PMID- 37534482
OWN - NLM
STAT- Publisher
LR  - 20231024
IS  - 1875-5550 (Electronic)
IS  - 1389-2037 (Linking)
VI  - 24
IP  - 9
DP  - 2023
TI  - REST-restrained lncRNA EPB41L4A-AS2 Modulates Laryngeal Squamous Cell Carcinoma 
      Development via Regulating miR-1254/HIPK2 Pathway.
PG  - 737-757
LID - 10.2174/1389203724666230803094028 [doi]
AB  - BACKGROUND: LncRNAs have been corroborated to exert crucial effects in 
      malignancies, including laryngeal squamous cell carcinoma (LSCC). Nevertheless, 
      the role and mechanism of EPB41L4A- AS2 in LSCC are inadequately investigated and 
      warrant further exploration. METHODS: Relevant database was adopted to analyze 
      the relationship between EPB41L4A-AS2 expression level and tumors. The 
      expressions and relationships of EPB41L4A-AS2, RE-1 silencing transcription 
      factor (REST), miR-1254, and homeodomain interacting protein kinase 2 (HIPK2) in 
      LSCC cells were evaluated by qRT-PCR, Pearson's correlation tests, RNA 
      immunoprecipitation, RNA pull-down assay, chromatin immunoprecipitation, 
      database, and dual-luciferase reporter assay. Following the required 
      transfection, the biological behaviors of LSCC cells were examined using cell 
      function experiments. Meanwhile, the levels of Ki-67 and apoptosis-, and 
      epithelial-mesenchymal transition (EMT) pathway-related proteins were quantified 
      with Western blot. Moreover, xenografts in nude mice were constructed, and the 
      tumor volume and weight were measured. Ki-67 positivity was determined by 
      immunohistochemical staining. RESULTS: EPB41L4A-AS2 and HIPK2 were 
      lower-expressed, yet miR-1254 and REST were higher- expressed in LSCC cells. 
      Pearson's correlation assay results exhibited a positive correlation between 
      HIPK2 and EPB41L4A-AS2 and a negative correlation between HIPK2 and miR-1254. 
      Overexpressed EPB41L4A-AS2 diminished the biological behavior, and repressed the 
      levels of Ki-67 and EMT-related markers in LSCC cells whilst enhancing those of 
      apoptosis-related markers. These aforementioned effects were counteracted by 
      miR-1254 mimic. Moreover, EPB41L4A- AS2 overexpression suppressed the growth of 
      tumors and reduced the positive expression of Ki-67 in nude mice. Besides, 
      miR-1254 aggravated the biological behaviors and elevated the levels of Ki-67 and 
      EMT-related proteins in LSCC cells while reducing the levels of apoptosis-related 
      markers via targeting HIPK2. CONCLUSION: REST-restrained EPB41L4A-AS2 modulates 
      LSCC development via regulating miR-1254/HIPK2 pathway.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Yang, Huijun
AU  - Yang H
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, No.155, Nanjing North Street, Heping District, Shenyang City, 
      Liaoning Province, 110001, China.
FAU - Yu, Gang
AU  - Yu G
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, No.155, Nanjing North Street, Heping District, Shenyang City, 
      Liaoning Province, 110001, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, No.155, Nanjing North Street, Heping District, Shenyang City, 
      Liaoning Province, 110001, China.
FAU - Guo, Xing
AU  - Guo X
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, No.155, Nanjing North Street, Heping District, Shenyang City, 
      Liaoning Province, 110001, China.
LA  - eng
PT  - Journal Article
PL  - United Arab Emirates
TA  - Curr Protein Pept Sci
JT  - Current protein & peptide science
JID - 100960529
SB  - IM
OTO - NOTNLM
OT  - EPB41L4A-AS2
OT  - Ki-67
OT  - RE-1 silencing transcription factor
OT  - homeodomain-interacting protein kinase 2
OT  - laryngeal squamous cell carcinoma
OT  - miR-1254
EDAT- 2023/08/03 06:43
MHDA- 2023/08/03 06:43
CRDT- 2023/08/03 05:35
PHST- 2023/02/07 00:00 [received]
PHST- 2023/05/30 00:00 [revised]
PHST- 2023/07/03 00:00 [accepted]
PHST- 2023/08/03 06:43 [pubmed]
PHST- 2023/08/03 06:43 [medline]
PHST- 2023/08/03 05:35 [entrez]
AID - CPPS-EPUB-133339 [pii]
AID - 10.2174/1389203724666230803094028 [doi]
PST - ppublish
SO  - Curr Protein Pept Sci. 2023;24(9):737-757. doi: 
      10.2174/1389203724666230803094028.