PMID- 37522242
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231202
IS  - 1386-6346 (Print)
IS  - 1386-6346 (Linking)
VI  - 53
IP  - 12
DP  - 2023 Dec
TI  - Dried blood spot-based host genome analysis technique targeting pathological 
      associations with hepatitis B: Development and clinical application in the 
      Cambodian population.
PG  - 1147-1155
LID - 10.1111/hepr.13949 [doi]
AB  - AIM: Reports of patients with hepatitis B have highlighted associations between 
      polymorphisms in the human leukocyte antigen (HLA)-DPB1, CXCL13, and CXCR5 genes 
      and disease pathology. Owing to its potential to contribute to the development of 
      new diagnostic and therapeutic methods, we aimed to establish a reliable host 
      genome analysis technique that can be used in countries with inadequate 
      infrastructure. METHOD: We compared multiple commercially available kits for 
      dried blood spot (DBS)-based sample collection to develop a basic DBS-based host 
      genome analysis technique. We then collected blood samples from Cambodian 
      patients with hepatitis  B and performed single-nucleotide polymorphism 
      genotyping and HLA allele typing by the DBS system. RESULT: We were able to 
      perform single-nucleotide polymorphism genotyping and HLA allele typing with host 
      DNA samples obtained using a combination of a HemaSpot filter paper-based device 
      and a SMITEST((R)) EX-R&D DNA extraction kit. The accuracy of genotyping using 
      samples obtained by this method was not inferior to one using samples obtained by 
      venipuncture. In the Cambodian population, significant associations of 
      HLA-DPB1*04:01 with protection against chronic hepatitis B virus (HBV) infection, 
      and HLA-DPB1*05:01 and HLA-DPB1*13:01 with susceptibility to chronic HBV 
      infection were identified. CONCLUSION: Based on the DBS system, we clarified the 
      associations of HLA-DPB1 alleles with chronic HBV infection in the Cambodian 
      population for the first time. Because the DBS is a low-cost, durable, 
      transportable, and easy-to-handle modality, genetic analysis based on the DBS 
      system is a feasible strategy for obtaining a deeper understanding of HBV 
      epidemiology, especially in middle- or low-income countries.
CI  - (c) 2023 Japan Society of Hepatology.
FAU - Setoyama, Hiroko
AU  - Setoyama H
AD  - Hepatitis Information Center, The Research Center for Hepatitis and Immunology, 
      National Center for Global Health and Medicine, Ichikawa, Japan.
AD  - Department of Gastroenterology and Hepatology, Graduate School of Medical 
      Sciences, Kumamoto University, Kumamoto, Japan.
FAU - Nishida, Nao
AU  - Nishida N
AD  - Genome Medical Science Project, National Center for Global Health and Medicine, 
      Ichikawa, Japan.
FAU - Nagashima, Shintaro
AU  - Nagashima S
AD  - Department of Epidemiology, Infectious Disease Control and Prevention, Graduate 
      School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Ko, Ko
AU  - Ko K
AD  - Department of Epidemiology, Infectious Disease Control and Prevention, Graduate 
      School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Yamazoe, Taiji
AU  - Yamazoe T
AD  - Hepatitis Information Center, The Research Center for Hepatitis and Immunology, 
      National Center for Global Health and Medicine, Ichikawa, Japan.
FAU - Tanaka, Yasuhito
AU  - Tanaka Y
AUID- ORCID: 0000-0002-2473-6966
AD  - Department of Gastroenterology and Hepatology, Graduate School of Medical 
      Sciences, Kumamoto University, Kumamoto, Japan.
FAU - Mizokami, Masashi
AU  - Mizokami M
AD  - Genome Medical Science Project, National Center for Global Health and Medicine, 
      Ichikawa, Japan.
FAU - Tanaka, Junko
AU  - Tanaka J
AUID- ORCID: 0000-0002-5669-4051
AD  - Department of Epidemiology, Infectious Disease Control and Prevention, Graduate 
      School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Kanto, Tatsuya
AU  - Kanto T
AD  - Hepatitis Information Center, The Research Center for Hepatitis and Immunology, 
      National Center for Global Health and Medicine, Ichikawa, Japan.
LA  - eng
GR  - MHLW Policy Research for Hepatitis Measures Program/
GR  - National Center for Global Health and Medicine Intramural Research Fund/
PT  - Journal Article
DEP - 20230808
PL  - Netherlands
TA  - Hepatol Res
JT  - Hepatology research : the official journal of the Japan Society of Hepatology
JID - 9711801
OTO - NOTNLM
OT  - Cambodia
OT  - HLA-DP
OT  - dried blood spot testing
OT  - hepatitis B
OT  - single-nucleotide polymorphisms
EDAT- 2023/07/31 06:43
MHDA- 2023/07/31 06:44
CRDT- 2023/07/31 05:28
PHST- 2023/07/21 00:00 [revised]
PHST- 2023/05/30 00:00 [received]
PHST- 2023/07/28 00:00 [accepted]
PHST- 2023/07/31 06:44 [medline]
PHST- 2023/07/31 06:43 [pubmed]
PHST- 2023/07/31 05:28 [entrez]
AID - 10.1111/hepr.13949 [doi]
PST - ppublish
SO  - Hepatol Res. 2023 Dec;53(12):1147-1155. doi: 10.1111/hepr.13949. Epub 2023 Aug 8.