PMID- 37517821
OWN - NLM
STAT- MEDLINE
DCOM- 20230801
LR  - 20230801
IS  - 1875-5364 (Electronic)
IS  - 1875-5364 (Linking)
VI  - 21
IP  - 7
DP  - 2023 Jul
TI  - Ginsenoside-Rg1 combined with a conditioned medium from induced neuron-like 
      hUCMSCs alleviated the apoptosis in a cell model of ALS through regulating the 
      NF-kappaB/Bcl-2 pathway.
PG  - 540-550
LID - S1875-5364(23)60445-5 [pii]
LID - 10.1016/S1875-5364(23)60445-5 [doi]
AB  - Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease 
      affecting both upper and lower motor neurons in the brain and spinal cord. One 
      important aspect of ALS pathogenesis is superoxide dismutase 1 (SOD1) 
      mutant-mediated mitochondrial toxicity, leading to apoptosis in neurons. This 
      study aimed to evaluate the neural protective synergistic effects of ginsenosides 
      Rg1 (G-Rg1) and conditioned medium (CM) on a mutational SOD1 cell model, and to 
      explore the underlying mechanisms. We found that the contents of nerve growth 
      factor, glial cell line-derived neurotrophic factor, and brain-derived 
      neurotrophic factor significantly increased in CM after human umbilical cord 
      mesenchymal stem cells (hUCMSCs) were exposed to neuron differentiation reagents 
      for seven days. CM or G-Rg1 decreased the apoptotic rate of SOD1(G93A)-NSC34 
      cells to a certain extent, but their combination brought about the least 
      apoptosis, compared with CM or G-Rg1 alone. Further research showed that the 
      anti-apoptotic protein Bcl-2 was upregulated in all the treatment groups. 
      Proteins associated with mitochondrial apoptotic pathways, such as Bax, caspase 9 
      (Cas-9), and cytochrome c (Cyt c), were downregulated. Furthermore, CM or G-Rg1 
      also inhibited the activation of the nuclear factor-kappa B (NF-kappaB) signaling 
      pathway by reducing the phosphorylation of p65 and IkappaBalpha. CM/G-Rg1 or their 
      combination also reduced the apoptotic rate induced by betulinic acid (BetA), an 
      agonist of the NF-kappaB signaling pathway. In summary, the combination of CM and 
      G-Rg1 effectively reduced the apoptosis of SOD1(G93A)-NSC34 cells through 
      suppressing the NF-kappaB/Bcl-2 signaling pathway (Fig. 1 is a graphical 
      representation of the abstract).
CI  - Copyright (c) 2023 China Pharmaceutical University. Published by Elsevier B.V. All 
      rights reserved.
FAU - Huang, Yu
AU  - Huang Y
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Labortory of Stem Cell Clinical 
      Research, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, 
      China.
FAU - Yang, Huili
AU  - Yang H
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Department of Neurology, Guangdong 
      Provincial Hospital of Chinese Medicine, Guangzhou 510006, China.
FAU - Yang, Biying
AU  - Yang B
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Department of Neurology, Guangdong 
      Provincial Hospital of Chinese Medicine, Guangzhou 510006, China.
FAU - Zheng, Yu
AU  - Zheng Y
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Department of Neurology, Guangdong 
      Provincial Hospital of Chinese Medicine, Guangzhou 510006, China.
FAU - Hou, Xiaomei
AU  - Hou X
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Department of Neurology, Guangdong 
      Provincial Hospital of Chinese Medicine, Guangzhou 510006, China.
FAU - Chen, Guiling
AU  - Chen G
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Labortory of Stem Cell Clinical 
      Research, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, 
      China.
FAU - Zhang, Wenqi
AU  - Zhang W
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Department of Neurology, Guangdong 
      Provincial Hospital of Chinese Medicine, Guangzhou 510006, China.
FAU - Zeng, Xiang
AU  - Zeng X
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Labortory of Stem Cell Clinical 
      Research, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, 
      China.
FAU - DU, Baoxin
AU  - DU B
AD  - Labortory of Stem Cell Clinical Reaearch, The Second Clinical College of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, The Second Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Labortory of 
      Stem Cell Biology and Chinese Medicine, Guangdong Provincial Academy of Chinese 
      Medical Sciences, Guangzhou 510006, China; Department of Neurology, Guangdong 
      Provincial Hospital of Chinese Medicine, Guangzhou 510006, China. Electronic 
      address: dbx.dr@163.com.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Nat Med
JT  - Chinese journal of natural medicines
JID - 101504416
RN  - 0 (NF-kappa B)
RN  - PJ788634QY (ginsenoside Rg1)
RN  - 0 (Ginsenosides)
RN  - 0 (Culture Media, Conditioned)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
SB  - IM
MH  - Humans
MH  - NF-kappa B/genetics/metabolism
MH  - *Ginsenosides/pharmacology
MH  - *Amyotrophic Lateral Sclerosis/drug therapy/genetics
MH  - Culture Media, Conditioned/pharmacology
MH  - Superoxide Dismutase-1
MH  - *Neurodegenerative Diseases
MH  - Neurons/metabolism
MH  - Apoptosis
OTO - NOTNLM
OT  - Amyotrophic lateral sclerosis
OT  - Apoptosis
OT  - Conditioned medium
OT  - Ginsenoside-Rg1
OT  - Umbilical cord mesenchymal stem cells
EDAT- 2023/07/31 00:41
MHDA- 2023/08/01 06:44
CRDT- 2023/07/30 20:55
PHST- 2023/02/06 00:00 [received]
PHST- 2023/08/01 06:44 [medline]
PHST- 2023/07/31 00:41 [pubmed]
PHST- 2023/07/30 20:55 [entrez]
AID - S1875-5364(23)60445-5 [pii]
AID - 10.1016/S1875-5364(23)60445-5 [doi]
PST - ppublish
SO  - Chin J Nat Med. 2023 Jul;21(7):540-550. doi: 10.1016/S1875-5364(23)60445-5.