PMID- 37517511 OWN - NLM STAT- MEDLINE DCOM- 20230911 LR - 20230912 IS - 1872-8359 (Electronic) IS - 0167-7012 (Linking) VI - 212 DP - 2023 Sep TI - Application of FT-IR Spectroscopy for Mycobacterium abscessus complex subspecies differentiation. PG - 106792 LID - S0167-7012(23)00126-4 [pii] LID - 10.1016/j.mimet.2023.106792 [doi] AB - Mycobacterium abscessus complex (MABSC) subspecies differentiation improves patients' therapy and outcome. Fourier-Transform-Infrared Spectroscopy (FT-IRS) was applied for subspecies discrimination of 15 strains on different media: Lowenstein-Jensen showed the best resolution power; Linear Discriminant Analysis model differentiated M. abscessus susbsp. abscessus from M. abscessus subsp. massiliense. FT-IRS has a potential role in rapidly MABSC subspecies identification. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - Curtoni, Antonio AU - Curtoni A AD - Microbiology and Virology Unit, University Hospital Citta della Salute e della Scienza di Torino, Turin, Italy; Department of Public Health and Paediatrics, University of Turin, Turin, Italy. FAU - Cordovana, Miriam AU - Cordovana M AD - Bruker Daltonics GmbH & Co. KG, Bremen, Germany. FAU - Bondi, Alessandro AU - Bondi A AD - Microbiology and Virology Unit, University Hospital Citta della Salute e della Scienza di Torino, Turin, Italy; Department of Public Health and Paediatrics, University of Turin, Turin, Italy. Electronic address: alessandro.bondi@unito.it. FAU - Scaiola, Francesca AU - Scaiola F AD - Department of Public Health and Paediatrics, University of Turin, Turin, Italy. FAU - Criscione, Giulia AU - Criscione G AD - Department of Public Health and Paediatrics, University of Turin, Turin, Italy. FAU - Ghibaudo, Davide AU - Ghibaudo D AD - Department of Public Health and Paediatrics, University of Turin, Turin, Italy. FAU - Pastrone, Lisa AU - Pastrone L AD - Department of Public Health and Paediatrics, University of Turin, Turin, Italy. FAU - Zanotto, Elisa AU - Zanotto E AD - Microbiology and Virology Unit, University Hospital Citta della Salute e della Scienza di Torino, Turin, Italy. FAU - Camaggi, Anna AU - Camaggi A AD - Microbiology and Virology Unit, Azienda Ospedaliero-Universitaria Maggiore della Carita di Novara, Novara, Italy. FAU - Caroppo, Maria Simona AU - Caroppo MS AD - Microbiology and Virology Unit, Azienda Ospedaliero-Universitaria Maggiore della Carita di Novara, Novara, Italy. FAU - Kostrzewa, Markus AU - Kostrzewa M AD - Department of Public Health and Paediatrics, University of Turin, Turin, Italy. FAU - Cavallo, Rossana AU - Cavallo R AD - Microbiology and Virology Unit, University Hospital Citta della Salute e della Scienza di Torino, Turin, Italy; Department of Public Health and Paediatrics, University of Turin, Turin, Italy. FAU - Costa, Cristina AU - Costa C AD - Microbiology and Virology Unit, University Hospital Citta della Salute e della Scienza di Torino, Turin, Italy; Department of Public Health and Paediatrics, University of Turin, Turin, Italy. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230728 PL - Netherlands TA - J Microbiol Methods JT - Journal of microbiological methods JID - 8306883 SB - IM MH - Humans MH - *Mycobacterium abscessus MH - Spectroscopy, Fourier Transform Infrared OTO - NOTNLM OT - FT-IR spectroscopy OT - IR biotyper OT - LPA OT - Mycobacterium abscessus complex OT - Subtyping COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Miriam Cordovana and Markus Kostrzewa are employed at the Bruker Daltonics GmbH & Co. KG. All other authors report no potential conflicts of interest. EDAT- 2023/07/31 00:41 MHDA- 2023/09/11 06:42 CRDT- 2023/07/30 19:14 PHST- 2023/06/12 00:00 [received] PHST- 2023/07/27 00:00 [revised] PHST- 2023/07/27 00:00 [accepted] PHST- 2023/09/11 06:42 [medline] PHST- 2023/07/31 00:41 [pubmed] PHST- 2023/07/30 19:14 [entrez] AID - S0167-7012(23)00126-4 [pii] AID - 10.1016/j.mimet.2023.106792 [doi] PST - ppublish SO - J Microbiol Methods. 2023 Sep;212:106792. doi: 10.1016/j.mimet.2023.106792. Epub 2023 Jul 28.