PMID- 37512820
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231102
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 11
IP  - 7
DP  - 2023 Jun 24
TI  - Design of a Multi-Epitope Vaccine against Tuberculosis from Mycobacterium 
      tuberculosis PE_PGRS49 and PE_PGRS56 Proteins by Reverse Vaccinology.
LID - 10.3390/microorganisms11071647 [doi]
LID - 1647
AB  - Tuberculosis is a disease caused by Mycobacterium tuberculosis, representing the 
      second leading cause of death by an infectious agent worldwide. The available 
      vaccine against this disease has insufficient coverage and variable efficacy, 
      accounting for a high number of cases worldwide. In fact, an estimated third of 
      the world's population has a latent infection. Therefore, developing new vaccines 
      is crucial to preventing it. In this study, the highly antigenic PE_PGRS49 and 
      PE_PGRS56 proteins were analyzed. These proteins were used for predicting T- and 
      B-cell epitopes and for human leukocyte antigen (HLA) protein binding efficiency. 
      Epitopes GGAGGNGSLSS, FAGAGGQGGLGG, GIGGGTQSATGLG (PE_PGRS49), and GTGWNGGKGDTG 
      (PE_PGRS56) were selected based on their best physicochemical, antigenic, 
      non-allergenic, and non-toxic properties and coupled to HLA I and HLA II 
      structures for in silico assays. A construct with an adjuvant (RS09) plus each 
      epitope joined by GPGPG linkers was designed, and the stability of the 
      HLA-coupled construct was further evaluated by molecular dynamics simulations. 
      Although experimental and in vivo studies are still necessary to ensure its 
      protective effect against the disease, this study shows that the vaccine 
      construct is dynamically stable and potentially effective against tuberculosis.
FAU - Ruaro-Moreno, Maritriny
AU  - Ruaro-Moreno M
AUID- ORCID: 0000-0002-2066-4133
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
AD  - Posgrado en Biomedicina y Biotecnologia Molecular, Escuela Nacional de Ciencias 
      Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Monterrubio-Lopez, Gloria Paulina
AU  - Monterrubio-Lopez GP
AUID- ORCID: 0000-0002-2651-3977
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Reyes-Gastellou, Abraham
AU  - Reyes-Gastellou A
AUID- ORCID: 0000-0003-2676-4183
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Castelan-Vega, Juan Arturo
AU  - Castelan-Vega JA
AUID- ORCID: 0000-0003-1102-2101
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
AD  - Posgrado en Biomedicina y Biotecnologia Molecular, Escuela Nacional de Ciencias 
      Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Jimenez-Alberto, Alicia
AU  - Jimenez-Alberto A
AUID- ORCID: 0000-0002-7127-0791
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
AD  - Posgrado en Biomedicina y Biotecnologia Molecular, Escuela Nacional de Ciencias 
      Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Aparicio-Ozores, Gerardo
AU  - Aparicio-Ozores G
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
AD  - Posgrado en Biomedicina y Biotecnologia Molecular, Escuela Nacional de Ciencias 
      Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Delgadillo-Gutierrez, Karen
AU  - Delgadillo-Gutierrez K
AUID- ORCID: 0000-0002-5353-8192
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Gonzalez-Y-Merchand, Jorge Alberto
AU  - Gonzalez-Y-Merchand JA
AD  - Posgrado en Biomedicina y Biotecnologia Molecular, Escuela Nacional de Ciencias 
      Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11340, Mexico.
FAU - Ribas-Aparicio, Rosa Maria
AU  - Ribas-Aparicio RM
AUID- ORCID: 0000-0002-1141-2794
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (IPN), Mexico City 11340, Mexico.
AD  - Posgrado en Biomedicina y Biotecnologia Molecular, Escuela Nacional de Ciencias 
      Biologicas, Instituto Politecnico Nacional (IPN), Mexico City 11340, Mexico.
LA  - eng
GR  - SIP-20221624, SIP-20232106, SIP-20221962, SIP-20231999, SIP-20221987, 
      SIP-20232094, SIP-20221948 and SIP-20232124/Instituto Politecnico Nacional/
PT  - Journal Article
DEP - 20230624
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10385543
OTO - NOTNLM
OT  - B- and T-cell epitope prediction
OT  - computational vaccinology
OT  - tuberculosis
OT  - vaccine
COIS- The authors declare no conflict of interest.
EDAT- 2023/07/29 11:47
MHDA- 2023/07/29 11:48
PMCR- 2023/06/24
CRDT- 2023/07/29 01:32
PHST- 2023/05/08 00:00 [received]
PHST- 2023/06/12 00:00 [revised]
PHST- 2023/06/14 00:00 [accepted]
PHST- 2023/07/29 11:48 [medline]
PHST- 2023/07/29 11:47 [pubmed]
PHST- 2023/07/29 01:32 [entrez]
PHST- 2023/06/24 00:00 [pmc-release]
AID - microorganisms11071647 [pii]
AID - microorganisms-11-01647 [pii]
AID - 10.3390/microorganisms11071647 [doi]
PST - epublish
SO  - Microorganisms. 2023 Jun 24;11(7):1647. doi: 10.3390/microorganisms11071647.