PMID- 37529178
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230803
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 10
DP  - 2023
TI  - Immunomodulatory effects of canine mesenchymal stem cells in an experimental 
      atopic dermatitis model.
PG  - 1201382
LID - 10.3389/fvets.2023.1201382 [doi]
LID - 1201382
AB  - Mesenchymal stem cells (MSCs) have the potential to differentiate into 
      multi-lineage cells, suggesting their future applicability in regenerative 
      medicine and biotechnology. The immunomodulatory properties of MSCs make them a 
      promising replacement therapy in various fields of animal research including in 
      canine atopic dermatitis (AD), a skin disease with 10-15% prevalence. We 
      investigated the immunomodulatory effects of MSCs in an experimental canine AD 
      model induced by Dermatophagoides farinae extract ointment. Canine adipose 
      tissue-derived MSCs (cAT-MSCs) were differentiated into mesodermal cell lineages 
      at the third passage. Alterations in immunomodulatory factors in control, AD, and 
      MSC-treated AD groups were evaluated using flow cytometric analysis, 
      enzyme-linked immunosorbent assay, and quantitative reverse transcription PCR. In 
      the MSC-treated AD group, the number of eosinophils decreased, and the number of 
      regulatory T cells (Tregs) increased compared to those in the AD group. In 
      addition, the immunoglobulin E (IgE) and prostaglandin E(2) levels were reduced 
      in the MSC-treated AD group compared to those in the AD group. Furthermore, the 
      filaggrin, vascular endothelial growth factor, and interleukin-5 gene expression 
      levels were relatively higher in the MSC-treated AD group than in the AD group, 
      however, not significantly. cAT-MSCs exerted immunomodulatory effects in an AD 
      canine model via a rebalancing of type-1 and -2 T helper cells that correlated 
      with increased levels of Tregs, IgE, and various cytokines.
CI  - Copyright (c) 2023 Kang, Gu, Byeon, Hyun, Lee and Yang.
FAU - Kang, Seok-Jin
AU  - Kang SJ
AD  - Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, 
      Republic of Korea.
FAU - Gu, Na-Yeon
AU  - Gu NY
AD  - Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, 
      Republic of Korea.
FAU - Byeon, Jeong Su
AU  - Byeon JS
AD  - Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, 
      Republic of Korea.
FAU - Hyun, Bang-Hun
AU  - Hyun BH
AD  - Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, 
      Republic of Korea.
FAU - Lee, Jienny
AU  - Lee J
AD  - Division of Regenerative Medicine Safety Management, Department of Chronic 
      Disease Convergence Research, Korea National Institute of Health, Korea Disease 
      Control and Prevention Agency, Cheongju, Republic of Korea.
FAU - Yang, Dong-Kun
AU  - Yang DK
AD  - Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20230713
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC10390254
OTO - NOTNLM
OT  - MSCs
OT  - atopic dermatitis
OT  - canine
OT  - dermatophagoides farina extract
OT  - immunomodulatory effect
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/08/02 06:43
MHDA- 2023/08/02 06:44
PMCR- 2023/01/01
CRDT- 2023/08/02 03:51
PHST- 2023/04/06 00:00 [received]
PHST- 2023/06/27 00:00 [accepted]
PHST- 2023/08/02 06:44 [medline]
PHST- 2023/08/02 06:43 [pubmed]
PHST- 2023/08/02 03:51 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2023.1201382 [doi]
PST - epublish
SO  - Front Vet Sci. 2023 Jul 13;10:1201382. doi: 10.3389/fvets.2023.1201382. 
      eCollection 2023.