  - State Key Laboratory of Aridland Crop Science, College of Life Science and 
      Technology, Gansu Agricultural University, Lanzhou 730070, China.
AD  - Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, 
      Changji 831100, China.
FAU - Wu, Jiahe
AU  - Wu J
AD  - State Key Laboratory of Aridland Crop Science, College of Life Science and 
      Technology, Gansu Agricultural University, Lanzhou 730070, China.
AD  - State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese 
      Academy of Sciences, Beijing 100101, China.
LA  - eng
GR  - 2022D01E103/Natural Science Foundation of Xinjiang Uygur Autonomous Region/
GR  - 31971905/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230623
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC10376429
OTO - NOTNLM
OT  - GhRSG2
OT  - Gossypium hirsutum
OT  - Verticillium dahliae
OT  - ghr-miR482b
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/29 11:49
MHDA- 2023/07/29 11:50
PMCR- 2023/06/23
CRDT- 2023/07/29 01:06
PHST- 2023/05/13 00:00 [received]
PHST- 2023/06/16 00:00 [revised]
PHST- 2023/06/16 00:00 [accepted]
PHST- 2023/07/29 11:50 [medline]
PHST- 2023/07/29 11:49 [pubmed]
PHST- 2023/07/29 01:06 [entrez]
PHST- 2023/06/23 00:00 [pmc-release]
AID - biology12070898 [pii]
AID - biology-12-00898 [pii]
AID - 10.3390/biology12070898 [doi]
PST - epublish
SO  - Biology (Basel). 2023 Jun 23;12(7):898. doi: 10.3390/biology12070898.