PMID- 37511411
OWN - NLM
STAT- MEDLINE
DCOM- 20230731
LR  - 20230801
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 14
DP  - 2023 Jul 19
TI  - Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland 
      Hypofunction in a Mouse Model.
LID - 10.3390/ijms241411654 [doi]
LID - 11654
AB  - Radiotherapy and chemotherapy can impair salivary gland (SG) function, which 
      causes xerostomia and exacerbate other side effects of chemotherapy and oral 
      infection, reducing patients' quality of life. This animal study aimed to assess 
      the efficacy of electroacupuncture (EA) as a means of preventing xerostomia 
      induced by 5-fluorouracil (5-FU). A xerostomia mouse model was induced via four 
      tail vein injections of 5-FU (80 mg/kg/dose). EA was performed at LI4 and LI11 
      for 7 days. The pilocarpine-stimulated salivary flow rate (SFR) and salivary 
      glands weight (SGW) were recorded. Salivary immunoglobulin A (SIgA) and lysozyme 
      were determined via enzyme-linked immunosorbent assay (ELISA). SG was collected 
      for hematoxylin and eosin staining to measure acini number and acinar cell size. 
      Tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and aquaporin 5 (AQP5) 
      mRNA expressions in SG were quantified via RT-qPCR. 5-FU caused significant 
      decreases in SFR, SGW, SIgA, lysozyme, AQP5 expression, and acini number, while 
      TNF-alpha and IL-1beta expressions and acinar cell size were significantly increased. EA 
      treatment can prevent 5-FU damage to the salivary gland, while pilocarpine 
      treatment can only elevate SFR and AQP5 expression. These findings provide 
      significant evidence to support the use of EA as an alternative treatment for 
      chemotherapy-induced salivary gland hypofunction and xerostomia.
FAU - Nguyen, Thanh-Hien Vu
AU  - Nguyen TV
AUID- ORCID: 0009-0002-7280-0616
AD  - Graduate Institute of Acupuncture Science, China Medical University, Taichung 
      40402, Taiwan.
AD  - School of Dentistry, China Medical University, Taichung 40402, Taiwan.
FAU - Chiu, Kuo-Chou
AU  - Chiu KC
AD  - Division of Oral Diagnosis and Family Dentistry, Tri-Service General Hospital, 
      National Defense Medical Center, Taipei 11490, Taiwan.
FAU - Shih, Yin-Hwa
AU  - Shih YH
AUID- ORCID: 0000-0003-0893-4185
AD  - Department of Healthcare Administration, Asia University, Taichung 41354, Taiwan.
FAU - Liu, Chung-Ji
AU  - Liu CJ
AUID- ORCID: 0000-0002-0385-869X
AD  - Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei 
      10449, Taiwan.
FAU - Bao Quach, Tran Van
AU  - Bao Quach TV
AD  - School of Dentistry, China Medical University, Taichung 40402, Taiwan.
FAU - Hsia, Shih-Min
AU  - Hsia SM
AUID- ORCID: 0000-0003-2888-2618
AD  - School of Nutrition and Health Sciences, Taipei Medical University, Taipei 
      110301, Taiwan.
AD  - Nutrition Research Center, Taipei Medical University Hospital, Taipei 110301, 
      Taiwan.
FAU - Chen, Yi-Hung
AU  - Chen YH
AUID- ORCID: 0000-0002-8946-7939
AD  - Graduate Institute of Acupuncture Science, China Medical University, Taichung 
      40402, Taiwan.
AD  - Chinese Medicine Research Center, China Medical University, Taichung 40402, 
      Taiwan.
AD  - Department of Photonics and Communication Engineering, Asia University, Taichung 
      41354, Taiwan.
FAU - Shieh, Tzong-Ming
AU  - Shieh TM
AUID- ORCID: 0000-0003-1759-9120
AD  - School of Dentistry, China Medical University, Taichung 40402, Taiwan.
LA  - eng
GR  - MOST 111-2314-B-039-027-MY3 and NSTC 111-2811-B-039-034./Ministry of Science and 
      Technology/
GR  - CMU111-S-36 and CMU111-MF-38/China Medical University/
PT  - Journal Article
DEP - 20230719
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - EC 3.2.1.17 (Muramidase)
RN  - 01MI4Q9DI3 (Pilocarpine)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - U3P01618RT (Fluorouracil)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Immunoglobulin A, Secretory)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Muramidase/genetics
MH  - Pilocarpine
MH  - *Electroacupuncture
MH  - Quality of Life
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - Salivary Glands
MH  - *Xerostomia/chemically induced/therapy
MH  - Fluorouracil/adverse effects
MH  - *Antineoplastic Agents
MH  - Immunoglobulin A, Secretory
PMC - PMC10380826
OTO - NOTNLM
OT  - 5-fluorouracil
OT  - acupuncture
OT  - chemotherapy
OT  - inflammation
OT  - salivary hypofunction
OT  - xerostomia
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/29 11:50
MHDA- 2023/07/31 06:42
PMCR- 2023/07/19
CRDT- 2023/07/29 01:24
PHST- 2023/06/07 00:00 [received]
PHST- 2023/07/10 00:00 [revised]
PHST- 2023/07/14 00:00 [accepted]
PHST- 2023/07/31 06:42 [medline]
PHST- 2023/07/29 11:50 [pubmed]
PHST- 2023/07/29 01:24 [entrez]
PHST- 2023/07/19 00:00 [pmc-release]
AID - ijms241411654 [pii]
AID - ijms-24-11654 [pii]
AID - 10.3390/ijms241411654 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Jul 19;24(14):11654. doi: 10.3390/ijms241411654.