PMID- 37511286
OWN - NLM
STAT- MEDLINE
DCOM- 20230731
LR  - 20230801
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 14
DP  - 2023 Jul 16
TI  - Target Discovery of Matrine against PRRSV in Marc-145 Cells via Activity-Based 
      Protein Profiling.
LID - 10.3390/ijms241411526 [doi]
LID - 11526
AB  - Porcine reproductive and respiratory syndrome (PRRS) seriously endangers the 
      sustainable development of the pig industry. Our previous studies have shown that 
      matrine can resist porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection. This study aimed to explore the anti-PRRSV targets of matrine in 
      Marc-145 cells. Biotin-labeled matrine 1 and 2 were used as probes. MTT assay was 
      used to determine the maximum non-cytotoxic concentration (MNTC) of each probe in 
      Marc-145 cells. The anti-PRRSV activity of each probe was evaluated via MTT, qPCR 
      and Western blot, and its anti-inflammatory activity was evaluated via qPCR and 
      Western blot. The targets of matrine in Marc-145 cells were searched using 
      activity-based protein profiling (ABPP), and compared with the targets predicted 
      via network pharmacology for screening the potential targets of matrine against 
      PRRSV. The protein-protein interaction networks (PPI) of potential targets were 
      constructed using a network database and GO/KEGG enrichment analysis was 
      performed. ACAT1, ALB, HMOX1, HSPA8, HSP90AB1, PARP1 and STAT1 were identified as 
      potential targets of matrine, and their functions were related to antiviral 
      capacity and immunity. Matrine may play an anti-PRRSV role by directly acting on 
      ACAT1, ALB, HMOX1, HSPA8, HSP90AB1, PARP1 and STAT1.
FAU - Ling, Xiaoya
AU  - Ling X
AUID- ORCID: 0009-0005-3136-2703
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Cao, Zhigang
AU  - Cao Z
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Sun, Panpan
AU  - Sun P
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Zhang, Hua
AU  - Zhang H
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Sun, Yaogui
AU  - Sun Y
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Zhong, Jia
AU  - Zhong J
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Yin, Wei
AU  - Yin W
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Fan, Kuohai
AU  - Fan K
AUID- ORCID: 0000-0003-1333-7270
AD  - Laboratory Animal Center, Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Zheng, Xiaozhong
AU  - Zheng X
AD  - Centre for Inflammation Research, Queen's Medical Research Institute, The 
      University of Edinburgh, Edinburgh EH16 4TJ, UK.
FAU - Li, Hongquan
AU  - Li H
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
FAU - Sun, Na
AU  - Sun N
AD  - Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, 
      Shanxi Agricultural University, Jinzhong 030600, China.
LA  - eng
GR  - 32172904/National Natural Science Foundation of China/
GR  - 2022YFD1801101/National Key Research and Development Program/
GR  - 202102140601019/Key Research and Development Plan of Shanxi Province/
GR  - 202204051001021/the special fund for Science and Technology Innovation Teams of 
      Shanxi Province/
GR  - 2021L108/Scientific and Technological Innovation Programs of Higher Education 
      Institutions in Shanxi/
GR  - No. 20211331-16, No. 20211331-13/Shanxi "1331 Project"/
GR  - none/the earmarked fund for Modern Agro-industry Technology Research System/
PT  - Journal Article
DEP - 20230716
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Matrines)
RN  - 0 (Antiviral Agents)
SB  - IM
MH  - Animals
MH  - Swine
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Matrines
MH  - Cell Line
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - Antiviral Agents/pharmacology
MH  - Virus Replication
PMC - PMC10381006
OTO - NOTNLM
OT  - ABPP
OT  - PRRSV
OT  - drug targets
OT  - matrine
OT  - probe
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/29 11:52
MHDA- 2023/07/31 06:42
PMCR- 2023/07/16
CRDT- 2023/07/29 01:23
PHST- 2023/06/18 00:00 [received]
PHST- 2023/07/05 00:00 [revised]
PHST- 2023/07/13 00:00 [accepted]
PHST- 2023/07/31 06:42 [medline]
PHST- 2023/07/29 11:52 [pubmed]
PHST- 2023/07/29 01:23 [entrez]
PHST- 2023/07/16 00:00 [pmc-release]
AID - ijms241411526 [pii]
AID - ijms-24-11526 [pii]
AID - 10.3390/ijms241411526 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jul 16;24(14):11526. doi: 10.3390/ijms241411526.