PMID- 37510339
OWN - NLM
STAT- MEDLINE
DCOM- 20230809
LR  - 20240501
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 14
IP  - 7
DP  - 2023 Jul 12
TI  - Variations in NSP1 of Porcine Reproductive and Respiratory Syndrome Virus 
      Isolated in China from 1996 to 2022.
LID - 10.3390/genes14071435 [doi]
LID - 1435
AB  - Since its successful isolation in China in 1995, the porcine reproductive and 
      respiratory syndrome virus (PRRSV) has been mutating into highly pathogenic 
      strains by constantly changing pathogenicity and genetic makeup. In this study, 
      we investigated the prevalence and genetic variation of nonstructural protein 1 
      (NSP1) in PRRSV-2, the main strain prevalent in China. After formulating 
      hypotheses regarding the biology of the NSP1 protein, the nucleotide and amino 
      acid similarity of NSP1 were analyzed and compared in 193 PRRSV-2 strains. The 
      results showed that NSP1 has a stable hydrophobic protein with a molecular weight 
      of 43,060.76 Da. Although NSP1 lacked signal peptides, it could regulate host 
      cell signaling. Furthermore, NSP1 of different strains had high nucleotide 
      (79.6-100%) and amino acid similarity (78.6-100%). In the amino acid sequence 
      comparison of 15 representative strains of PRRSV-2, multiple amino acid 
      substitution sites were found in NSP1. Phylogenetic tree analysis showed that 
      lineages 1 and 8 had different evolutionary branches with long genetic distances. 
      This study lays the foundation for an in-depth understanding of the nature and 
      genetic variation of NSP1 and the development of a safe and effective vaccine in 
      the future.
FAU - Zhang, Zhiqing
AU  - Zhang Z
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Zhang, Hang
AU  - Zhang H
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Luo, Qin
AU  - Luo Q
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Zheng, Yajie
AU  - Zheng Y
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Kong, Weili
AU  - Kong W
AUID- ORCID: 0000-0002-9104-7730
AD  - Gladstone Institutes of Virology and Immunology, University of California, San 
      Francisco, CA 94158, USA.
FAU - Huang, Liangzong
AU  - Huang L
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
FAU - Zhao, Mengmeng
AU  - Zhao M
AUID- ORCID: 0000-0003-4143-3606
AD  - School of Life Science and Engineering, Foshan University, Foshan 528000, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230712
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (Nucleotides)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Nsp1a protein, Porcine Reproductive and Respiratory Syndrome Virus)
SB  - IM
MH  - Animals
MH  - Amino Acid Sequence
MH  - Nucleotides
MH  - Phylogeny
MH  - *Porcine Reproductive and Respiratory Syndrome/epidemiology/genetics
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Swine
MH  - *Viral Nonstructural Proteins/genetics
PMC - PMC10379836
OTO - NOTNLM
OT  - NSP1
OT  - porcine reproductive and respiratory syndrome
OT  - porcine reproductive and respiratory syndrome virus
OT  - variations
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/29 11:49
MHDA- 2023/07/31 11:41
PMCR- 2023/07/12
CRDT- 2023/07/29 01:17
PHST- 2023/06/11 00:00 [received]
PHST- 2023/07/03 00:00 [revised]
PHST- 2023/07/11 00:00 [accepted]
PHST- 2023/07/31 11:41 [medline]
PHST- 2023/07/29 11:49 [pubmed]
PHST- 2023/07/29 01:17 [entrez]
PHST- 2023/07/12 00:00 [pmc-release]
AID - genes14071435 [pii]
AID - genes-14-01435 [pii]
AID - 10.3390/genes14071435 [doi]
PST - epublish
SO  - Genes (Basel). 2023 Jul 12;14(7):1435. doi: 10.3390/genes14071435.