PMID- 37522313
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20240515
IS  - 1532-2750 (Electronic)
IS  - 1098-612X (Print)
IS  - 1098-612X (Linking)
VI  - 25
IP  - 7
DP  - 2023 Jul
TI  - Quantitative bioimaging of copper in frozen liver specimens from cats using laser 
      ablation-inductively coupled plasma-mass spectrometry.
PG  - 1098612X231186919
LID - 10.1177/1098612X231186919 [doi]
LID - 1098612X231186919
AB  - OBJECTIVES: The aim of this study was to assess laser ablation-inductively 
      coupled plasma-mass spectrometry (LA-ICP-MS) as a tool for measuring 
      concentrations and determining accumulation of copper in frozen liver specimens 
      from cats. METHODS: Six frozen liver specimens were evaluated by qualitative 
      copper staining and quantitative flame atomic absorption spectroscopy. Tissue 
      specimens were cryo-sectioned and quantitative bioimaging of copper was performed 
      using LA-ICP-MS. Results were compared with those obtained using conventional 
      methods. RESULTS: Of the six specimens, only one showed positive staining for 
      copper with rhodanine. Using flame atomic absorption spectroscopy (FAAS), one 
      specimen showed a deficient copper level (<100 microg/g dry weight), two specimens 
      had copper within the reference interval (RI; 150-180 microg/g) and three specimens 
      had copper concentrations above the RI. Bioimaging from LA-ICP-MS showed 
      inhomogeneous distribution of hepatic copper. The areas with dense copper 
      accumulation were represented as hotspots in the liver specimens. Hepatic copper 
      quantification by LA-ICP-MS correlated well with copper quantified by FAAS 
      (r = 0.96, P = 0.002). CONCLUSIONS AND RELEVANCE: Our findings suggest that 
      quantitative bioimaging by LA-ICP-MS could be used to demonstrate the 
      distribution and concentration of copper in frozen liver specimens from cats. The 
      distribution of copper in these specimens was inhomogeneous with dense 
      accumulation represented as hotspots on tissue sections. A positive correlation 
      of hepatic copper concentrations determined by LA-ICP-MS and FAAS was found. 
      Further studies to establish an RI for hepatic copper using this technique and to 
      further determine its clinical utility are warranted.
FAU - Yamkate, Punyamanee
AU  - Yamkate P
AUID- ORCID: 0000-0002-4464-644X
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School 
      of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College 
      Station, TX, USA.
AD  - Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn 
      University, Bangkok, Thailand.
FAU - Funke, Sabrina
AU  - Funke S
AD  - Institute for Inorganic and Analytical Chemistry, University of Munster, Munster, 
      Germany.
FAU - Steiger, Katja
AU  - Steiger K
AD  - Institute of Pathology, School of Medicine, Technical University Munich, Munich, 
      Germany.
FAU - Gold, Randi M
AU  - Gold RM
AUID- ORCID: 0000-0003-1226-7282
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School 
      of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College 
      Station, TX, USA.
FAU - Lidbury, Jonathan A
AU  - Lidbury JA
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School 
      of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College 
      Station, TX, USA.
FAU - Karst, Uwe
AU  - Karst U
AD  - Institute for Inorganic and Analytical Chemistry, University of Munster, Munster, 
      Germany.
FAU - Steiner, Joerg M
AU  - Steiner JM
AUID- ORCID: 0000-0003-3336-2086
AD  - Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School 
      of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College 
      Station, TX, USA.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Feline Med Surg
JT  - Journal of feline medicine and surgery
JID - 100897329
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Cats
MH  - Animals
MH  - *Copper
MH  - Mass Spectrometry/veterinary/methods
MH  - Liver/chemistry
MH  - *Laser Therapy/methods/veterinary
MH  - Spectrum Analysis/veterinary
PMC - PMC10812054
OTO - NOTNLM
OT  - Hepatic copper
OT  - LA-ICP-MS
OT  - atomic absorption spectroscopy
OT  - copper staining
OT  - heavy metal
OT  - laser ablation-inductively coupled plasma-mass spectrometry
OT  - liver
OT  - quantitative bioimaging
COIS- The authors declared no potential conflicts of interest with respect to the 
      research, authorship, and/or publication of this article.
EDAT- 2023/07/31 06:43
MHDA- 2023/10/23 00:43
PMCR- 2023/07/31
CRDT- 2023/07/31 05:43
PHST- 2023/10/23 00:43 [medline]
PHST- 2023/07/31 06:43 [pubmed]
PHST- 2023/07/31 05:43 [entrez]
PHST- 2023/07/31 00:00 [pmc-release]
AID - 10.1177_1098612X231186919 [pii]
AID - 10.1177/1098612X231186919 [doi]
PST - ppublish
SO  - J Feline Med Surg. 2023 Jul;25(7):1098612X231186919. doi: 
      10.1177/1098612X231186919.