PMID- 37509879
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230801
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 12
IP  - 14
DP  - 2023 Jul 22
TI  - Effectiveness of Bacteriophages against Biofilm-Forming Shiga-Toxigenic 
      Escherichia coli In Vitro and on Food-Contact Surfaces.
LID - 10.3390/foods12142787 [doi]
LID - 2787
AB  - (1) Background: Formation of biofilms on food-contact surfaces by Shiga-toxigenic 
      Escherichia coli (STEC) can pose a significant challenge to the food industry, 
      making conventional control methods insufficient. Targeted use of bacteriophages 
      to disrupt these biofilms could reduce this problem. Previously isolated and 
      characterized bacteriophages (n = 52) were evaluated against STEC biofilms in 
      vitro and on food-contact surfaces. (2) Methods: Phage treatments (9 logs PFU/mL) 
      in phosphate-buffered saline were used individually or as cocktails. Biofilms of 
      STEC (O157, O26, O45, O103, O111, O121, and O145) were formed in 96-well 
      micro-titer plates (7 logs CFU/mL; 24 h) or on stainless steel (SS) and 
      high-density polyethylene (HDPE) coupons (9 logs CFU/cm(2); 7 h), followed by 
      phage treatment. Biofilm disruption was measured in vitro at 0, 3, and 6 h as a 
      change in optical density (A(595)). Coupons were treated with STEC 
      serotype-specific phage-cocktails or a 21-phage cocktail (3 phages/serotype) for 
      0, 3, 6, and 16 h, and surviving STEC populations were enumerated. (3) Results: 
      Of the 52 phages, 77% showed STEC biofilm disruption in vitro. Serotype-specific 
      phage treatments reduced pathogen population within the biofilms by 1.9-4.1 and 
      2.3-5.6 logs CFU/cm(2), while the 21-phage cocktail reduced it by 4.0 and 4.8 
      logs CFU/cm(2) on SS and HDPE, respectively. (4) Conclusions: Bacteriophages can 
      be used to reduce STEC and their biofilms.
FAU - Jaroni, Divya
AU  - Jaroni D
AD  - Department of Animal and Food Sciences, and Food and Agricultural Products 
      Center, Oklahoma State University, N. Monroe Street, Stillwater, OK 74078, USA.
FAU - Litt, Pushpinder Kaur
AU  - Litt PK
AD  - Department of Animal and Food Sciences, and Food and Agricultural Products 
      Center, Oklahoma State University, N. Monroe Street, Stillwater, OK 74078, USA.
FAU - Bule, Punya
AU  - Bule P
AD  - Department of Animal and Food Sciences, and Food and Agricultural Products 
      Center, Oklahoma State University, N. Monroe Street, Stillwater, OK 74078, USA.
FAU - Rumbaugh, Kaylee
AU  - Rumbaugh K
AD  - Department of Animal and Food Sciences, and Food and Agricultural Products 
      Center, Oklahoma State University, N. Monroe Street, Stillwater, OK 74078, USA.
LA  - eng
PT  - Journal Article
DEP - 20230722
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC10378794
OTO - NOTNLM
OT  - Shiga-toxigenic E. coli
OT  - bacteriophages
OT  - biocontrol
OT  - biofilms
OT  - food-contact surfaces
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/29 11:43
MHDA- 2023/07/29 11:44
PMCR- 2023/07/22
CRDT- 2023/07/29 01:14
PHST- 2023/06/23 00:00 [received]
PHST- 2023/07/12 00:00 [revised]
PHST- 2023/07/15 00:00 [accepted]
PHST- 2023/07/29 11:44 [medline]
PHST- 2023/07/29 11:43 [pubmed]
PHST- 2023/07/29 01:14 [entrez]
PHST- 2023/07/22 00:00 [pmc-release]
AID - foods12142787 [pii]
AID - foods-12-02787 [pii]
AID - 10.3390/foods12142787 [doi]
PST - epublish
SO  - Foods. 2023 Jul 22;12(14):2787. doi: 10.3390/foods12142787.