PMID- 37516879
OWN - NLM
STAT- MEDLINE
DCOM- 20230731
LR  - 20230801
IS  - 1749-799X (Electronic)
IS  - 1749-799X (Linking)
VI  - 18
IP  - 1
DP  - 2023 Jul 29
TI  - Exosomes promote hFOB1.19 proliferation and differentiation via LINC00520.
PG  - 546
LID - 10.1186/s13018-023-04021-y [doi]
LID - 546
AB  - BACKGROUND: Osteoporosis remains a significant clinical challenge worldwide. 
      Recent studies have shown that exosomes stimulate bone regeneration. Thus, it is 
      worthwhile to explore whether exosomes could be a useful therapeutic strategy for 
      osteoporosis. The purpose of this study was to investigate the effects of 
      exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs) on 
      osteoblast proliferation and differentiation. METHODS: Exosomes were isolated 
      from hucMSCs. Bioinformatics analysis was performed to identify the 
      differentially expressed lncRNAs in myeloma-derived mesenchymal stem cells. 
      Plasmids encoding LINC00520 or short hairpin RNA of LINC00520 were transfected 
      into hucMSCs and then exosomes were isolated. After human osteoblasts hFOB1.19 
      were exposed to the obtained exosomes, cell survival, cell cycle, apoptosis and 
      calcium deposits of hFOB1.19 cell were detected by MTT, 7-aminoactinomycin D, 
      Annexin V-FITC/propidium iodide and Alizarin red staining, respectively. RESULTS: 
      In hFOB1.19 cells, 10 x 10(9)/mL hucMSC-derived exosomes inhibited cell 
      proliferation, arrested cell cycle, and promoted apoptosis, while hucMSCs or 
      1 x 10(9)/mL exosomes promoted cell proliferation, accelerated cell cycle, and 
      promoted calcium deposits and the expression of OCN, RUNX2, collagen I and ALP. 
      In hFOB1.19 cells, exosomes from hucMSCs with LINC00520 knockdown reduced the 
      survival and calcium deposits, arrested the cell cycle, and enhanced the 
      apoptosis, while exosomes from hucMSCs overexpressing LINC00520 enhance the 
      proliferation and calcium deposits and accelerated the cell cycle. CONCLUSIONS: 
      LINC00520 functions as a modulator of calcium deposits, and exosomes derived from 
      hucMSCs overexpressing LINC00520 might be a novel therapeutic approach for 
      osteoporosis.
CI  - (c) 2023. The Author(s).
FAU - Wu, Jin
AU  - Wu J
AD  - Medical School of Chinese PLA, Chinese PLA General Hospital, No. 28 Fuxing Road, 
      Haidian District, Beijing, 100853, China.
AD  - Department of Orthopedics, National Clinical Research Center for Orthopedics, 
      Sports Medicine and Rehabilitation, Chinese PLA General Hospital, Beijing, 
      100853, China.
AD  - Department of Orthopedics, The 909th Hospital, School of Medicine, Xiamen 
      University, Zhangzhou, 363000, China.
FAU - Zhang, Licheng
AU  - Zhang L
AD  - Medical School of Chinese PLA, Chinese PLA General Hospital, No. 28 Fuxing Road, 
      Haidian District, Beijing, 100853, China. zhanglcheng218@126.com.
AD  - Department of Orthopedics, National Clinical Research Center for Orthopedics, 
      Sports Medicine and Rehabilitation, Chinese PLA General Hospital, Beijing, 
      100853, China. zhanglcheng218@126.com.
FAU - Liu, Hui
AU  - Liu H
AD  - Department of Orthopedics, The 909th Hospital, School of Medicine, Xiamen 
      University, Zhangzhou, 363000, China.
FAU - Zhang, Jinhui
AU  - Zhang J
AD  - Department of Orthopedics, The 909th Hospital, School of Medicine, Xiamen 
      University, Zhangzhou, 363000, China.
FAU - Tang, Peifu
AU  - Tang P
AD  - Medical School of Chinese PLA, Chinese PLA General Hospital, No. 28 Fuxing Road, 
      Haidian District, Beijing, 100853, China. pftang301@163.com.
AD  - Department of Orthopedics, National Clinical Research Center for Orthopedics, 
      Sports Medicine and Rehabilitation, Chinese PLA General Hospital, Beijing, 
      100853, China. pftang301@163.com.
LA  - eng
PT  - Journal Article
DEP - 20230729
PL  - England
TA  - J Orthop Surg Res
JT  - Journal of orthopaedic surgery and research
JID - 101265112
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Humans
MH  - *Calcium
MH  - *Exosomes/genetics
MH  - Cell Cycle/genetics
MH  - Cell Differentiation/genetics
MH  - Cell Proliferation/genetics
PMC - PMC10387216
OTO - NOTNLM
OT  - Human umbilical cord mesenchymal stem cell-derived exosomes
OT  - LINC00520
OT  - Osteoblast
OT  - Osteoporosis
COIS- No competing interests were involved in this study.
EDAT- 2023/07/30 01:06
MHDA- 2023/07/31 11:42
PMCR- 2023/07/29
CRDT- 2023/07/29 23:27
PHST- 2023/01/13 00:00 [received]
PHST- 2023/07/15 00:00 [accepted]
PHST- 2023/07/31 11:42 [medline]
PHST- 2023/07/30 01:06 [pubmed]
PHST- 2023/07/29 23:27 [entrez]
PHST- 2023/07/29 00:00 [pmc-release]
AID - 10.1186/s13018-023-04021-y [pii]
AID - 4021 [pii]
AID - 10.1186/s13018-023-04021-y [doi]
PST - epublish
SO  - J Orthop Surg Res. 2023 Jul 29;18(1):546. doi: 10.1186/s13018-023-04021-y.