PMID- 37531267
OWN - NLM
STAT- MEDLINE
DCOM- 20230907
LR  - 20231225
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 43
IP  - 9
DP  - 2023 Sep 27
TI  - Involvement of DPP3 in modulating oncological features and oxidative stress 
      response in esophageal squamous cell carcinoma.
LID - 10.1042/BSR20222472 [doi]
LID - BSR20222472
AB  - Resistance to therapy in esophageal squamous cell carcinoma (ESCC) is a critical 
      clinical problem and identification of novel therapeutic targets is highly 
      warranted. Dipeptidyl peptidase III (DPP3) is a zinc-dependent aminopeptidase and 
      functions in the terminal stages of the protein turnover. Several studies have 
      reported overexpression and oncogenic functions of DPP3 in numerous malignancies. 
      The present study aimed to determine the expression pattern and functional role 
      of DPP3 in ESCC. DPP3 expression was assessed in normal and tumor tissues using 
      quantitative real-time (qRT)-PCR and corroborated with ESCC gene expression 
      datasets from Gene Expression Omnibus (GEO) and The cancer genome atlas (TCGA). 
      DPP3 stable knockdown was performed in ESCC cells by shRNA and its effect on cell 
      proliferation, migration, cell cycle, apoptosis, and activation of nuclear factor 
      erythroid 2-related factor 2 (NRF2) pathway was assessed. The results suggested 
      that DPP3 is overexpressed in ESCC and its knockdown leads to reduced 
      proliferation, increased apoptosis, and inhibited migration of ESCC cells. 
      Additionally, DPP3 knockdown leads to down-regulation of the NRF2 pathway 
      proteins, such as NRF2, G6PD, and NQO1 along with increased sensitivity toward 
      oxidative stress-induced cell death and chemotherapy. Conclusively, these results 
      demonstrate critical role of DPP3 in ESCC and DPP3/NRF2 axis may serve as an 
      attractive therapeutic target against chemoresistance in this malignancy.
CI  - (c) 2023 The Author(s).
FAU - Arora, Mohit
AU  - Arora M
AUID- ORCID: 0000-0002-7417-5036
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Kumari, Sarita
AU  - Kumari S
AUID- ORCID: 0000-0002-9566-2933
AD  - Laboratory Oncology Unit, Dr. BRA-IRCH, All India Institute of Medical Sciences, 
      New Delhi, India.
FAU - Kadian, Lokesh
AU  - Kadian L
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Anupa, Geethadevi
AU  - Anupa G
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Singh, Jay
AU  - Singh J
AD  - Laboratory Oncology Unit, Dr. BRA-IRCH, All India Institute of Medical Sciences, 
      New Delhi, India.
FAU - Kumar, Anurag
AU  - Kumar A
AUID- ORCID: 0000-0002-5283-1821
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Verma, Deepika
AU  - Verma D
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Pramanik, Raja
AU  - Pramanik R
AD  - Department of Medical Oncology, All India Institute of Medical Sciences, New 
      Delhi, India.
FAU - Kumar, Sunil
AU  - Kumar S
AD  - Department of Surgical Oncology, All India Institute of Medical Sciences, New 
      Delhi, India.
FAU - Yadav, Rajni
AU  - Yadav R
AD  - Department of Pathology, All India Institute of Medical Sciences, New Delhi, 
      India.
FAU - Chopra, Anita
AU  - Chopra A
AUID- ORCID: 0000-0002-0238-8702
AD  - Laboratory Oncology Unit, Dr. BRA-IRCH, All India Institute of Medical Sciences, 
      New Delhi, India.
FAU - Chauhan, Shyam S
AU  - Chauhan SS
AUID- ORCID: 0000-0002-4796-5171
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 
      India.
LA  - eng
GR  - IA/CPHI/17/1/503333/WTDBT_/DBT-Wellcome Trust India Alliance/India
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (NF-E2-Related Factor 2)
RN  - EC 3.4.14.4 (DPP3 protein, human)
RN  - EC 3.4.14.- (Dipeptidyl-Peptidases and Tripeptidyl-Peptidases)
SB  - IM
MH  - Humans
MH  - *Esophageal Squamous Cell Carcinoma/pathology
MH  - *Esophageal Neoplasms/pathology
MH  - NF-E2-Related Factor 2/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Oxidative Stress
MH  - Cell Movement/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/genetics
PMC - PMC10500228
OTO - NOTNLM
OT  - Dipeptidyl peptidase
OT  - Nrf2
OT  - esophageal cancer
OT  - esophageal squamous cell carcinoma
OT  - oxidative stress
COIS- The authors declare that there are no competing interests associated with the 
      manuscript. The present study was approved by Institute Ethics Committee and 
      Institutional Biosafety Committee, All India Institute of Medical Sciences, New 
      Delhi, India.
EDAT- 2023/08/02 19:15
MHDA- 2023/09/07 06:42
PMCR- 2023/09/06
CRDT- 2023/08/02 12:52
PHST- 2022/12/07 00:00 [received]
PHST- 2023/07/13 00:00 [revised]
PHST- 2023/08/02 00:00 [accepted]
PHST- 2023/09/07 06:42 [medline]
PHST- 2023/08/02 19:15 [pubmed]
PHST- 2023/08/02 12:52 [entrez]
PHST- 2023/09/06 00:00 [pmc-release]
AID - 233346 [pii]
AID - BSR20222472 [pii]
AID - 10.1042/BSR20222472 [doi]
PST - ppublish
SO  - Biosci Rep. 2023 Sep 27;43(9):BSR20222472. doi: 10.1042/BSR20222472.