PMID- 37522533
OWN - NLM
STAT- MEDLINE
DCOM- 20230801
LR  - 20230801
IS  - 1940-4344 (Electronic)
IS  - 1940-4344 (Linking)
VI  - 25
IP  - 6
DP  - 2023
TI  - Isolation, Characterization, and Antioxidant Activity of Melanin from Auricularia 
      auricula (Agaricomycetes).
PG  - 55-73
LID - 10.1615/IntJMedMushrooms.2023048271 [doi]
AB  - The cell wall of Auricularia auricula fruit bodies is extremely tough, making it 
      difficult to dissolve the melanin using the traditional preparation method. To 
      investigate the efficient preparation of melanin and its resistance to oxidative 
      stress, this paper first used ultrasound-assisted alkaline cellulase to optimize 
      the optimal wall-breaking parameters through a Box-Behnken design based on a 
      single-factor experiment. After optimization, the yield of melanin from A. 
      auricula reached 3.201 +/- 0.018%. Then, different types and different proportions 
      of deep eutectic solvents (DES) were used for further extraction. When choline 
      chloride and urea were selected and the ratio was 1:2, the melanin yield was up 
      to 25.99% +/- 2.36%. Scanning electron microscope (SEM) images showed that the 
      melanin was amorphous mass with no crystal structure. X-ray photoelectron 
      spectroscopy (XPS) analysis revealed that the melanin was mainly composed of C 
      (5.38%), O (15.69%) and N (30.29%), as was the typical composition of eumelanin. 
      The melanin had a concentration-dependent relationship with both ABTS+ and 
      hydroxyl radical scavenging ability; at the concentration of 0.5 mg/mL, it 
      significantly prolonged Caenorhabditis elegans survival under hydrogen peroxide 
      and methyl viologen stress and increased the glutathione level and enzyme (total 
      superoxide dismutase and catalase) activities in vivo compared with the negative 
      control (P < 0.05), indicating that the melanin enhances oxidative stress 
      resistance in C. elegans.
FAU - Shi, Qianwen
AU  - Shi Q
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
FAU - Yang, Zeen
AU  - Yang Z
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
FAU - Fan, Renhui
AU  - Fan R
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
FAU - Chu, Jialei
AU  - Chu J
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
FAU - Fang, Chenlu
AU  - Fang C
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
FAU - Zhang, Yusi
AU  - Zhang Y
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
FAU - Shi, Wenting
AU  - Shi W
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
FAU - Zhang, Yongjun
AU  - Zhang Y
AD  - College of Life Sciences, Key Laboratory of Specialty Agri-Product Quality and 
      Hazard Controlling Technology of Zhejiang Province, China Jiliang University, 
      Hangzhou, Zhejiang Province, 310018, P.R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Med Mushrooms
JT  - International journal of medicinal mushrooms
JID - 100886202
RN  - 0 (Antioxidants)
RN  - 0 (Melanins)
RN  - Auricularia auricula-judae
SB  - IM
MH  - Animals
MH  - *Antioxidants/chemistry
MH  - Melanins/chemistry
MH  - Caenorhabditis elegans
MH  - *Basidiomycota/chemistry
EDAT- 2023/07/31 13:08
MHDA- 2023/08/01 06:44
CRDT- 2023/07/31 07:53
PHST- 2023/08/01 06:44 [medline]
PHST- 2023/07/31 13:08 [pubmed]
PHST- 2023/07/31 07:53 [entrez]
AID - 6614f7f003dea2ca,325d4622512ed50f [pii]
AID - 10.1615/IntJMedMushrooms.2023048271 [doi]
PST - ppublish
SO  - Int J Med Mushrooms. 2023;25(6):55-73. doi: 10.1615/IntJMedMushrooms.2023048271.