PMID- 37532719
OWN - NLM
STAT- MEDLINE
DCOM- 20230804
LR  - 20230805
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 14
IP  - 1
DP  - 2023 Aug 2
TI  - BIK1 protein homeostasis is maintained by the interplay of different ubiquitin 
      ligases in immune signaling.
PG  - 4624
LID - 10.1038/s41467-023-40364-0 [doi]
LID - 4624
AB  - Pathogen-associated molecular patterns (PAMPs) trigger plant innate immunity that 
      acts as the first line of inducible defense against pathogen infection. A 
      receptor-like cytoplasmic kinase BOTRYTIS-INDUCED KINASE 1 (BIK1) functions as a 
      signaling hub immediately downstream of multiple pattern recognition receptors 
      (PRRs). It is known that PLANT U-BOX PROTEIN 25 (PUB25) and PUB26 ubiquitinate 
      BIK1 and mediate BIK1 degradation. However, how BIK1 homeostasis is maintained is 
      not fully understood. Here, we show that two closely related ubiquitin ligases, 
      RING DOMAIN LIGASE 1 (RGLG1) and RGLG2, preferentially associate with the 
      hypo-phosphorylated BIK1 and promote the association of BIK1 with the co-receptor 
      for several PRRs, BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1). PUB25 interacts with 
      RGLG2 and mediates its degradation. In turn, RGLG2 represses the ubiquitin ligase 
      activity of PUB25. RGLG1/2 suppress PUB25-mediated BIK1 degradation, promote BIK1 
      protein accumulation, and positively regulate immune signaling in a ubiquitin 
      ligase activity-dependent manner. Our work reveals how BIK1 homeostasis is 
      maintained by the interplay of different ubiquitin ligases.
CI  - (c) 2023. The Author(s).
FAU - Bai, Jiaojiao
AU  - Bai J
AUID- ORCID: 0000-0002-8919-8329
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
AD  - Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai 
      Jiao Tong University, Shanghai, 200240, China.
AD  - College of Pharmacy and Life Science, Jiujiang University, Jiujiang, Jiangxi, 
      332000, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Zhou, Yuanyuan
AU  - Zhou Y
AUID- ORCID: 0000-0001-5688-9804
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
AD  - Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai 
      Jiao Tong University, Shanghai, 200240, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Sun, Jianhang
AU  - Sun J
AUID- ORCID: 0000-0002-2730-8721
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Chen, Kexin
AU  - Chen K
AUID- ORCID: 0000-0001-6371-4092
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Han, Yufang
AU  - Han Y
AUID- ORCID: 0009-0001-3065-0560
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Wang, Ranran
AU  - Wang R
AUID- ORCID: 0000-0002-0839-5372
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Zou, Yanmin
AU  - Zou Y
AUID- ORCID: 0000-0002-4609-6277
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
FAU - Du, Mingshuo
AU  - Du M
AUID- ORCID: 0000-0002-7384-1391
AD  - State Key Laboratory of Plant Genomics, Center for Agricultural Resources 
      Research, Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences, Shijiazhuang, Hebei, 050021, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Lu, Dongping
AU  - Lu D
AUID- ORCID: 0000-0002-4888-0532
AD  - Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai 
      Jiao Tong University, Shanghai, 200240, China. dplu@sjziam.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230802
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - 0 (Arabidopsis Proteins)
RN  - EC 6.- (Ligases)
RN  - 0 (Ubiquitin)
RN  - EC 2.7.- (Protein Kinases)
RN  - 0 (Plant Proteins)
RN  - 0 (Receptors, Pattern Recognition)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.11.1 (BIK1 protein, Arabidopsis)
RN  - EC 2.3.2.27 (RGLG2 protein, Arabidopsis)
SB  - IM
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Botrytis/metabolism
MH  - *Arabidopsis Proteins/genetics/metabolism
MH  - Phosphorylation
MH  - *Arabidopsis/metabolism
MH  - Ligases/metabolism
MH  - Ubiquitin/metabolism
MH  - Proteostasis
MH  - Protein Kinases/genetics/metabolism
MH  - Plant Proteins/metabolism
MH  - Receptors, Pattern Recognition/metabolism
MH  - Homeostasis
MH  - Ubiquitin-Protein Ligases/genetics/metabolism
MH  - Plant Immunity/physiology
PMC - PMC10397244
COIS- The authors declare no competing interests.
EDAT- 2023/08/03 01:06
MHDA- 2023/08/04 06:43
PMCR- 2023/08/02
CRDT- 2023/08/02 23:16
PHST- 2022/10/20 00:00 [received]
PHST- 2023/07/25 00:00 [accepted]
PHST- 2023/08/04 06:43 [medline]
PHST- 2023/08/03 01:06 [pubmed]
PHST- 2023/08/02 23:16 [entrez]
PHST- 2023/08/02 00:00 [pmc-release]
AID - 10.1038/s41467-023-40364-0 [pii]
AID - 40364 [pii]
AID - 10.1038/s41467-023-40364-0 [doi]
PST - epublish
SO  - Nat Commun. 2023 Aug 2;14(1):4624. doi: 10.1038/s41467-023-40364-0.