PMID- 37512356
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230801
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 16
IP  - 14
DP  - 2023 Jul 19
TI  - Lipid Corona Formation on Micro- and Nanoplastic Particles Modulates Uptake and 
      Toxicity in A549 Cells.
LID - 10.3390/ma16145082 [doi]
LID - 5082
AB  - Plastic waste is a global issue leaving no continents unaffected. In the 
      environment, ultraviolet radiation and shear forces in water and land contribute 
      to generating micro- and nanoplastic particles (MNPP), which organisms can easily 
      take up. Plastic particles enter the human food chain, and the accumulation of 
      particles within the human body is expected. Crossing epithelial barriers and 
      cellular uptake of MNPP involves the interaction of plastic particles with 
      lipids. To this end, we generated unilamellar vesicles from POPC 
      (1-palmitoyl-2-oleoyl-glycero-3-phosphocholine) and POPS 
      (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine) and incubated them with 
      pristine, carboxylated, or aminated polystyrene spheres (about 1 microm in diameter) 
      to generate lipid coronas around the particles. Lipid coronas enhanced the 
      average particle sizes and partially changed the MNPP zeta potential and 
      polydispersity. In addition, lipid coronas led to significantly enhanced uptake 
      of MNPP particles but not their cytotoxicity, as determined by flow cytometry. 
      Finally, adding proteins to lipid corona nanoparticles further modified MNPP 
      uptake by reducing the uptake kinetics, especially in pristine and carboxylated 
      plastic samples. In conclusion, our study demonstrates for the first time the 
      impact of different types of lipids on differently charged MNPP particles and the 
      biological consequences of such modifications to better understand the potential 
      hazards of plastic exposure.
FAU - Dorsch, Anna Daniela
AU  - Dorsch AD
AD  - ZIK plasmatis, Leibniz Institute for Plasma Science and Technology (INP), 
      Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
FAU - da Silva Brito, Walison Augusto
AU  - da Silva Brito WA
AD  - ZIK plasmatis, Leibniz Institute for Plasma Science and Technology (INP), 
      Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
AD  - Department of General Pathology, State University of Londrina, Rodovia Celso 
      Garcia Cid, Londrina 86057-970, Brazil.
FAU - Delcea, Mihaela
AU  - Delcea M
AD  - Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Str. 4, 
      17487 Greifswald, Germany.
FAU - Wende, Kristian
AU  - Wende K
AUID- ORCID: 0000-0001-5217-0683
AD  - ZIK plasmatis, Leibniz Institute for Plasma Science and Technology (INP), 
      Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
FAU - Bekeschus, Sander
AU  - Bekeschus S
AUID- ORCID: 0000-0002-8773-8862
AD  - ZIK plasmatis, Leibniz Institute for Plasma Science and Technology (INP), 
      Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
AD  - Clinic and Policlinic for Dermatology and Venerology, Rostock University Medical 
      Center, Strempelstr. 13, 18057 Rostock, Germany.
LA  - eng
GR  - 03Z22D511/Federal Ministry of Education and Research/
PT  - Journal Article
DEP - 20230719
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC10386368
OTO - NOTNLM
OT  - A549 cells
OT  - dynamic light scattering
OT  - microplastic
OT  - surface plasmon resonance
OT  - unilamellar vesicles
COIS- The authors have no conflict of interest to declare.
EDAT- 2023/07/29 11:50
MHDA- 2023/07/29 11:51
PMCR- 2023/07/19
CRDT- 2023/07/29 01:29
PHST- 2023/06/23 00:00 [received]
PHST- 2023/07/15 00:00 [revised]
PHST- 2023/07/17 00:00 [accepted]
PHST- 2023/07/29 11:51 [medline]
PHST- 2023/07/29 11:50 [pubmed]
PHST- 2023/07/29 01:29 [entrez]
PHST- 2023/07/19 00:00 [pmc-release]
AID - ma16145082 [pii]
AID - materials-16-05082 [pii]
AID - 10.3390/ma16145082 [doi]
PST - epublish
SO  - Materials (Basel). 2023 Jul 19;16(14):5082. doi: 10.3390/ma16145082.