PMID- 37534704
OWN - NLM
STAT- MEDLINE
DCOM- 20230922
LR  - 20230926
IS  - 1097-4598 (Electronic)
IS  - 0148-639X (Linking)
VI  - 68
IP  - 4
DP  - 2023 Oct
TI  - Reliability of confocal corneal microscopy for measurement of dendritic cell 
      density in suspected small fiber neuropathy.
PG  - 460-463
LID - 10.1002/mus.27948 [doi]
AB  - INTRODUCTION/AIMS: Dendritic cells (DCs) and their contacts with corneal nerves 
      are described in animal models of nerve damage. Dendritic cell density (DCD) is a 
      potential marker of immune activity in suspected small-fiber neuropathy (SFN). 
      Here, we aim to evaluate the intra- and inter-rater reliability of DCD 
      measurements in suspected SFN. METHODS: This retrospective study collected DCD 
      from confocal microscopy images from the corneal sub-basal epithelium of the eye 
      from 48 patients (mean age 49.6 +/- 12.1 y, 61% female). Two examiners, each 
      blinded to the other's examinations and measurements, assessed DCD to evaluate 
      inter-rater reliability. For intra-rater reliability, the first examiner 
      performed a second measurement after 14 days. DCs were classified into two cell 
      morphological subtypes: mature and immature. RESULTS: Test-retest reliability for 
      total DCD showed excellent agreement, with an intraclass correlation coefficient 
      of 0.96 and inter-rater reliability intraclass correlation coefficient of 0.77. 
      The immature cell subtype showed excellent intra-rater reliability but lower 
      inter-rater reliability. DISCUSSION: We found that DCD measurements in the 
      corneal sub-basal epithelium are sufficiently reliable for consideration in 
      clinical studies of patients with suspected SFN.
CI  - (c) 2023 The Authors. Muscle & Nerve published by Wiley Periodicals LLC.
FAU - Idiaquez, Juan Francisco
AU  - Idiaquez JF
AUID- ORCID: 0000-0001-9943-8835
AD  - Ellen and Martin Prosserman Centre for Neuromuscular Diseases, Division of 
      Neurology, Department of Medicine, University Health Network, University of 
      Toronto, Toronto, Ontario, Canada.
FAU - Martinez, Rodrigo
AU  - Martinez R
AUID- ORCID: 0000-0003-1679-395X
AD  - Ellen and Martin Prosserman Centre for Neuromuscular Diseases, Division of 
      Neurology, Department of Medicine, University Health Network, University of 
      Toronto, Toronto, Ontario, Canada.
FAU - Barnett-Tapia, Carolina
AU  - Barnett-Tapia C
AUID- ORCID: 0000-0001-5546-0221
AD  - Ellen and Martin Prosserman Centre for Neuromuscular Diseases, Division of 
      Neurology, Department of Medicine, University Health Network, University of 
      Toronto, Toronto, Ontario, Canada.
FAU - Perkins, Bruce A
AU  - Perkins BA
AD  - Division of Endocrinology and Metabolism, University of Toronto, and the 
      Leadership Sinai Centre for Diabetes, Toronto, Ontario, Canada.
FAU - Bril, Vera
AU  - Bril V
AUID- ORCID: 0000-0002-5805-4883
AD  - Ellen and Martin Prosserman Centre for Neuromuscular Diseases, Division of 
      Neurology, Department of Medicine, University Health Network, University of 
      Toronto, Toronto, Ontario, Canada.
LA  - eng
PT  - Journal Article
DEP - 20230803
PL  - United States
TA  - Muscle Nerve
JT  - Muscle & nerve
JID - 7803146
SB  - IM
MH  - Humans
MH  - Female
MH  - Adult
MH  - Middle Aged
MH  - Male
MH  - Reproducibility of Results
MH  - *Small Fiber Neuropathy
MH  - Retrospective Studies
MH  - Microscopy, Confocal/methods
MH  - Dendritic Cells
OTO - NOTNLM
OT  - corneal confocal microscopy
OT  - dendritic cell
OT  - polyneuropathy
OT  - reliability
OT  - small fiber neuropathy
EDAT- 2023/08/03 13:09
MHDA- 2023/09/22 06:42
CRDT- 2023/08/03 07:13
PHST- 2023/07/06 00:00 [revised]
PHST- 2023/01/23 00:00 [received]
PHST- 2023/07/08 00:00 [accepted]
PHST- 2023/09/22 06:42 [medline]
PHST- 2023/08/03 13:09 [pubmed]
PHST- 2023/08/03 07:13 [entrez]
AID - 10.1002/mus.27948 [doi]
PST - ppublish
SO  - Muscle Nerve. 2023 Oct;68(4):460-463. doi: 10.1002/mus.27948. Epub 2023 Aug 3.