PMID- 37528389 OWN - NLM STAT- MEDLINE DCOM- 20230803 LR - 20231123 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 19 IP - 1 DP - 2023 Aug 2 TI - Production of a chimeric porcine reproductive and respiratory syndrome virus (PRRSV)-2 vaccine using a lab-scale packed-bed bioreactor CelCradle. PG - 105 LID - 10.1186/s12917-023-03659-4 [doi] LID - 105 AB - BACKGROUND: We developed a MARC-145 cell culture and porcine reproductive and respiratory syndrome (PRRS) vaccine production using a novel CelCradle bioreactor. CelCradle is a packed-bed bioreactor capable of both batch and perfusion culture, and the operating parameters are easy to optimize. RESULTS: In this study, CelCradle reached a maximum cell density of 8.94 x 10(5) cells/mL at 5 days post-seeding when seeded at 8.60 x 10(4) cells/mL (doubling time = 35.52 h). Inoculation of PRRS vaccine candidate, K418DM1.1, was performed at a multiplicity of infection (MOI) of 0.01 at 5 days post-seeding, which resulted in a high viral titer of 2.04 x 10(8) TCID(50)/mL and total viral load of 1.02 x 10(11) TCID(50)/500 mL at 2 days post-infection (dpi). The multilayer cultivation system, BioFactory culture, yielded a higher doubling time (37.14 h) and lower viral titer (i.e., 8.15 x 10(7) TCID(50)/mL) compared to the CelCradle culture. Thus, the culture medium productivity of the CelCradle culture was 2-fold higher than that of the BioFactory culture. In the animal experiment, the CelCradle-produced vaccine induced high levels of neutralizing antibodies and effectively protected pigs against homologous challenge, as shown by the significantly lower levels of viremia at 1- and 7-days post-challenge (dpc) compared to the non-vaccinated pigs. CONCLUSIONS: Overall, this study demonstrates that the CelCradle system is an economical platform for PRRS vaccine production. CI - (c) 2023. The Author(s). FAU - Choi, Hwi-Yeon AU - Choi HY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Choi, Jong-Chul AU - Choi JC AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Kang, Yeong-Lim AU - Kang YL AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Ahn, So-Hyeun AU - Ahn SH AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Lee, Sang-Won AU - Lee SW AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Park, Seung-Yong AU - Park SY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Song, Chang-Seon AU - Song CS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Choi, In-Soo AU - Choi IS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. FAU - Lee, Joong-Bok AU - Lee JB AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. virus@konkuk.ac.kr. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea. virus@konkuk.ac.kr. LA - eng GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 818027-2/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ PT - Journal Article DEP - 20230802 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 RN - 0 (Antibodies, Viral) RN - 0 (Viral Vaccines) RN - 0 (Vaccines, Attenuated) SB - IM EIN - BMC Vet Res. 2023 Aug 9;19(1):115. PMID: 37559121 MH - Swine MH - Animals MH - *Porcine respiratory and reproductive syndrome virus MH - *Porcine Reproductive and Respiratory Syndrome/prevention & control MH - Antibodies, Viral MH - *Viral Vaccines MH - Vaccines, Attenuated MH - *Swine Diseases PMC - PMC10394776 OTO - NOTNLM OT - Bioreactor OT - CelCradle OT - Packed Bed OT - Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) OT - Vaccine efficacy OT - Vaccine production COIS- The authors declare that they have no competing interests. EDAT- 2023/08/02 01:07 MHDA- 2023/08/03 06:43 PMCR- 2023/08/02 CRDT- 2023/08/01 23:37 PHST- 2021/07/22 00:00 [received] PHST- 2023/07/14 00:00 [accepted] PHST- 2023/08/03 06:43 [medline] PHST- 2023/08/02 01:07 [pubmed] PHST- 2023/08/01 23:37 [entrez] PHST- 2023/08/02 00:00 [pmc-release] AID - 10.1186/s12917-023-03659-4 [pii] AID - 3659 [pii] AID - 10.1186/s12917-023-03659-4 [doi] PST - epublish SO - BMC Vet Res. 2023 Aug 2;19(1):105. doi: 10.1186/s12917-023-03659-4.