PMID- 37526330
OWN - NLM
STAT- Publisher
LR  - 20231013
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 120
IP  - 11
DP  - 2023 Nov
TI  - Intracellular accumulation of c-di-GMP and its regulation on self-flocculation of 
      the bacterial cells of Zymomonas mobilis.
PG  - 3234-3243
LID - 10.1002/bit.28513 [doi]
AB  - Zymomonas mobilis is an emerging chassis for being engineered to produce bulk 
      products due to its unique glycolysis through the Entner-Doudoroff pathway with 
      less ATP produced for lower biomass accumulation and higher product yield. When 
      self-flocculated, the bacterial cells are more productive, since they can 
      self-immobilize within bioreactors for high density, and are more tolerant to 
      stresses for higher product titers, but this morphology needs to be controlled 
      properly to avoid internal mass transfer limitation associated with their strong 
      self-flocculation. Herewith we explored the regulation of cyclic diguanosine 
      monophosphate (c-di-GMP) on self-flocculation of the bacterial cells through 
      activating cellulose biosynthesis. While ZMO1365 and ZMO0919 with GGDEF domains 
      for diguanylate cyclase activity catalyze c-di-GMP biosynthesis, ZMO1487 with an 
      EAL domain for phosphodiesterase activity catalyzes c-di-GMP degradation, but 
      ZMO1055 and ZMO0401 contain the dual domains with phosphodiesterase activity 
      predominated. Since c-di-GMP is synthesized from GTP, the intracellular 
      accumulation of this signal molecule through deactivating phosphodiesterase 
      activity is preferred for activating cellulose biosynthesis to flocculate the 
      bacterial cells, because such a strategy exerts less perturbance on intracellular 
      processes regulated by GTP. These discoveries are significant for not only 
      engineering unicellular Z. mobilis strains with the self-flocculating morphology 
      to boost production but also understanding mechanism underlying c-di-GMP 
      biosynthesis and degradation in the bacterium.
CI  - (c) 2023 Wiley Periodicals LLC.
FAU - Li, Kai
AU  - Li K
AD  - State Key Laboratory of Microbial Metabolism, Joint International Research 
      Laboratory of Metabolic & Developmental Science, School of Life Science and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Xia, Juan
AU  - Xia J
AD  - State Key Laboratory of Microbial Metabolism, Joint International Research 
      Laboratory of Metabolic & Developmental Science, School of Life Science and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Liu, Chen-Guang
AU  - Liu CG
AUID- ORCID: 0000-0003-0343-6304
AD  - State Key Laboratory of Microbial Metabolism, Joint International Research 
      Laboratory of Metabolic & Developmental Science, School of Life Science and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Zhao, Xin-Qing
AU  - Zhao XQ
AD  - State Key Laboratory of Microbial Metabolism, Joint International Research 
      Laboratory of Metabolic & Developmental Science, School of Life Science and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Bai, Feng-Wu
AU  - Bai FW
AUID- ORCID: 0000-0003-1431-4839
AD  - State Key Laboratory of Microbial Metabolism, Joint International Research 
      Laboratory of Metabolic & Developmental Science, School of Life Science and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
LA  - eng
GR  - National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20230801
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
SB  - IM
OTO - NOTNLM
OT  - Zymomonas mobilis
OT  - c-di-GMP
OT  - chassis
OT  - industrial production
OT  - microbial cell factories
OT  - self-flocculation
EDAT- 2023/08/01 13:11
MHDA- 2023/08/01 13:11
CRDT- 2023/08/01 08:13
PHST- 2023/06/26 00:00 [revised]
PHST- 2023/03/27 00:00 [received]
PHST- 2023/07/17 00:00 [accepted]
PHST- 2023/08/01 13:11 [pubmed]
PHST- 2023/08/01 13:11 [medline]
PHST- 2023/08/01 08:13 [entrez]
AID - 10.1002/bit.28513 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2023 Nov;120(11):3234-3243. doi: 10.1002/bit.28513. Epub 2023 
      Aug 1.